Team:TU-Delft/Notebook/2013/09/02/

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Notebook

2nd of September

Lab work

1. Made glycerol stock of:
pT7 SUMO Maximin H5 pSB1C3
pT7 SUMO Signiferin pSB1C3
pT7 SUMO Magainin II pSB1C3
pT7 SUMO Maximin H5 Tet R (Bharat)
pT7 SUMO Signiferin Tet R (Bharat)
pT7 SUMO Magainin II Tet R (Bharat)
pT7 SUMO Maximin H5 Tet R (Derk)
pT7 SUMO Signiferin Tet R (Derk)
pT7 SUMO Magainin II Tet R (Derk)
pBAD Receiver Impure
pBAD Receiver Pure
pcI:Ulp

2. Did Colony PCR on the following:
pT7 SUMO Maximin H5 pSB1C3
pT7 SUMO Signiferin pSB1C3
pT7 SUMO Magainin II pSB1C3
pT7 SUMO Maximin H5 Tet R (Bharat)
pT7 SUMO Signiferin Tet R (Bharat)
pT7 SUMO Magainin II Tet R (Bharat)
pT7 SUMO Maximin H5 Tet R (Derk)
pT7 SUMO Signiferin Tet R (Derk)
pT7 SUMO Magainin II Tet R (Derk)
pBAD Receiver Impure
pBAD Receiver Pure

3. Did Mini prep on the following :
pT7 (BBa_I712074)
Lysis Device

4. Restriction digestion was carried out on:
Lysis device with E+X, X+P
pcI:Ulp: with E+S
pT7 with S+P
pTET:cI:TT with E+S

Sun	Mon	Tue	Wed	Thu	Fri	Sat
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2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30

Sun	Mon	Tue	Wed	Thu	Fri	Sat
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30	31

Sun	Mon	Tue	Wed	Thu	Fri	Sat
				1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	31

Sun	Mon	Tue	Wed	Thu	Fri	Sat
1	2	3	4	5	6	7
8	9	10	11	12	13	14
15	16	17	18	19	20	21
22	23	24	25	26	27	28
29	30