Team:Paris Saclay/Notebook/July/10

From 2013.igem.org

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==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
+
===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
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===='''1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α'''====
+
===='''1 - Transformation of BBa_I732019 in DH5α'''====
Sheng
Sheng
-
 
-
{|
 
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| style="border:1px solid black;padding:5px;background-color:#DE;" |
 
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Transformation of Bba_B0010 of 07/09/13 didn't work. We will do it again.
 
-
|}
 
Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
-
===='''Objective : obtaining biobricks in PSB3K3'''====
+
===='''Objective : obtaining biobricks in pSB3K3'''====
-
===='''1 - Mini and maxi preparation of the culture of Bba_J04450'''====
+
===='''1 - Mini and maxi preparation of the culture of BBa_J04450'''====
Abdou
Abdou
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{|
{|
| style="border:1px solid black;padding:5px;background-color:#DE;" |
| style="border:1px solid black;padding:5px;background-color:#DE;" |
-
Transformation of Bba_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.
+
Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.
|}
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We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
 +
==='''B - PCB sensor system'''===
==='''B - PCB sensor system'''===
-
===='''Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2 protein'''====
+
===='''Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein'''====
-
===='''1 - Transformation of ligation of PSB1C3 and BphA1 or BphR1 or BphR2'''====
+
===='''1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2'''====
Abdou
Abdou
-
Protocol : [[Team:Paris_Saclay/Protocols/Bacterial transformation|Bacterial transformation]]
+
Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]

Latest revision as of 16:36, 4 October 2013

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Contents

Notebook : July 10

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 - Transformation of BBa_I732019 in DH5α

Sheng

Protocol : Bacterial transformation

Objective : obtaining biobricks in pSB3K3

1 - Mini and maxi preparation of the culture of BBa_J04450

Abdou

Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.

Protocol : Mini and maxi preparation

We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.


B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein

1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2

Abdou

Protocol : Bacterial transformation


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