Team:Marburg/Project:Milestones

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PHAECTORY: Milestones
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PHAECTORY: Milestones <html><a href="https://2013.igem.org/Team:Marburg/Results"><img src="https://static.igem.org/mediawiki/2013/7/71/Mr-igem-next-arrow.png" style="float:right;margin-left:5px !important;" alt="Next"></a>&nbsp;<a href="https://2013.igem.org/Team:Marburg/Project:Ptricornutum"><img src="https://static.igem.org/mediawiki/2013/1/13/Mr-igem-previous-arrow.png" alt="Previous" style="float:right;"></a></html>
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<li class="p1"><div><a class="a1" href="#p1"><b>BioBricks</b></a><img src="https://static.igem.org/mediawiki/2013/4/4b/1_bricks.png" width="569" alt="" title="">
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<span>Construction of specific <i>P. tricornutum</i> <a href="https://2013.igem.org/Team:Marburg/Parts">BioBricks</a>.</span></div></li>
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<li class="p1"><div><a class="a1" href="#p1"><b>Bio Bricks</b></a><img src="https://static.igem.org/mediawiki/2013/4/4b/1_bricks.png" alt="" title="">
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<span>First of all we have to construct our specific <i>P. tricornutum</i> <a href="https://2013.igem.org/Team:Marburg/Parts">bio bricks</a>.</span></div></li>
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<li class="p2"><div><a class="a2" href="#p2"><b>Vector Design</b></a><img src="https://static.igem.org/mediawiki/2013/c/c6/2_vector_2.png" alt="" title="">
<li class="p2"><div><a class="a2" href="#p2"><b>Vector Design</b></a><img src="https://static.igem.org/mediawiki/2013/c/c6/2_vector_2.png" alt="" title="">
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<span>A specific vector is designed</span></div></li>
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<span>Design of <i>P. tricornutum</i> specific vectors.</span></div></li>
<li class="p3"><div><a class="a3" href="#p3"><b>Transfection</b></a><img src="https://static.igem.org/mediawiki/2013/a/af/3_transfection_2.png" alt="" title="">
<li class="p3"><div><a class="a3" href="#p3"><b>Transfection</b></a><img src="https://static.igem.org/mediawiki/2013/a/af/3_transfection_2.png" alt="" title="">
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<span>The vector is transfected into the algae</span></div></li>
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<span>Transfection of vectors into <i>P. tricornutum</i>.</span></div></li>
<li class="p4"><div><a class="a4" href="#p4"><b>PHAECTORY</b></a><img src="https://static.igem.org/mediawiki/2013/6/69/4_phaectory_2.png" alt="" title="">
<li class="p4"><div><a class="a4" href="#p4"><b>PHAECTORY</b></a><img src="https://static.igem.org/mediawiki/2013/6/69/4_phaectory_2.png" alt="" title="">
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<span>The Phaectory only needs sunlight and is CO2-neutral</span></div></li>
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<span>Growth of PHAECTORY needs only sunlight and is CO2-neutral.</span></div></li>
<li class="p5"><div><a class="a5" href="#p5"><b>Products</b></a><img src="https://static.igem.org/mediawiki/2013/8/8c/5_products.png" alt="" title="">
<li class="p5"><div><a class="a5" href="#p5"><b>Products</b></a><img src="https://static.igem.org/mediawiki/2013/8/8c/5_products.png" alt="" title="">
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<span>Antibodies, complex proteins, spider silk, PET</span></div></li>
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<span style="line-height:11pt;">Isolation of complex proteins (e.g. antibodies), which were secreted from PHAECTORY.</span></div></li>
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Here we present a schematic overview on the milestones of our project. Just click on each of the production steps to learn more about our way of building PHAECTORY.
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'''<html><a href="#p1">BioBricks</a></html>''': In a first step standardized BioBricks for <i>Phaeodactylum tricornutum</i> have to be designed in order to produce complex proteins of choice.
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'''<html><a href="#p2">Vector design</a></html>''': Use our provided BioBricks to create your individual expression vector.
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'''<html><a href="#p3">Transfection</a></html>''': With the help of our transfection protocol, it is easy to rapidly integrate novel genetic information into the genome of ''P. tricornutum''.
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'''<html><a href="#p4">PHAECTORY</a></html>''': ''[[Team:Marburg/Project:Ptricornutum|Phaeodactylum tricornutum]]'' can be used for the large-scale production of your protein of choice. PHAECTORY needs therefore only a salt medium or sea water, sunlight and CO2.
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'''<html><a href="#p5">Products</a></html>''': All kinds of complex proteins, bioplastic, spider silk and biofuel can easily be produced with [[Team:Marburg/Project|PHAECTORY]]. Therebye, the high-quality products are directly secreted into the medium.
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Latest revision as of 17:25, 28 October 2013

PHAECTORY: Milestones Next Previous

  • BioBricks Construction of specific P. tricornutum BioBricks.
  • Vector Design Design of P. tricornutum specific vectors.
  • Transfection Transfection of vectors into P. tricornutum.
  • PHAECTORY Growth of PHAECTORY needs only sunlight and is CO2-neutral.
  • Products Isolation of complex proteins (e.g. antibodies), which were secreted from PHAECTORY.

Here we present a schematic overview on the milestones of our project. Just click on each of the production steps to learn more about our way of building PHAECTORY.

BioBricks: In a first step standardized BioBricks for Phaeodactylum tricornutum have to be designed in order to produce complex proteins of choice.

Vector design: Use our provided BioBricks to create your individual expression vector.

Transfection: With the help of our transfection protocol, it is easy to rapidly integrate novel genetic information into the genome of P. tricornutum.

PHAECTORY: Phaeodactylum tricornutum can be used for the large-scale production of your protein of choice. PHAECTORY needs therefore only a salt medium or sea water, sunlight and CO2.

Products: All kinds of complex proteins, bioplastic, spider silk and biofuel can easily be produced with PHAECTORY. Therebye, the high-quality products are directly secreted into the medium.