"
Team:Heidelberg/Delftibactin/DelH
From 2013.igem.org
Nils.kurzawa (Talk | contribs) m |
Nils.kurzawa (Talk | contribs) m |
||
| Line 61: | Line 61: | ||
<h1>Week 1</h1> | <h1>Week 1</h1> | ||
| - | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;"> | + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;"> |
| + | In order to transfer the gold precipitating NRPS from <i>D. acidovorans</i> to <i>E.coli</i>, the necessary modules will be amplified from the D. acidovorans genome and assembled as plasmids. Due to its large size of 18 Kb, the module DelH will be expressed on a separate plasmid. A strategy was developed, primers designed accordingly and necessary BioBricks obtained from the distribution. The <i>D. acidovorans</i> was obtained from the DSMZ and cultured in Acidovorax complex medium. | ||
| + | </p> | ||
</div> | </div> | ||
| Line 69: | Line 71: | ||
<div class="container"> | <div class="container"> | ||
<div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| - | <h1>Week | + | <h1>Week 2</h1> |
<p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
<p><a class="btn btn-large btn-primary" href="#">Learn more</a></p> | <p><a class="btn btn-large btn-primary" href="#">Learn more</a></p> | ||
| Line 80: | Line 82: | ||
<div class="container"> | <div class="container"> | ||
<div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| - | <h1>Week | + | <h1>Week 3</h1> |
| + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
| + | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | ||
| + | <p>At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed. After the Transformation to DH10β cells and screening by restriction digest we could send samples for the Dipeptide and Tetrapeptide I to sequencing and obtained a positive alignment. Hence we transformed BAP I cells with the positive constructs. The same was... | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="item may"> | ||
| + | <div class="container"> | ||
| + | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| + | <h1>Week 4</h1> | ||
| + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
| + | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="item may last"> | ||
| + | <div class="container"> | ||
| + | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| + | <h1>Week 5</h1> | ||
| + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed.</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="item june first"> | ||
| + | <img src="data:image/png;base64,"/> | ||
| + | <div class="container"> | ||
| + | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| + | <h1>Week 6</h1> | ||
| + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
| + | <p><a class="btn btn-large btn-primary" href="#">Learn more</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="item june"> | ||
| + | <div class="container"> | ||
| + | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| + | <h1>Week 7</h1> | ||
| + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
| + | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="item june"> | ||
| + | <img src="data:image/png;base64,"/> | ||
| + | <div class="container"> | ||
| + | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| + | <h1>Week 8</h1> | ||
| + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
| + | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="item june last"> | ||
| + | <div class="container"> | ||
| + | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0" id="week1"> | ||
| + | <h1>Week 9</h1> | ||
| + | |||
| + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;"> | ||
| + | In order to transfer the gold precipitating NRPS from <i>D. acidovorans</i> to <i>E.coli</i>, the necessary modules will be amplified from the D. acidovorans genome and assembled as plasmids. Due to its large size of 18 Kb, the module DelH will be expressed on a separate plasmid. A strategy was developed, primers designed accordingly and necessary BioBricks obtained from the distribution. The <i>D. acidovorans</i> was obtained from the DSMZ and cultured in Acidovorax complex medium. | ||
| + | </p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="item july first"> | ||
| + | <div class="container"> | ||
| + | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| + | <h1>Week 10</h1> | ||
| + | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
| + | <p><a class="btn btn-large btn-primary" href="#">Learn more</a></p> | ||
| + | <p>At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed. After the Transformation to DH10β cells and screening by restriction digest we could send samples for the Dipeptide and Tetrapeptide I to sequencing and obtained a positive alignment. Hence we transformed BAP I cells with the positive constructs. The same was... | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="item may"> | ||
| + | <div class="container"> | ||
| + | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| + | <h1>Week 11</h1> | ||
<p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
<p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | ||
| Line 91: | Line 173: | ||
<div class="container"> | <div class="container"> | ||
<div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| - | <h1>Week | + | <h1>Week 12</h1> |
<p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
<p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | ||
| Line 100: | Line 182: | ||
<div class="container"> | <div class="container"> | ||
<div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| - | <h1>Week | + | <h1>Week 13</h1> |
<p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed.</p> | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed.</p> | ||
</div> | </div> | ||
| Line 109: | Line 191: | ||
<div class="container"> | <div class="container"> | ||
<div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| - | <h1>Week | + | <h1>Week 14</h1> |
<p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
<p><a class="btn btn-large btn-primary" href="#">Learn more</a></p> | <p><a class="btn btn-large btn-primary" href="#">Learn more</a></p> | ||
| Line 118: | Line 200: | ||
<div class="container"> | <div class="container"> | ||
<div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| - | <h1>Week | + | <h1>Week 15</h1> |
<p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
<p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | ||
| Line 128: | Line 210: | ||
<div class="container"> | <div class="container"> | ||
<div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0"> | ||
| - | <h1>Week | + | <h1>Week 16</h1> |
<p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p> | ||
<p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p> | ||
Revision as of 14:03, 1 October 2013
Project
Notebook
Human Practice
Del H. This bitchy 18 kbp fragment.
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.
Week 1
In order to transfer the gold precipitating NRPS from D. acidovorans to E.coli, the necessary modules will be amplified from the D. acidovorans genome and assembled as plasmids. Due to its large size of 18 Kb, the module DelH will be expressed on a separate plasmid. A strategy was developed, primers designed accordingly and necessary BioBricks obtained from the distribution. The D. acidovorans was obtained from the DSMZ and cultured in Acidovorax complex medium.
Week 2
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed. After the Transformation to DH10β cells and screening by restriction digest we could send samples for the Dipeptide and Tetrapeptide I to sequencing and obtained a positive alignment. Hence we transformed BAP I cells with the positive constructs. The same was...
Week 3
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed. After the Transformation to DH10β cells and screening by restriction digest we could send samples for the Dipeptide and Tetrapeptide I to sequencing and obtained a positive alignment. Hence we transformed BAP I cells with the positive constructs. The same was...
Week 4
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
Week 5
At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed.
Week 6
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
Week 7
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
Week 8
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
Week 9
In order to transfer the gold precipitating NRPS from D. acidovorans to E.coli, the necessary modules will be amplified from the D. acidovorans genome and assembled as plasmids. Due to its large size of 18 Kb, the module DelH will be expressed on a separate plasmid. A strategy was developed, primers designed accordingly and necessary BioBricks obtained from the distribution. The D. acidovorans was obtained from the DSMZ and cultured in Acidovorax complex medium.
Week 10
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed. After the Transformation to DH10β cells and screening by restriction digest we could send samples for the Dipeptide and Tetrapeptide I to sequencing and obtained a positive alignment. Hence we transformed BAP I cells with the positive constructs. The same was...
Week 11
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed. After the Transformation to DH10β cells and screening by restriction digest we could send samples for the Dipeptide and Tetrapeptide I to sequencing and obtained a positive alignment. Hence we transformed BAP I cells with the positive constructs. The same was...
Week 12
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
Week 13
At the beginning of the week, we could verify that the Gibson Assembly for Tripeptide I was indeed positive, however, the other Gibson Assemblies did not work properly. Instead of picking new colonies, we decided to optimize the Gibson recipe instead, as backbone religations were the most common problem. With these improved protocols, we used Gibson Assembly for the Dipeptide, Tripeptide II and Tetrapeptide I, later that week, Tetrapeptide II followed.
Week 14
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
Week 15
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
Week 16
Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.
Methods:
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. .
