We achieved

Pre-Processing:

- Characterize the AHL diffusion on agar plates and define, with the predition from the model and the experimental results, the distance between colonies, the strengh of the LuxI promoter and the incubation time for the diffusion.
- To astablish several AHL gradients from different mines on one plate, intersections of gradients result in higher AHL levels for the detection of 1, 2 or 3 mines.
- Make a spatio-temporal model of AHL difussion

Processing:

- To make a promoter libary with 8 different EC50 from 0.02nM to 6482nM in lquid culture and form 4.45nM to 12'555nM on agar plates.
- Find a analytical solution for the EC50 of the promoter we need to find.
- We measured the dose-response of those promoters in liquid culture and on agar plates.
- By the prediction of the model we chooose xy to be the right one for our set-up.
- To implement several different reporters in one construct.

A proof-of-principle using a GFP reporter.

Optimization:

- Identification of the leakyness source.
- Optimize the circuit to reduce the leakiness by introduction of a negative feedback loop.

Output:

- Different colormetric response for all hydrolases.
- Characterize the hydrolases by Michaelis-Menten kinetics.


A preliminary game with the hydrolases.

Establish a spatio-temporal model of the proof-of-principle and the final game.

We thank our sponsors: