• 4 ml cell culture was harvested.
• Pellet was dissolved in 1 mL PBS.
• 0.5 ml glass beads were added.
• Tubes were shaken for 3 min → color shift from brown to green.
• TCA precipitation was performed.

• 4 ml cell culture was harvested.
• Dilution in 300 µL 8M urea buffer.
• Shock freezing of suspension by means of liquid nitrogen.
• Two steel balls were added → Tube was shock freezed again.
• Cell disruption was carried out by 3 times shaking for 30 sec and another shock freezing.

10 ml of cultures grown under different light conditions (green, blue, red, full light spectrum and darkness) were harvested in 15 ml Falcon-tubes (5 min centrifugation at full speed). Supernatants were discarded and pellets frozen in liquid nitrogen.

Frozen cell-pellets were resuspended in 1,7 ml IP-buffer with 8,5 µl PIC and sonicated (48 cycles, 30 sec on, 30 sec off, 4°C, high). Extract was centrifuged at 7000 rcf for 5 min, the supernatant was transferred in a new reaction tube.

1,7 µl supernatant of cell lysis were mixed with 350 µl 70 % TCA and incubated for 20 min on ice. The mixture was centrifuged for 15 min at 20.000 rcf and the pellet washed twice with 1 ml acetone. The pellet was dried at RT and resuspended in 60 µl 8M ureabuffer (15 min 60 °C).

10 µg of each sample were used for SDS-PAGE (Westernblot).