Team:Heidelberg/Parts

From 2013.igem.org

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Revision as of 16:08, 26 October 2013

Our Parts. Fascinating stuff out of the world of non-ribosomal peptides.

Team Parts

Our favorite part is BBa_K1152007: Helper construct for NRP-Indigoidine-tagging.
  • Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
  • Compatibility with custom non-ribosomal peptide (NRP) synthesis.
  • Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.

    We propose BBa_K1152013 as Best new BioBrick Part, Natural, because:
  • it encodes the original NRPS module from Photorhabdus luminescens capable of synthesizing Indigoidine
  • the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
  • standardization and established protocols allow for BioBrick assembly or Gibson assembly

    We propose BBa_K1152007 as Best new BioBrick Part or Device, Engineered, as mentioned above.

    We propose BBa_K1152009 as Most Improved Registry Part, because:
  • it encodes for sfp, a 4'-Phosphopanthetheinyl-transferase (PPTase) crucial for the activation of T domains
  • it improved the parts BBa_K802006 and BBa_K302010, which do also encode for sfp from Bacillus subtilis but were deviating in three amino acids from the sequence published on NCBI
  • it is the first BioBrick in the registry that is explicitly encoding sfp, and it is annotated and characterized

    We propose BBa_K1152015, BBa_K1152016, BBa_K1152017, BBa_K1152018, BBa_K1152019, BBa_K1152020 as Best Part Collection, because:
  • they represent a valuable ensemble of various T domains with different properties
  • they were designed semi-rationally based on multiple sequence alignments
  • they exhibit distinct synthesis rates for Indigoidine depending on the T domains of this part collection

    • List of all submitted parts:

    Thanks to

    Retrieved from "http://2013.igem.org/Team:Heidelberg/Parts"