Notebook : July 10

Summary:

For regulation system:

• prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing
• the terminator transformation of yesterday did not work, a second transformation had

been done for it

• The transformation for RBS+LacZ+terminator plasmid into competent cells was

performed.

• after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a

liquid nutritive medium
For PSBs sensor system:

• the ligation products were transformed into competent cells and were cultured on solid

medium with their specific antibiotics

Lab work

constructing