Team:SJTU-BioX-Shanghai/Project

From 2013.igem.org

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(Why Light?)
(Why CRISPRi AND Light?)
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* Uniformly distribute in cell culture.
* Uniformly distribute in cell culture.
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=<font color=white>Why CRISPRi AND Light?</font>=
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=<font color=white>Why CRISPRi AND Lights?</font>=
* Light alone? CANNOT be easily applied on genomic genes.
* Light alone? CANNOT be easily applied on genomic genes.

Revision as of 21:10, 18 October 2013


Project Overview

13SJTU overview.png

Why CRISPRi?

  • Regulate genomic genes without changing their promoters.
  • "Totipotent"! Change the target gene as you want by making some minor changes in gRNA sequence.

Why Lights?

  • Easy to adjust! Get rid of those laborious work of adding inducing chemicals! :)
  • Multi-control! lights of different colors can be exerted simultaneously. And one sensor will only be activated by photons with a narrow range of wavelengths.
  • Accurate!
  • Uniformly distribute in cell culture.

Why CRISPRi AND Lights?

  • Light alone? CANNOT be easily applied on genomic genes.
  • CRISPRi alone? HARD to be simultaneous; NOT convenient.

Why Computer?

Computer and Electronics! Experience the information century!

  • Input your desired expression level into the computer. And just leave the rest to our program and illuminating device to adjust to your specific requirement.