- 1 µl Plasmid (pSB1C3-iBB39,pSB1A3-iBB49 or pSB1C3-iBB6315)
- 0.3 µl EcoRI
- 0.3 µl PstI
- 1 µl CutSmart
- 7.4 µl ddH2O
The samples were incubated for 1 h at 37° C.
| Gel electrophoresis |
|
Gel substances
- 1% Agarose gel
- 10 µl RedSafe in 50 ml gel
- x µl Hyper Ladder
Expactations
- Lane 1: 5300 kbp
- Lane 2: 3300 kbp
- Lane 3: 1300 kbp
Worked as expected.
|
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