Team:Paris Saclay/Notebook/August/7

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(1 - Ligation of RBS-LacZ-Term in PSB1C3)
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===='''Objective : obtaining BBa_K1155007'''====
===='''Objective : obtaining BBa_K1155007'''====
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===='''1 - Ligation of RBS-LacZ-Term in PSB1C3 ====
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===='''1 - Ligation of RBS-LacZ-Term in pSB1C3 ====
Anaïs, Nadia
Anaïs, Nadia

Revision as of 17:44, 3 October 2013

Contents

Notebook : August 7

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155007

1 - Ligation of RBS-LacZ-Term in pSB1C3

Anaïs, Nadia

Used quantities :

  • RBS-AmilCP : 2µL
  • Term in PSB1C3 : 2.5µL
  • Buffer : 1µL
  • Ligase : 1µL
  • H2O : 3.5µL

2 - Tranformation BBa_K115507 in DH5α

XiaoJing

Protocol : Bacterial transformation

Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006

1 - Tranduction of Km in MG1655Z1

Nadia, XioaJing

Protocol : Transduction

Our Mutant bacteria was called BW : Δfnr::Km. Our wild type bacteria was called MG1655Z1.

We did the first step of the protocol : bacteriophage stock which packed Km gene.

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