Team:Paris Saclay/Notebook/July/10

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Contents

1 Notebook : July 10
- 1.1 Lab work
  - 1.1.1 A - Aerobic/Anaerobic regulation system
  - 1.1.2 B - PCB sensor system
    - 1.1.2.1 Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2 protein
    - 1.1.2.2 1 - Transformation of ligation of PSB1C3 and BphA1 or BphR1 or BphR2

Notebook : July 10

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α

Sheng

Transformation of Bba_B0010 of 07/09/13 didn't work. We will do it again.

Protocol : Bacterial transformation

Objective : obtaining biobricks in PSB3K3

1 - Mini and maxi preparation of the culture of Bba_J04450

Abdou

Transformation of Bba_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.

Protocol : Mini and maxi preparation

We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.

B - PCB sensor system

Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2 protein

1 - Transformation of ligation of PSB1C3 and BphA1 or BphR1 or BphR2

Abdou

Protocol : Bacterial transformation

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