Team:UANL Mty-Mexico/Results/Protocols
From 2013.igem.org
(Difference between revisions)
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- | <li | + | <li class="glyphicon glyphicon-expand">Preparation of <i>E</i>. <i>coli</i> Calcium Chloride competent cells<ul> |
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<li>Inoculate a single colony into 5 mL of LB media without any antibiotics and grow overnight at 37 °C with vigorous shaking.</li> | <li>Inoculate a single colony into 5 mL of LB media without any antibiotics and grow overnight at 37 °C with vigorous shaking.</li> | ||
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- | <li | + | <li class="glyphicon glyphicon-expand">Preparation of <i>E</i>. <i>coli</i> Rubidium Chloride competent cells<ul> |
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<li>Inoculate 5 mL of LB broth with DH5α and incubate the culture overnight at 37°C with vigorous shacking.</li> | <li>Inoculate 5 mL of LB broth with DH5α and incubate the culture overnight at 37°C with vigorous shacking.</li> | ||
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- | <li | + | <li class="glyphicon glyphicon-expand">Heat-shock transformation of <i>E</i>. <i>coli</i> competent cells |
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<ol> | <ol> | ||
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- | <li | + | <li class="glyphicon glyphicon-expand">Preparation of Electrocompetent <i>E</i>. <i>coli</i> cells</b> |
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- | <li | + | <li class="glyphicon glyphicon-expand">Electroporation of <i>E</i>. <i>coli</i> competent cells |
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- | <li | + | <li class="glyphicon glyphicon-expand">Mini preparation of plasmid DNA<ul> |
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- | <li | + | <li class="glyphicon glyphicon-expand">Midi preparation of plasmid DNA (AXYPREP ™ PLASMID MIDIPREP KIT)<ul> |
<ol> | <ol> | ||
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- | <li | + | <li class="glyphicon glyphicon-expand">Solutions for Mini preparation of Plasmid DNA<ul> |
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- | <li | + | <li class="glyphicon glyphicon-expand">Agarose Gel Electrophoresis Protocol<ul> |
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- | <li | + | <li class="glyphicon glyphicon-expand">Agarose gel<ul> |
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- | <li | + | <li class="glyphicon glyphicon-expand">SB buffer 20X |
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<br> | <br> | ||
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- | <li | + | <li class="glyphicon glyphicon-expand">Ethidium Bromide Gel Staining |
<ul><span> | <ul><span> | ||
<ol> | <ol> | ||
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- | <li | + | <li class="glyphicon glyphicon-expand">Lambda/PstI Molecular Size Marker |
<ul><span> | <ul><span> | ||
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- | <li | + | <li class="glyphicon glyphicon-expand">Restriction enzyme digestion of DNA<ul> |
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- | <li | + | <li class="glyphicon glyphicon-expand">PCR |
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</span></ul></li> | </span></ul></li> | ||
- | <li | + | <li class="glyphicon glyphicon-expand"><a name="Antibiotics"></a>Antibiotics |
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Revision as of 20:39, 7 September 2013