Team:ETH Zurich/Experiments 7

From 2013.igem.org

(Difference between revisions)
Line 3: Line 3:
<h1>What about those <i>hydrolases</i> ?</h1>
<h1>What about those <i>hydrolases</i> ?</h1>
-
 
-
[[File:Hydrolases.png|900px|left|thumb|<b>Figure.1 Enzyme substrate reaction of the hydrolases and respective substrates</b>]]
 
We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).<br><br> On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.<br>
We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).<br><br> On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.<br>
<br clear="all"/>
<br clear="all"/>

Revision as of 12:59, 13 September 2013

Header2.png
80px-Eth igem logo.png

What about those hydrolases ?

We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).

On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.

Colorimetric response in liquid culture


Colorimetric response on LB-Agar


Beta-galactosidase(LacZ)


Fig.5: X-Gal on LacZ expressing colony
Fig.6: Beta-green-X-gal on LacZ expressing colony



Alkaline phosphatase(PhoA)


Fig.2: NPP on PhoA expressing colony



Acetyl esterase(Aes)


Fig.1: Magenta butyrate on Aes expressing colony



Glycoside hydrolase(NagZ)


Fig.4: X-Glucnac on NagZ expressing colony



Beta-glucuronidase(GusA)


Fig.3 Magenta glucuronide on GusA expressing colony