Since the Del Cluster genes of D. acidovorans, which are considered to be crucial for the production of delftibactin consist of more than 58 kbp, we had to think of a method capable of trasferring this huge amount of genetic information to our host organism E. coli. Being confronted with this task, we considered the usage of so called "Bacterial Artificial Chromosomes (BACs)" or "Cosmids. But finally, we decided to face the challege by splitting up the crucial genes of the cluster into convetional plasmids. One containing the gigantic gene "DelH", which comprises 18 kbp and another containing all residual parts, herein referred to as "DelRest". Additionally, we planned to integrate D. acidovorans's methyl-malonyl-CoA-pathway, which is a crucial co-factor for one of the PKS domains and hence, for successful delftibactin production. Because we were unfortunately not able to clone DelH and thus the entire pathway after several attempts, we decided to create a third plasmid not only containing the pathway, but as well a permeability device for enabeling our bacteria to secrete the desired product. These three plasmids were constructed in different subgroups and therefore have separated lab journals. Follow the links below to discover more!