Notebook : July 10

Summary:

For regulation system:

• prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing.
• the terminator transformation of yesterday did not work, a second transformation had been done for it.
• The transformation for RBS+LacZ+terminator plasmid into competent cells was performed.
• after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a liquid nutritive medium.

For PSBs sensor system:

• the ligation products were transformed into competent cells and were cultured on solid medium with their specific antibiotics.

Lab work

A.aero/anaerobic regulation system

BioBrick RBS+LacZ+terminator in plasmid PSB1C3

BioBrick RBS+amilCP+terminator in plasmid PSB1C3