Rehydration of Registry DNA

Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.

Reagents and Reagents

• Biobrick parts
• ddH₂O (deionized water)

Protocol

1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
2. Add 10 μL of ddH₂O (deionized water)
3. Let the water incubate at room temperature for 10 minutes
4. Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies