Team:Colombia Uniandes/How to parts

From 2013.igem.org

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Revision as of 19:22, 25 September 2013

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**Primers used to construct Nicko & Chimi**
Name	Sequence	Identification Number
FW XbaI-BAP2	CACCTCTAGAGAGCATTAATGCAAGTCGAGAAAGG	19
FW XbaI-GAL1	CACCTCTAGAGGCTATATTGAAGTACGGATTAG	15
FW Fusion GAL1-KOZAC-mCherry	GGAAACTAGACCCGCCGCCACCATGGAGATGGTGAGCAAGGGCGAGG	17
RV Fusion GAL1-KOZAC-mCherry	CCTCGCCCTTGCTCACCATCTCCATGGTGGCGGCGGGTCTAGTTTCC	13
FW Fusion BAP2-KOZAC-GAL4	CTCAAGTGATTTTAGAACCCGCCGCCACCATGGAGATGAAGCTACTGTCTTC	11
RV Fusion BAP2-KOZAC-GAL4	GAAGACAGTAGCTTCATCTCCATGGTGGCGGCGGGTTCTAAAATCACTTGAG	3
FW FUSION GAL4-VP16-1	CAGTTGACTGTATCTTCGAGGTCGACCATTCAGATCTCACGAGCCGCCCCCCCGACCG	1
RV FUSION GAL4-VP16-1	CGGTCGGGGGGGCGGCTCGTGAGATCTGAATGGTCGACCTCGAAGATACAGTCAACTG	5
FW FUSION VP6-GCR	CCTACGGCGCTCTGGATATGATCTCTCGAGCCATTCAGCAAGCCACTGCAGG	9
RV FUSION VP6-GCR	CCTGCAGTGGCTTGCTGAATGGCTCGAGAGATCATATCCAGAGCGCCGTAGG	6
FW Fusion GCR-yeastTerminador	GCTTCTGTTTCATCAAAAATGAAAAGTGAATATCCAAACGAGAATGTCAATGG	35
RV Fusion GCR-yeastTerminador	CCATTGACATTCTCGTTTGGATATTCACTTTTCATTTTTGATGAAACAGAAGC	34
FW Fusion mCherry-yeastTerminador	GGACGAGCTGTACAAGTAATAAAAAGTGAATATCCAAACGAGAATGTCAATGG	33
RV Fusion mCherry-yeastTerminador	CCATTGACATTCTCGTTTGGATATTCACTTTTTATTACTTGTACAGCTCGTCC	32
FW HoxN	ATGGAAGCACTGCCGAACGATT	42
RV HoxNconColamRfP	GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG	44
FW mRFPconColaHoxN	GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA	41
RV mRFPconXba	ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC	38
FW Prcna	GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT	43
RV Prcna	TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT	39
J23117+B0034+RcnR FW	GACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCAAAGAGGAGAAAATGTCTCATACAATC	23
J23117+B0034+RcnR RV	GATTGTATGAGACATTTTCTCCTCTTTGCTAGCACAATCCCTAGGACTGAGCTAGCTGTC	21

Retrieved from "http://2013.igem.org/Team:Colombia_Uniandes/How_to_parts"

@@ Line 7: / Line 7: @@
 |-
-| style="text-align: center;" |FW XbaI-BAP2
+| style="text-align: left;" |FW XbaI-BAP2
 | style="text-align: left;" |CACCTCTAGAGAGCATTAATGCAAGTCGAGAAAGG
 | style="text-align: center;" |19
 |-
-| style="text-align: center;" |FW XbaI-GAL1
+| style="text-align: left;" |FW XbaI-GAL1
 | style="text-align: left;" |CACCTCTAGAGGCTATATTGAAGTACGGATTAG
 | style="text-align: center;" |15
 |-
-| style="text-align: center;" |FW Fusion GAL1-KOZAC-mCherry
+| style="text-align: left;" |FW Fusion GAL1-KOZAC-mCherry
 | style="text-align: left;" |GGAAACTAGACCCGCCGCCACCATGGAGATGGTGAGCAAGGGCGAGG
 | style="text-align: center;" |17
 |-
-| style="text-align: center;" |RV Fusion GAL1-KOZAC-mCherry
+| style="text-align: left;" |RV Fusion GAL1-KOZAC-mCherry
 | style="text-align: left;" |CCTCGCCCTTGCTCACCATCTCCATGGTGGCGGCGGGTCTAGTTTCC
 | style="text-align: center;" |13
 |-
-| style="text-align: center;" |FW Fusion BAP2-KOZAC-GAL4
+| style="text-align: left;" |FW Fusion BAP2-KOZAC-GAL4
 | style="text-align: left;" |CTCAAGTGATTTTAGAACCCGCCGCCACCATGGAGATGAAGCTACTGTCTTC
 | style="text-align: center;" |11
 |-
-| style="text-align: center;" |RV Fusion BAP2-KOZAC-GAL4
+| style="text-align: left;" |RV Fusion BAP2-KOZAC-GAL4
 | style="text-align: left;" |GAAGACAGTAGCTTCATCTCCATGGTGGCGGCGGGTTCTAAAATCACTTGAG
 | style="text-align: center;" |3
 |-
-| style="text-align: center;" |FW FUSION GAL4-VP16-1
+| style="text-align: left;" |FW FUSION GAL4-VP16-1
 | style="text-align: left;" |CAGTTGACTGTATCTTCGAGGTCGACCATTCAGATCTCACGAGCCGCCCCCCCGACCG
 | style="text-align: center;" |1
 |-
-| style="text-align: center;" |RV FUSION GAL4-VP16-1
+| style="text-align: left;" |RV FUSION GAL4-VP16-1
 | style="text-align: left;" |CGGTCGGGGGGGCGGCTCGTGAGATCTGAATGGTCGACCTCGAAGATACAGTCAACTG
 | style="text-align: center;" |5
 |-
-| style="text-align: center;" |FW FUSION VP6-GCR
+| style="text-align: left;" |FW FUSION VP6-GCR
 | style="text-align: left;" |CCTACGGCGCTCTGGATATGATCTCTCGAGCCATTCAGCAAGCCACTGCAGG
 | style="text-align: center;" |9
 |-
-| style="text-align: center;" |RV FUSION VP6-GCR
+| style="text-align: left;" |RV FUSION VP6-GCR
 | style="text-align: left;" |CCTGCAGTGGCTTGCTGAATGGCTCGAGAGATCATATCCAGAGCGCCGTAGG
 | style="text-align: center;" |6
 |-
-| style="text-align: center;" |FW Fusion GCR-yeastTerminador
+| style="text-align: left;" |FW Fusion GCR-yeastTerminador
 | style="text-align: left;" |GCTTCTGTTTCATCAAAAATGAAAAGTGAATATCCAAACGAGAATGTCAATGG
 | style="text-align: center;" |35
 |-
-| style="text-align: center;" |RV Fusion GCR-yeastTerminador
+| style="text-align: left;" |RV Fusion GCR-yeastTerminador
 | style="text-align: left;" |CCATTGACATTCTCGTTTGGATATTCACTTTTCATTTTTGATGAAACAGAAGC
 | style="text-align: center;" |34
 |-
-| style="text-align: center;" |FW Fusion mCherry-yeastTerminador
+| style="text-align: left;" |FW Fusion mCherry-yeastTerminador
 | style="text-align: left;" |GGACGAGCTGTACAAGTAATAAAAAGTGAATATCCAAACGAGAATGTCAATGG
 | style="text-align: center;" |33
 |-
-| style="text-align: center;" |RV Fusion mCherry-yeastTerminador
+| style="text-align: left;" |RV Fusion mCherry-yeastTerminador
 | style="text-align: left;" |CCATTGACATTCTCGTTTGGATATTCACTTTTTATTACTTGTACAGCTCGTCC
 | style="text-align: center;" |32
 |-
-| style="text-align: center;" |FW HoxN
+| style="text-align: left;" |FW HoxN
 | style="text-align: left;" |ATGGAAGCACTGCCGAACGATT
 | style="text-align: center;" |42
 |-
-| style="text-align: center;" |RV HoxNconColamRfP
+| style="text-align: left;" |RV HoxNconColamRfP
 | style="text-align: left;" |GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG
 | style="text-align: center;" |44
 |-
-| style="text-align: center;" |FW mRFPconColaHoxN
+| style="text-align: left;" |FW mRFPconColaHoxN
 | style="text-align: left;" |GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA
 | style="text-align: center;" |41
 |-
-| style="text-align: center;" |RV mRFPconXba
+| style="text-align: left;" |RV mRFPconXba
 | style="text-align: left;" |ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC
 | style="text-align: center;" |38
 |-
-| style="text-align: center;" |FW Prcna
+| style="text-align: left;" |FW Prcna
 | style="text-align: left;" |GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT
 | style="text-align: center;" |43
 |-
-| style="text-align: center;" |RV Prcna
+| style="text-align: left;" |RV Prcna
 | style="text-align: left;" |TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT
 | style="text-align: center;" |39
 |-
-| style="text-align: center;" |J23117+B0034+RcnR FW
+| style="text-align: left;" |J23117+B0034+RcnR FW
 | style="text-align: left;" |GACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCAAAGAGGAGAAAATGTCTCATACAATC
 | style="text-align: center;" |23
 |-
-| style="text-align: center;" |J23117+B0034+RcnR RV
+| style="text-align: left;" |J23117+B0034+RcnR RV
 | style="text-align: left;" |GATTGTATGAGACATTTTCTCCTCTTTGCTAGCACAATCCCTAGGACTGAGCTAGCTGTC
 | style="text-align: center;" |21
 |-
 |}