Team:Bielefeld-Germany

From 2013.igem.org

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<h2>Introduction </h2>
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Over 100 years ago the British botanist M. C. Potter discovered electro-chemical reactions by anaerobe microbial degradation processes. This was the milestone for the first thoughts of developing a system for degradation of substances with additional generating of electricity.
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There is a growing interest in the use of alternative energy sources. Decreasing use of fossil fuels in the context of atmospheric greenhouse gas reduction, coupled with the recent reduction of nuclear power production in Germany have encouraged the search for alternative energy production processes. Generation of electricity at large scales can have negative impacts on local environments and the use of energy storage devices on a small scale holds the risk of release of toxic pollutants from batteries.
 In consideration of these issues, the iGEM team of Bielefeld University is undertaking a project called "Microbial fuel cell" (MFC) this year. The goal of this project is to use bacteria for direct energy production from organic substrates.
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Unlike conventional batteries, a Biobattery is environmentally friendly and easy to “charge developing countries. In contrast to wind and solar energy, electricity production is controlled in the MFC by a targeted substrate supply providing direct energy when it is required and avoiding the need for complex storage systems. Exhibiting rapid growth with the addition of simple substrates, bacteria are available within minimal time and in a large” through substrate refilling. Due to its simple design, the MFC could be used in areas with occasional power shortages as a back up energy generation system, for example in quantity. The iGEM team at Bielefeld wants to test different bacteria strains as electron donors, as well as design our own MFC to improve electron transfer and electricity production. Using BioBrick concept and ''Escherichia coli'' as a model organism, we investigate different genetic approaches in order to ensure efficient power generation from the microbial system.
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In addition to the electrical power production on the anode, we will attempt to use the reduction potential of the MFC-cathode for a bioremediation strategy.
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There are different fields of application to use a MFC.
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For example the usage of the MFC in a wastewater treatment plant to produce electricity by degradation substances which are in the water. This could be an important move to switch the wastewater from an uninteresting, stinky broth to an interesting energy-gaining and so more effective degradation process. Thereby it would be possible to remove recesses of medicals like ethylestradiol or toxic heavy metals like even uran.
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Another application of an MFC is the demineralization of seawater. This can be done without or with very low energy input to assemble drinking water out of seawater.
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<h1><i>E</i>col<i>ectricity</i> -  <i>current</i>ly available</h1>
<br><br>
<br><br>
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<h1>Electric energy by genetically modified E. coli in a self-designed and constructed microbial fuell cell</h1>
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<h3>Project</h3>
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<h2>Project</h2>
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<p>This year the iGEM team 2013 decided to do research on a microbial fuel cell (MFC). This project includes genetically modification of Escherichia coli and engineering of a suitable fuel cell to gain electricity. </p>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Abstract" class="info">Read more</a>
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<h3>Team</h3>
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<h2>Team</h2>
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<p>Our team exists of ten bachelor and master students which study molecular biotechnology and genome based system biology. Get to know us better.</p>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Abstract" class="info">Read more</a>
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document.write(unescape("%3Cscript src='http://twitterforweb.com/twitterbox.js?username=@iGEM_Bielefeld&settings=1,1,3,236,650,ffffff,1,ffffff,101010,1,1,336699' type='text/javascript'%3E%3C/script%3E"));</script>Created by: <a href="http://twitterforweb.com" target="_blank">twitter website widget</a></div>
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<h3>Biosafety</h3>
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<img src="https://static.igem.org/mediawiki/2013/c/c6/IGEM_Bielefeld_2013_Biosafety_E.coli_bewaffnet_safe_2..png" height="200px" style="display: block; margin: 0 auto;">
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<h2>Biosafety</h2>
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<p>Biosafety is an essential aspect when you work with living organisms which could possibly get out of your application by damage or incorrect handling. We designed biosafety systems which counter this risk.</p>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Abstract" class="info">Read more</a>
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<h3>Results</h3>
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<h2>Results</h2>
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<p>Our aim was to produce electricity with our application which we finally achieved successfully. On that long way we got many data which we analyzed and formatted into graphics. Take a look.</p>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Abstract" class="info">Read more</a>
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<h2>Human Practice</h2>
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<p>It is important to get out of the lab and tell the public about your project and synthetic biology. Therefore we arranged events and visited conferences to get in touch with the public.</p>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Abstract" class="info">Read more</a>
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<h2>Labjournal</h2>
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<p>We wrote all the experiments we did into a labjournal. We digitalized the labjournal as everyone can comprehend and reproduce our experiments. </p>
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<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Abstract" class="info">Read more</a>
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<h1>Achievements</h1>
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Design and construction of a [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC MFC]
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Design and construction of a biodegradable [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC#Results 3D-printed MFC]
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Optimization of the [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC MFC]
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Construction of [https://2013.igem.org/Team:Bielefeld-Germany/Project/Porins OprF] expressing Biobricks
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Construction of [https://2013.igem.org/Team:Bielefeld-Germany/Project/GldA glyceroldehydrogenase] expressing Biobricks
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Construction of [https://2013.igem.org/Team:Bielefeld-Germany/Project/Riboflavine riboflavin] expressing Biobricks
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Construction of [https://2013.igem.org/Team:Bielefeld-Germany/Project/Cytochromes cytochrome] expressing Biobricks
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Improve of the Biobrick [http://parts.igem.org/Part:BBa_K914014?title=Part:BBa_K914014 BBa_K914014]
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Development of three auxotrophic degradation [https://2013.igem.org/Team:Bielefeld-Germany/Biosafety/Biosafety_System Biosafety systems]
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Help another [https://2013.igem.org/Team:Bielefeld-Germany/Collaborations team]
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[[Image:Bielefeld-Germany2013_Box_checked.png|60px]] Production of electricity via the [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC#Results MFC]
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Revision as of 22:26, 4 October 2013





Ecolectricity - currently available



Electric energy by genetically modified E. coli in a self-designed and constructed microbial fuell cell




Project

Project

This year the iGEM team 2013 decided to do research on a microbial fuel cell (MFC). This project includes genetically modification of Escherichia coli and engineering of a suitable fuel cell to gain electricity.

Read more

Team

Team

Our team exists of ten bachelor and master students which study molecular biotechnology and genome based system biology. Get to know us better.

Read more

Biosafety

Biosafety

Biosafety is an essential aspect when you work with living organisms which could possibly get out of your application by damage or incorrect handling. We designed biosafety systems which counter this risk.

Read more

Results

Results

Our aim was to produce electricity with our application which we finally achieved successfully. On that long way we got many data which we analyzed and formatted into graphics. Take a look.

Read more

Human Practice

Human Practice

It is important to get out of the lab and tell the public about your project and synthetic biology. Therefore we arranged events and visited conferences to get in touch with the public.

Read more

Labjournal

Labjournal

We wrote all the experiments we did into a labjournal. We digitalized the labjournal as everyone can comprehend and reproduce our experiments.

Read more

Achievements

Bielefeld-Germany2013 Box checked.png Design and construction of a MFC
Bielefeld-Germany2013 Box checked.png Design and construction of a biodegradable 3D-printed MFC
Bielefeld-Germany2013 Box checked.png Optimization of the MFC
Bielefeld-Germany2013 Box checked.png Construction of OprF expressing Biobricks
Bielefeld-Germany2013 Box checked.png Construction of glyceroldehydrogenase expressing Biobricks
Bielefeld-Germany2013 Box checked.png Construction of riboflavin expressing Biobricks
Bielefeld-Germany2013 Box checked.png Construction of cytochrome expressing Biobricks
Bielefeld-Germany2013 Box checked.png Improve of the Biobrick [http://parts.igem.org/Part:BBa_K914014?title=Part:BBa_K914014 BBa_K914014]
Bielefeld-Germany2013 Box checked.png Development of three auxotrophic degradation Biosafety systems
Bielefeld-Germany2013 Box checked.png Help another team
Bielefeld-Germany2013 Box checked.png Production of electricity via the MFC


Retrieved from "http://2013.igem.org/Team:Bielefeld-Germany"