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Team:TU-Delft/Notebook/2013/09/02/
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1. Made glycerol stock of: <br> | 1. Made glycerol stock of: <br> | ||
| - | pT7 SUMO Maximin H5 pSB1C3 <br> | + | pT7 SUMO Maximin H5 pSB1C3 <br> |
| - | pT7 SUMO Signiferin pSB1C3<br> | + | pT7 SUMO Signiferin pSB1C3<br> |
| - | pT7 SUMO Magainin II pSB1C3<br> | + | pT7 SUMO Magainin II pSB1C3<br> |
| - | pT7 SUMO Maximin H5 Tet R (Bharat) <br> | + | pT7 SUMO Maximin H5 Tet R (Bharat) <br> |
| - | pT7 SUMO Signiferin Tet R (Bharat) <br> | + | pT7 SUMO Signiferin Tet R (Bharat) <br> |
| - | pT7 SUMO Magainin II Tet R (Bharat)<br> | + | pT7 SUMO Magainin II Tet R (Bharat)<br> |
| - | pT7 SUMO Maximin H5 Tet R (Derk)<br> | + | pT7 SUMO Maximin H5 Tet R (Derk)<br> |
| - | pT7 SUMO Signiferin Tet R (Derk)<br> | + | pT7 SUMO Signiferin Tet R (Derk)<br> |
| - | pT7 SUMO Magainin II Tet R (Derk)<br> | + | pT7 SUMO Magainin II Tet R (Derk)<br> |
| - | pBAD Receiver Impure<br> | + | pBAD Receiver Impure<br> |
| - | pBAD Receiver Pure <br> | + | pBAD Receiver Pure <br> |
| - | pcI:Ulp <br> | + | pcI:Ulp <br> |
<br> | <br> | ||
2. Did Colony PCR on the following: <br> | 2. Did Colony PCR on the following: <br> | ||
| - | pT7 SUMO Maximin H5 pSB1C3 <br> | + | pT7 SUMO Maximin H5 pSB1C3 <br> |
| - | pT7 SUMO Signiferin pSB1C3<br> | + | pT7 SUMO Signiferin pSB1C3<br> |
| - | pT7 SUMO Magainin II pSB1C3<br> | + | pT7 SUMO Magainin II pSB1C3<br> |
| - | pT7 SUMO Maximin H5 Tet R (Bharat) <br> | + | pT7 SUMO Maximin H5 Tet R (Bharat) <br> |
| - | pT7 SUMO Signiferin Tet R (Bharat) <br> | + | pT7 SUMO Signiferin Tet R (Bharat) <br> |
| - | pT7 SUMO Magainin II Tet R (Bharat)<br> | + | pT7 SUMO Magainin II Tet R (Bharat)<br> |
| - | pT7 SUMO Maximin H5 Tet R (Derk)<br> | + | pT7 SUMO Maximin H5 Tet R (Derk)<br> |
| - | pT7 SUMO Signiferin Tet R (Derk)<br> | + | pT7 SUMO Signiferin Tet R (Derk)<br> |
| - | pT7 SUMO Magainin II Tet R (Derk)<br> | + | pT7 SUMO Magainin II Tet R (Derk)<br> |
| - | pBAD Receiver Impure<br> | + | pBAD Receiver Impure<br> |
| - | pBAD Receiver Pure <br><br> | + | pBAD Receiver Pure <br><br> |
3. Did Mini prep on the following : <br> | 3. Did Mini prep on the following : <br> | ||
| - | pT7 (BBa_I712074) <br> | + | pT7 (BBa_I712074) <br> |
| - | Lysis Device <br> <br> | + | Lysis Device <br> <br> |
4. Restriction digestion was carried out on: <br> | 4. Restriction digestion was carried out on: <br> | ||
| - | Lysis device with E+X, X+P <br> | + | Lysis device with E+X, X+P <br> |
| - | pcI:Ulp: with E+S <br> | + | pcI:Ulp: with E+S <br> |
| - | pT7 with S+P <br> | + | pT7 with S+P <br> |
| - | pTET:cI:TT with E+S <br> | + | pTET:cI:TT with E+S <br> |
Latest revision as of 09:02, 4 October 2013
Notebook
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| 29 | 30 |
2nd of September
Lab work
1. Made glycerol stock of:
pT7 SUMO Maximin H5 pSB1C3
pT7 SUMO Signiferin pSB1C3
pT7 SUMO Magainin II pSB1C3
pT7 SUMO Maximin H5 Tet R (Bharat)
pT7 SUMO Signiferin Tet R (Bharat)
pT7 SUMO Magainin II Tet R (Bharat)
pT7 SUMO Maximin H5 Tet R (Derk)
pT7 SUMO Signiferin Tet R (Derk)
pT7 SUMO Magainin II Tet R (Derk)
pBAD Receiver Impure
pBAD Receiver Pure
pcI:Ulp
2. Did Colony PCR on the following:
pT7 SUMO Maximin H5 pSB1C3
pT7 SUMO Signiferin pSB1C3
pT7 SUMO Magainin II pSB1C3
pT7 SUMO Maximin H5 Tet R (Bharat)
pT7 SUMO Signiferin Tet R (Bharat)
pT7 SUMO Magainin II Tet R (Bharat)
pT7 SUMO Maximin H5 Tet R (Derk)
pT7 SUMO Signiferin Tet R (Derk)
pT7 SUMO Magainin II Tet R (Derk)
pBAD Receiver Impure
pBAD Receiver Pure
3. Did Mini prep on the following :
pT7 (BBa_I712074)
Lysis Device
4. Restriction digestion was carried out on:
Lysis device with E+X, X+P
pcI:Ulp: with E+S
pT7 with S+P
pTET:cI:TT with E+S
