Team:Macquarie Australia/Protocols/SDS Page

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SDS Page

Pelleted cells were resuspended in 200 uL Milli-Q H2O.


Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.


Using a Hamilton syringe, the cells were sheared.


Centrifuged the preparations for 3 mins @ 13,000 rpm.


Loaded 20 uL of the supernatant in to the gel.


Electrophoresis was conducted at constant voltage (200 V) for one hour.

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