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Team:ETH Zurich/pre proc
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<h1>LuxR signaling system </h1> | <h1>LuxR signaling system </h1> | ||
| - | [[File:LuxR system.png| | + | [[File:LuxR system.png|500px|center|Thumb|<b>Figure 1. LuxR signaling system</b>|]] |
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| + | As our system is based on a sender-receiver module, we use the lux signalling system in our bio-game. In <i>Vibriofischeri</i>, the <i>lux</i> gene encodes for the enzyme that catalyses the production of the signalling molecule N-acyl-homoserine lactone, AHL. The AHl molecule is able to diffuse in and out of cells. This molecule then binds to the inactive luxR protein. Binding of AHL to the luxR forms an active AHL-luxR complex. This complex then binds to the P<sub>lux</sub> promoter and drives the gene expression forward. | ||
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| + | In the bio-game, the mines are the sender cells and the non-mines are the receiver cells. The mines constitutively express LuxI and hence AHL. This molecule then diffuses through the agar and can be processed by the non-mines. Depending on the amount of AHL that is processed, different expression of output colors give the player the cue for the next move in the bio-game. | ||
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Revision as of 07:30, 27 October 2013
LuxR signaling system
As our system is based on a sender-receiver module, we use the lux signalling system in our bio-game. In Vibriofischeri, the lux gene encodes for the enzyme that catalyses the production of the signalling molecule N-acyl-homoserine lactone, AHL. The AHl molecule is able to diffuse in and out of cells. This molecule then binds to the inactive luxR protein. Binding of AHL to the luxR forms an active AHL-luxR complex. This complex then binds to the Plux promoter and drives the gene expression forward.
In the bio-game, the mines are the sender cells and the non-mines are the receiver cells. The mines constitutively express LuxI and hence AHL. This molecule then diffuses through the agar and can be processed by the non-mines. Depending on the amount of AHL that is processed, different expression of output colors give the player the cue for the next move in the bio-game.
