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In the case of normalizing against whole cell protein, it can be observed that the highest amount of eGFP occurred in the cells exposed to darkness followed by the cells grown under green and red light conditions. This result does not agree with the one obtained by normalizing against the OD. Normalization against total cell’s protein amount might be hampered by the fact the amount of protein in the cell is not equal for the different light conditions tested. We therefore conclude that normalization against whole protein amount might not be appropriate. It could well be that in darkness the cells produce proteins, which are absent when grown under light or vice versa. However, further experiments have to be performed to understand these data. Taken together, we found that our promoter is light-inducible and can be activated by blue, green and red light whereof red light yields the best results.