Team:Paris Saclay/Notebook/June/24
From 2013.igem.org
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- | - Order | + | - Order oligonucleotides and strains |
- | - Research the intergenic sequence which contain the promoter for | + | - Research the intergenic sequence which contain the promoter for ''Pseudomonas pseudoalcaligenes'' KF 707 |
- | - Research on | + | - Research on ''Rhodococcus rhodoccrous'' RHA1 |
- Research on Registry of standard biological parts | - Research on Registry of standard biological parts | ||
Line 17: | Line 17: | ||
- Code: BBa_ I722017. We don’t get it in the kit therefore we could ask to iGEM. | - Code: BBa_ I722017. We don’t get it in the kit therefore we could ask to iGEM. | ||
- | - | + | - Design the Pndh* promoter primers called pfrn_ up and pfnr_down for forward and reverse primer, respectively |
- | - | + | - List the BioBricks which we may have a need |
- | - | + | - Plan the first part manipulation (the first and second BBa) |
- | - Control and | + | - Control and verify the construction if we have some BioBrick with RBS and Terminators that could be used after |
- | - General presentation about PCBs for Wiki | + | - General presentation written in Word about PCBs for Wiki |
- | - 3 ml | + | - Prepare 3 ml overnight culture of E.Coli DH5α in LB medium |
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+ | |||
+ | {| border="1" align="center" | ||
+ | |[[Team:Paris Saclay/Notebook/June/21|<big>Previous day</big>]] | ||
+ | |||
+ | |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]] | ||
+ | |||
+ | |[[Team:Paris Saclay/Notebook/June/25|<big>Next day</big>]] | ||
+ | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} |
Latest revision as of 00:22, 5 October 2013
Notebook : June 24
Lab work
- Order oligonucleotides and strains
- Research the intergenic sequence which contain the promoter for Pseudomonas pseudoalcaligenes KF 707
- Research on Rhodococcus rhodoccrous RHA1
- Research on Registry of standard biological parts
- Search and document about the BioBrick which contain the lacZ with RBS and terminator
- Code: BBa_ I722017. We don’t get it in the kit therefore we could ask to iGEM.
- Design the Pndh* promoter primers called pfrn_ up and pfnr_down for forward and reverse primer, respectively
- List the BioBricks which we may have a need
- Plan the first part manipulation (the first and second BBa)
- Control and verify the construction if we have some BioBrick with RBS and Terminators that could be used after
- General presentation written in Word about PCBs for Wiki
- Prepare 3 ml overnight culture of E.Coli DH5α in LB medium
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