Team:Marburg

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The iGEM Marburg proudly presents: PHAECTORY
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{{:Team:Marburg/Template:ContentStart}}The use of proteins in medical treatment and diagnosis is steadily increasing. Many of these proteins (e.g. '''antibodies''') have a complex biological structure, which complicates their production. Also, these proteins need to be highly pure. Therefore, a major challenge is the development of systems that produce complex proteins with high purity - '''best at low costs'''!
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The sunlight-driven microalgae ''[[Team:Marburg/Project:Ptricornutum|Phaeodactylum tricornutum]]'' has been used for the production of complex proteins. A major '''[[Team:Marburg/Project:Challenge|advantage]]''' of the algae is its ability to secrete proteins directly into the medium. This feature would greatly simplify purification of recombinant proteins, and lower production costs! Here we take advantage of the excellent secretion abilities of ''P. tricornutum'', and make this organism accessible to synthetic biology and the iGEM competition ([https://2013.igem.org/Team:Marburg/Project PHAECTORY]).
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<h1 class="content-title">Project Description<span style="float: right;">
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'''Producing high-value proteins in ''Phaeodactylum tricornutum'' '''
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Furthermore, we used a light-inducible promoter which we have characterized quantitatively in order to control protein expression in PHAECTORY ([https://2013.igem.org/Team:Marburg/Project:lightcontrol Light control]). Also, we introduced a transferable element to the registry, which allows autonomous targeting of proteins to the inner surface of cell membranes ([https://2013.igem.org/Team:Marburg/Project:RFP Improve a Brick]). We hope you enjoy reading about our project!
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The diatom ''Phaeodactylum tricornutum'' is a widely spread organism in marine waters. Together with about 6000 other species, diatoms belong to the phylum of heterokonts. As a group of great ecological relevance diatoms are responsible for up to 20% of the global CO<sub>2</sub> fixation and generate about 40 % of the marine biomass of primary producers (Falkowski ''et al.'', 1998, Field ''et al.'', 1998).
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In addition, diatoms represent an important source of lipids and silicate making them interesting for various biotechnological applications e.g. in biofuel industry, food industry and nanofabrication.
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“Normal” biotechnology is applied in bacteria and yeast.  Both need to be fed with cost intensive chemicals and the purification of the products e.g. Proteins is very time consuming and difficult, due to the fact that the cells need to be cracked or chemicals in the medium need to be removed.
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In contrast, ''P. tricornutum'' is a green organism living from CO<sub>2</sub> and light and love. The purification of the desired product is much easier, because the proteins are secreted to the surrounding medium. As a plant, microalgae don’t secret many other substances and therefore our product is already in a high purity available (Hempel ''et al.'', 2011).
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In order to introduce this organism as a chassis to the iGEM community, we build a toolbox of expression vectors, promoters, selection markers, reporter proteins and signal peptides for ''P. tricornutum''. This allows every future iGEM team to produce their own complex proteins, which can easily be purified.
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<center><b><a href="http://www.youtube.com/embed/mN9WnJ14-Yo" target="_blank">Video Introduction</a></b></center>
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<center><b><a href="https://2013.igem.org/Team:Marburg/Human_Practice">Human Practice</a></b></center>
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            <a href="https://2013.igem.org/Team:Marburg/Human_Practice:DayofSynthBiology">
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            <a href="https://2013.igem.org/Team:Marburg/Human_Practice/Conferences:BioTech2020plus">
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            <a href="https://2013.igem.org/Team:Marburg/Human_Practice:Debate">
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<h2>Our Sponsors</h2><br />
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          href="http://www.synmikro.com/" target="_blank" alt="Synmikro" title="Synmikro" />
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          href="http://www.abbvie.com/" target="_blank" alt="Abbvie" title="Abbvie" />
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          href="http://www.healthcare.siemens.com/" target="_blank" alt="Siemens" title="Siemens" />
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          href="http://www.erasynbio.eu/" target="_blank" alt="ERA SynBio" title="ERA SynBio" />
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Latest revision as of 18:42, 28 October 2013

The iGEM Marburg proudly presents: PHAECTORY

The use of proteins in medical treatment and diagnosis is steadily increasing. Many of these proteins (e.g. antibodies) have a complex biological structure, which complicates their production. Also, these proteins need to be highly pure. Therefore, a major challenge is the development of systems that produce complex proteins with high purity - best at low costs!

The sunlight-driven microalgae Phaeodactylum tricornutum has been used for the production of complex proteins. A major advantage of the algae is its ability to secrete proteins directly into the medium. This feature would greatly simplify purification of recombinant proteins, and lower production costs! Here we take advantage of the excellent secretion abilities of P. tricornutum, and make this organism accessible to synthetic biology and the iGEM competition (PHAECTORY).

Furthermore, we used a light-inducible promoter which we have characterized quantitatively in order to control protein expression in PHAECTORY (Light control). Also, we introduced a transferable element to the registry, which allows autonomous targeting of proteins to the inner surface of cell membranes (Improve a Brick). We hope you enjoy reading about our project!

Video Introduction
YouTube Video
Human Practice


Our Sponsors


Our Sponsors Synmikro Hessen Trade & Invest Abbvie Siemens ERA SynBio

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