Team:Paris Saclay/Notebook/July/10

From 2013.igem.org

(Difference between revisions)
(1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α)
(1 - Mini and maxi preparation of the culture of Bba_J04450)
Line 31: Line 31:
|}
|}
-
Protocol : [[Team:Paris_Saclay/Protocols/Mini and maxi preparation|Mini and maxi preparation]]
+
Protocol : [[Team:Paris_Saclay/mini_maxi|Mini and maxi preparation]]
We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
-
 
==='''B - PCB sensor system'''===
==='''B - PCB sensor system'''===

Revision as of 18:32, 25 September 2013

Contents

Notebook : July 10

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α

Sheng

Transformation of Bba_B0010 of 07/09/13 didn't work. We will do it again.

Protocol : Bacterial transformation

Objective : obtaining biobricks in PSB3K3

1 - Mini and maxi preparation of the culture of Bba_J04450

Abdou

Transformation of Bba_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.

Protocol : Mini and maxi preparation

We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.

B - PCB sensor system

Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2 protein

1 - Transformation of ligation of PSB1C3 and BphA1 or BphR1 or BphR2

Abdou

Protocol : Bacterial transformation


Previous day Back to calendar Next day