- 1 µl Plasmid (pSB1C3-iBB10, pSB1C3-iBB11, pSB1C3-iBB12 and pSB1C3-iBB13)
- 0.5 µl EcoRI
- 0.5 µl SpeI
- 2 µl CutSmart
- 12 µl ddH2O
- 1 µl Plasmid (pSB1C3-iBB9)
- 0.5 µl XbaI
- 0.5 µl PstI
- 2 µl CutSmart
- 12 µl ddH2O
- 1 µl Plasmid (pSB1A3)
- 0.5 µl EcoRI
- 0.5 µl PstI
- 2 µl CutSmart
- 12 µl ddH2O
The samples were incubated for 1.5 h at 37° C.
| Gel electrophoresis |
|
Gel substances
- 1% Agarose gel
- 10 µl RedSafe in 50 ml gel
- x µl Hyper Ladder
Expactations
- Lane 1: 5300 kbp
- Lane 2: 3300 kbp
- Lane 3: 1300 kbp
All expected fragments were present. The relevant fragments were cut from the gel and then purified via “Qiagen Gel Extraction Kit” (Qiagen, Düsseldorf).
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