Hydrolase constructs |
|
Description |
Cloning |
Maps |
14 |
Aes coding region with RBS in SB1C3 backbone |
[http://parts.igem.org/Part:BBa_K1216002 BBa_K1216002] was built through touchdown PCR amplification. Biobrick prefix and suffix were added with overhangs. Primers: 5'-gtttcttcgaattcgcggccgcttctagaggtttaactttaagaaggagatatagctagc 5'-gtttcttcctgcagcggccgctactagtattattaaagctgagcggtaaagaactgag |
![Pla14.png](/wiki/images/a/a6/Pla14.png) |
15 |
Aes-His coding region with RBS in SB1C3 backbone |
[http://parts.igem.org/Part:BBa_K1216006 BBa_K1216006] was built through touchdown PCR amplification. Biobrick prefix and suffix were added with overhangs. Primers: 5'-gtttcttcgaattcgcggccgcttctagaggtttaactttaagaaggagatatagctagc
5'-gtttcttcctgcagcggccgctactagtattattagtggtggtggtggtggtgctc |
![Pla15.png](/wiki/images/a/a0/Pla15.png) |
16 |
GusA coding region with RBS in SB1C3 backbone |
[http://parts.igem.org/Part:BBa_K1216000 BBa_K1216000] was built through touchdown PCR amplification. Biobrick prefix and suffix were added with overhangs. Primers: 5'-gtttcttcgaattcgcggccgcttctagagtttgtttaactttaagaaggagatatacat 5'-gtttcttcctgcagcggccgctactagtattattattgtttgcctccctgctgcg |
![Pla16.png](/wiki/images/1/11/Pla16.png) |
17 |
GusA-His coding region with RBS in SB1C3 backbone |
[http://parts.igem.org/Part:BBa_K1216004 BBa_K1216004] was built through touchdown PCR amplification. Biobrick prefix and suffix were added with overhangs. Primers: 5'-gtttcttcgaattcgcggccgcttctagagtttgtttaactttaagaaggagatatacat 5'-gtttcttcctgcagcggccgctactagtattattagtggtggtggtggtggtgctc |
![Pla17.png](/wiki/images/d/d5/Pla17.png) |
18 |
PhoA coding region with RBS in SB1C3 backbone |
[http://parts.igem.org/Part:BBa_K1216001 BBa_K1216001] was built through touchdown PCR amplification. Biobrick prefix and suffix were added with overhangs. Primers: 5'-gtttcttcgaattcgcggccgcttctagagtttgtttaactttaagaaggagatatacat 5'-gtttcttcctgcagcggccgctactagtattattacttcaggcccagcgccgctttc |
![Pla18.png](/wiki/images/b/be/Pla18.png) |
19 |
PhoA-His coding region with RBS in SB1C3 backbone |
[http://parts.igem.org/Part:BBa_K1216005 BBa_K1216005] was built through touchdown PCR amplification. Biobrick prefix and suffix were added with overhangs. Primers: 5'-gtttcttcgaattcgcggccgcttctagagtttgtttaactttaagaaggagatatacat 5'-gtttcttcctgcagcggccgctactagtattattagtggtggtggtggtggtgctc |
![Pla19.png](/wiki/images/8/82/Pla19.png) |
20 |
NagZ coding region with RBS in SB1C3 backbone |
[http://parts.igem.org/Part:BBa_K1216003 BBa_K1216003] was built through touchdown PCR amplification. Biobrick prefix and suffix were added with overhangs. Primers: 5'-gtttcttcgaattcgcggccgcttctagaggtttaactttaagaaggagatatagctagc 5'-gtttcttcctgcagcggccgctactagtattattactcgtgacctgctttctcttc |
![Pla20.png](/wiki/images/4/4b/Pla20.png) |
21 |
constitutive expression of PhoA for non-mine cells |
constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] (SpeI, PstI) backbone and [http://parts.igem.org/Part:BBa_K1216001 BBa_K1216001]insert (XbaI, PstI) |
![Pla21.png](/wiki/images/b/b2/Pla21.png)
![Pla22.png](/wiki/images/3/3c/Pla22.png) |
22 |
constitutive expression of NagZ for mine cells |
constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] (SpeI, PstI) backbone and [http://parts.igem.org/Part:BBa_K1216003 BBa_K1216003]insert (XbaI, PstI) |
![Pla23.png](/wiki/images/0/0c/Pla23.png)
![Pla24.png](/wiki/images/c/c1/Pla24.png) |
23 |
OHHL inducible expression of GusA |
[http://parts.igem.org/Part:BBa_J09855 BBa_J09855] (SpeI, PstI) backbone and [http://parts.igem.org/Part:BBa_K1216000 BBa_K1216000]insert (XbaI, PstI) |
![Pla25.png](/wiki/images/3/3f/Pla25.png)
![Pla26.png](/wiki/images/8/8f/Pla26.png) |
24 |
OHHL inducible expression of GusA with positive feedback loop for LuxR expression |
BBa_J09855.BBa_K1216000 construct where the pLac promoter was replaced with pLuxR to build a positive feedback loop. The promoter was inserted with two pairs of custom-made oligos using XbaI and HindIII restriction sites. Oligos: 5’-ctagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactaga
5’-gattaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaa 5’-aatctctagtatttattcgactataacaaaccattttcttgcgtaaacctgtacgatcctacaggtct 5’-agctttaattttattaattattctgtatgtgtcgtcggcatttatgtttttcatctagtatttctcctcttt |
![Pla27.png](/wiki/images/c/c7/Pla27.png)
![Pla28.png](/wiki/images/4/45/Pla28.png) |
25 |
OHHL inducible expression of Aes |
[http://parts.igem.org/Part:BBa_J09855 BBa_J09855] (SpeI, PstI) backbone and [http://parts.igem.org/Part:BBa_K1216002 BBa_K1216002]insert (XbaI, PstI) |
![Pla29.png](/wiki/images/b/b6/Pla29.png) |
26 |
OHHL inducible expression of Aes with mutant pLux promoter |
Library of mutated pLux promoters (see above) backbone (SpeI,PstI) and [http://parts.igem.org/Part:BBa_K1216002 BBa_K1216002] (SpeI,PstI) insert |
![Pla30.png](/wiki/images/b/b6/Pla30.png) |
In the following table you can find all the biobricks that were submitted by our group.