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Team:ETH Zurich/Data Page
From 2013.igem.org
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<h1>Our Favorite New Parts</h1> | <h1>Our Favorite New Parts</h1> | ||
<p align= "justify"> | <p align= "justify"> | ||
| - | [http://parts.igem.org/Part:BBa_K1216002 Main Page] - Acetyl esterase (AES), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate <br> | + | 1. [http://parts.igem.org/Part:BBa_K1216002 Main Page] - Acetyl esterase (AES), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate <br> |
| - | [http://parts.igem.org/Part:BBa_K1216005 MainPage] - Alkaline phosphatase with His tag and TEV cleavage site (phoA), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate <br> | + | 2. [http://parts.igem.org/Part:BBa_K1216005 MainPage] - Alkaline phosphatase with His tag and TEV cleavage site (phoA), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate <br> |
| - | [http://parts.igem.org/Part:BBa_K1216007 MainPage] - pLuxR1 mutated promoter, BBa_K1216007 : the promoter has an EC<sub>50</sub> of 6.462 nM in liquid culture and 17'814nM on agar plates. This sensitivity is shifted 300'000 fold compared to the original BBa_R0062 pLuxR</p> | + | 3. [http://parts.igem.org/Part:BBa_K1216007 MainPage] - pLuxR1 mutated promoter, BBa_K1216007 : the promoter has an EC<sub>50</sub> of 6.462 nM in liquid culture and 17'814nM on agar plates. This sensitivity is shifted 300'000 fold compared to the original BBa_R0062 pLuxR</p> |
<h1>Characterized Pre-existing Parts :</h1> | <h1>Characterized Pre-existing Parts :</h1> | ||
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1. [http://parts.igem.org/Part:BBa_R0062:Experience Experience] - pLuxR wild type, BBa_R0062 : phoA gene from <i>Citrobacter</i>. We did Michealis-Menten kinetics of the phoA enzyme and also showed the conversion of PNP (para-nitrophenol phosphate) in a yellow analog precipitate.]<br> | 1. [http://parts.igem.org/Part:BBa_R0062:Experience Experience] - pLuxR wild type, BBa_R0062 : phoA gene from <i>Citrobacter</i>. We did Michealis-Menten kinetics of the phoA enzyme and also showed the conversion of PNP (para-nitrophenol phosphate) in a yellow analog precipitate.]<br> | ||
| - | 2.[http://parts.igem.org/Part:BBa_J61032:Experience Experience] - Alkaline phosphatase, BBa_J61032 : phoA gene originated from <i>Citrobacter</i>. We did Michealis-Menten kinetics of the phoA enzyme and also showed the conversion of PNP (para-nitrophenol phosphate) in a yellow analog precipitate</p> | + | 2. [http://parts.igem.org/Part:BBa_J61032:Experience Experience] - Alkaline phosphatase, BBa_J61032 : phoA gene originated from <i>Citrobacter</i>. We did Michealis-Menten kinetics of the phoA enzyme and also showed the conversion of PNP (para-nitrophenol phosphate) in a yellow analog precipitate</p> |
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<p align= "justify"> | <p align= "justify"> | ||
| - | [http://parts.igem.org/Part:BBa_K1216000 MainPage] - β-Glucuronidase (gusA), BBa_K1216000 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.<br> | + | 1. [http://parts.igem.org/Part:BBa_K1216000 MainPage] - β-Glucuronidase (gusA), BBa_K1216000 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.<br> |
| - | [http://parts.igem.org/Part:BBa_K1216003 MainPage] - β-N-Acetylglucosaminidase (nagZ), BBa_K1216003 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.<br> | + | 2. [http://parts.igem.org/Part:BBa_K1216003 MainPage] - β-N-Acetylglucosaminidase (nagZ), BBa_K1216003 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.<br> |
| - | [http://parts.igem.org/Part:BBa_K1216006 Main Page] - Acetyl esterase (AES)with His-Tag and TEV cleavage site, BBa_K1216006 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate <br> </p> | + | 3. [http://parts.igem.org/Part:BBa_K1216006 Main Page] - Acetyl esterase (AES)with His-Tag and TEV cleavage site, BBa_K1216006 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate <br> </p> |
| - | [http://parts.igem.org/Part:BBa_K1216004 MainPage] - β-Glucuronidase (gusA)with HIS-Tag and TEV cleavage site, BBa_K1216004 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.<br> | + | 4. [http://parts.igem.org/Part:BBa_K1216004 MainPage] - β-Glucuronidase (gusA)with HIS-Tag and TEV cleavage site, BBa_K1216004 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.<br> |
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{{:Team:ETH_Zurich/templates/footer}} | {{:Team:ETH_Zurich/templates/footer}} | ||
Revision as of 18:44, 2 October 2013
Contents |
Gene circuit
Our Favorite New Parts
1. [http://parts.igem.org/Part:BBa_K1216002 Main Page] - Acetyl esterase (AES), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate
2. [http://parts.igem.org/Part:BBa_K1216005 MainPage] - Alkaline phosphatase with His tag and TEV cleavage site (phoA), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate
3. [http://parts.igem.org/Part:BBa_K1216007 MainPage] - pLuxR1 mutated promoter, BBa_K1216007 : the promoter has an EC50 of 6.462 nM in liquid culture and 17'814nM on agar plates. This sensitivity is shifted 300'000 fold compared to the original BBa_R0062 pLuxR
Characterized Pre-existing Parts :
1. [http://parts.igem.org/Part:BBa_R0062:Experience Experience] - pLuxR wild type, BBa_R0062 : phoA gene from Citrobacter. We did Michealis-Menten kinetics of the phoA enzyme and also showed the conversion of PNP (para-nitrophenol phosphate) in a yellow analog precipitate.]
2. [http://parts.igem.org/Part:BBa_J61032:Experience Experience] - Alkaline phosphatase, BBa_J61032 : phoA gene originated from Citrobacter. We did Michealis-Menten kinetics of the phoA enzyme and also showed the conversion of PNP (para-nitrophenol phosphate) in a yellow analog precipitate
Characterized New Parts
1. [http://parts.igem.org/Part:BBa_K1216000 MainPage] - β-Glucuronidase (gusA), BBa_K1216000 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.
2. [http://parts.igem.org/Part:BBa_K1216003 MainPage] - β-N-Acetylglucosaminidase (nagZ), BBa_K1216003 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.
3. [http://parts.igem.org/Part:BBa_K1216006 Main Page] - Acetyl esterase (AES)with His-Tag and TEV cleavage site, BBa_K1216006 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate
4. [http://parts.igem.org/Part:BBa_K1216004 MainPage] - β-Glucuronidase (gusA)with HIS-Tag and TEV cleavage site, BBa_K1216004 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.
