Team:Marburg

From 2013.igem.org

(Difference between revisions)
m
m
 
(36 intermediate revisions not shown)
Line 2: Line 2:
{{:Team:Marburg/Template:ContentTitle}}
{{:Team:Marburg/Template:ContentTitle}}
The iGEM Marburg proudly presents: PHAECTORY
The iGEM Marburg proudly presents: PHAECTORY
-
{{:Team:Marburg/Template:ContentStart}}
+
{{:Team:Marburg/Template:ContentStart}}The use of proteins in medical treatment and diagnosis is steadily increasing. Many of these proteins (e.g. '''antibodies''') have a complex biological structure, which complicates their production. Also, these proteins need to be highly pure. Therefore, a major challenge is the development of systems that produce complex proteins with high purity - '''best at low costs'''!
-
The use of proteins in medical treatment and diagnosis is steadily increasing. Many pharmaceutically relevant proteins, such as antibodies, have a complex biological structure with specific posttranslational modifications. Furthermore, these proteins need to be highly pure because of their use in diagnostics and treatment. Therefore, a major challenge is the development of systems that produce highly pure, posttranslational modified pharmaceutical proteins - best at low costs.
+
-
Recently, the sunlight-driven microalgae ''Phaeodactylum tricornutum'' has been established as an elegant tool for the production of proteins (PHAECTORY). A major - however yet unchallenged - advantage of this system is the production of advanced proteins with specific posttranslational modifications. A further major advantage is the ability of their direct secretion into the medium, which allows easy purification with low effort and costs. Our mission is to challenge PHAECTORY as a system for the convenient production of antibodies. We want to take advantage of the excellent secretion and ability of posttranslational modification and make the system accessible to following iGEM teams. [[Team:Marburg/Project|Have a look on our project]].
+
The sunlight-driven microalgae ''[[Team:Marburg/Project:Ptricornutum|Phaeodactylum tricornutum]]'' has been used for the production of complex proteins. A major '''[[Team:Marburg/Project:Challenge|advantage]]''' of the algae is its ability to secrete proteins directly into the medium. This feature would greatly simplify purification of recombinant proteins, and lower production costs! Here we take advantage of the excellent secretion abilities of ''P. tricornutum'', and make this organism accessible to synthetic biology and the iGEM competition ([https://2013.igem.org/Team:Marburg/Project PHAECTORY]).
 +
 
 +
Furthermore, we used a light-inducible promoter which we have characterized quantitatively in order to control protein expression in PHAECTORY ([https://2013.igem.org/Team:Marburg/Project:lightcontrol Light control]). Also, we introduced a transferable element to the registry, which allows autonomous targeting of proteins to the inner surface of cell membranes ([https://2013.igem.org/Team:Marburg/Project:RFP Improve a Brick]). We hope you enjoy reading about our project!
<html></p>
<html></p>
Line 20: Line 21:
<div class="home">
<div class="home">
<div class="home_youtube">
<div class="home_youtube">
 +
<center><b><a href="http://www.youtube.com/embed/mN9WnJ14-Yo" target="_blank">Video Introduction</a></b></center>
<a href="http://www.youtube.com/embed/mN9WnJ14-Yo" target="_blank">
<a href="http://www.youtube.com/embed/mN9WnJ14-Yo" target="_blank">
<img src="https://static.igem.org/mediawiki/2013/3/31/Mr-igem-youtube.png" alt="YouTube Video" />
<img src="https://static.igem.org/mediawiki/2013/3/31/Mr-igem-youtube.png" alt="YouTube Video" />
Line 25: Line 27:
</div>
</div>
<div class="home_cycle">
<div class="home_cycle">
 +
<center><b><a href="https://2013.igem.org/Team:Marburg/Human_Practice">Human Practice</a></b></center>
<!--[if !IE]> -->
<!--[if !IE]> -->
         <div id="jquery-cycle" class="pics">
         <div id="jquery-cycle" class="pics">
Line 49: Line 52:
<img src="https://static.igem.org/mediawiki/2013/f/f1/Mr-igem-sponsors-home.png" alt="Our Sponsors" usemap="#sponsors" />
<img src="https://static.igem.org/mediawiki/2013/f/f1/Mr-igem-sponsors-home.png" alt="Our Sponsors" usemap="#sponsors" />
   <map name="sponsors">
   <map name="sponsors">
-
     <area shape="rect" coords="0,0,375,81"
+
     <area shape="rect" coords="0,0,376,80"
-
           href="http://www.synmikro.com/" alt="Synmikro" title="Synmikro" />
+
           href="http://www.synmikro.com/" target="_blank" alt="Synmikro" title="Synmikro" />
-
     <area shape="rect" coords="390,0,690,81"
+
     <area shape="rect" coords="406,0,686,80"
-
           href="http://www.healthcare.siemens.com/" alt="Siemens" title="Siemens" />
+
           href="http://www.hessen-trade-and-invest.com/" target="_blank" alt="Hessen Trade & Invest" title="Hessen Trade & Invest" />
-
     <area shape="rect" coords="705,0,1000,81"
+
    <area shape="rect" coords="683,0,990,80"
-
          href="http://www.hessen-trade-and-invest.com/" alt="Hessen Trade & Invest" title="Hessen Trade & Invest" />
+
          href="http://www.abbvie.com/" target="_blank" alt="Abbvie" title="Abbvie" />
-
 
+
    <area shape="rect" coords="140,85,420,160"
 +
          href="http://www.healthcare.siemens.com/" target="_blank" alt="Siemens" title="Siemens" />
 +
     <area shape="rect" coords="523,85,841,160"
 +
          href="http://www.erasynbio.eu/" target="_blank" alt="ERA SynBio" title="ERA SynBio" />
</map>
</map>
</html>
</html>

Latest revision as of 18:42, 28 October 2013

The iGEM Marburg proudly presents: PHAECTORY

The use of proteins in medical treatment and diagnosis is steadily increasing. Many of these proteins (e.g. antibodies) have a complex biological structure, which complicates their production. Also, these proteins need to be highly pure. Therefore, a major challenge is the development of systems that produce complex proteins with high purity - best at low costs!

The sunlight-driven microalgae Phaeodactylum tricornutum has been used for the production of complex proteins. A major advantage of the algae is its ability to secrete proteins directly into the medium. This feature would greatly simplify purification of recombinant proteins, and lower production costs! Here we take advantage of the excellent secretion abilities of P. tricornutum, and make this organism accessible to synthetic biology and the iGEM competition (PHAECTORY).

Furthermore, we used a light-inducible promoter which we have characterized quantitatively in order to control protein expression in PHAECTORY (Light control). Also, we introduced a transferable element to the registry, which allows autonomous targeting of proteins to the inner surface of cell membranes (Improve a Brick). We hope you enjoy reading about our project!



Our Sponsors


Our Sponsors Synmikro Hessen Trade & Invest Abbvie Siemens ERA SynBio