Team:Heidelberg/Parts

From 2013.igem.org

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COMING SOON!
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[[File:Heidelberg_Team_Logo_Text.png|500px|center]]
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
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Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
 
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<groupparts>iGEM013 Heidelberg</groupparts>
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<h1><span style="font-size:150%; color:#666666">Our Parts.</span><span style="font-size:90%" class="text-muted"> Now it is Your turn. Start working with NRPS!</span></h1>
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                  <h2><span style="font-size:170%;">Synthetic Peptides</span style="font-size:170%;"> - <span style="font-size":50%">BBa_K1152000 - 2005</h2>
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                            We provide the constructs for the novel Dipeptide- and Tripeptide-Synthetases. Furthermore, we submitted the single modules that they are comprised of. These parts should serve as the basis of a future library for standardized work with NRPS. The modules are specific for different amino acids: Phenylalanine (tycA), Proline (tycB1) and Leucine (tycC6).
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                                    <img src="https://static.igem.org/mediawiki/2013/2/26/Heidelberg_Parts_Gallery_1.png" style="width:70%; padding:1%" />
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                                    </center>
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                                </div>
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                  <h2><span style="font-size:170%;">Indigoidine-Tag</span style="font-size:170%;"> - <span style="font-size":50%">BBa_K1152006 & 2007</h2>
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                            One of our major achievements is the establishment of an easily detectable, inert and universal tag - the <a href="https://2013.igem.org/Team:Heidelberg/Project/Indigoidine-Tag">Indigoidine-Tag</a>. With this tag, purification and validation of novel NRPs is significantly eased. The characteristics of the tag compare to those of the GFP-tag for proteins. We submit the helper-construct for tagging any desired Non-Ribosomal Peptide as our <a href="https://2013.igem.org/Team:Heidelberg/Favorite_Parts">favorite part</a>.
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                                </div>
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                                    <img src="https://static.igem.org/mediawiki/2013/e/ef/Heidelberg_ind_slider_6.png" style="width:70%; padding:1%" />
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                                    </center>
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                  <h2><span style="font-size:170%;">Tag-Optimization</span style="font-size:170%;"> - <span style="font-size":50%">BBa_K1152008 & 2013-2019</h2>
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                            Besides establishing the <a href="https://2013.igem.org/Team:Heidelberg/Project/Indigoidine-Tag">Indigoidine-Tag</a>, we focussed on <a href="https://2013.igem.org/Team:Heidelberg/Project/Tag-Optimization">optimizing</a> its functionality and efficiency. Therefore, we modified the natural sequence of the Indigoidine synthetase indC (which is our <a href="https://2013.igem.org/Team:Heidelberg/Favorite_Parts">Best Natural BioBrick</a> by domain exchange which lead to altered and in some cases enhanced efficiency of the NRP-production. We submit the collection of alternate T-domains, of which we created several functional, synthetic ones as <a href="https://2013.igem.org/Team:Heidelberg/Favorite_Parts">Best Part Range</a>.
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                                </div>
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                                    <img src="https://static.igem.org/mediawiki/2013/7/75/Slider_plattentabelle.png" style="width:70%; padding:1%" />
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                                    </center>
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                  <h2><span style="font-size:170%;">Tag-Characterization</span style="font-size:170%;"> - <span style="font-size":50%">BBa_K1152009 - 2012</h2>
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                            During <a href="https://2013.igem.org/Team:Heidelberg/Project/Tag-Optimization">Tag-Optimization</a>, we also focussed on the enzymes that are required for the activation of NRPSs - the PPTases. We characterized several PPTases and assessed their compatibility and efficiency. We thereby improved parts <b><a href="http://parts.igem.org/Part:BBa_K802006">BBa_K802006</a></b> and <b><a href="http://parts.igem.org/Part:BBa_K302010">BBa_K302010</a></b> from the registry, which are PPTase-encoding parts not annotated or characterized.
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<h2><span style="font-size:170%;">List of Parts</span style="font-size:170%;"> - <span style="font-size":50%">BBa_K1152000 - 2019</h2>
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    jQuery("#partsTable").load("https://2013.igem.org/cgi/api/groupparts.cgi?t=iGEM013&amp;g=Heidelberg", function(){
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Latest revision as of 21:57, 28 October 2013

Our Parts. Now it is Your turn. Start working with NRPS!


Synthetic Peptides - BBa_K1152000 - 2005

We provide the constructs for the novel Dipeptide- and Tripeptide-Synthetases. Furthermore, we submitted the single modules that they are comprised of. These parts should serve as the basis of a future library for standardized work with NRPS. The modules are specific for different amino acids: Phenylalanine (tycA), Proline (tycB1) and Leucine (tycC6).



Indigoidine-Tag - BBa_K1152006 & 2007

One of our major achievements is the establishment of an easily detectable, inert and universal tag - the Indigoidine-Tag. With this tag, purification and validation of novel NRPs is significantly eased. The characteristics of the tag compare to those of the GFP-tag for proteins. We submit the helper-construct for tagging any desired Non-Ribosomal Peptide as our favorite part.



Tag-Optimization - BBa_K1152008 & 2013-2019

Besides establishing the Indigoidine-Tag, we focussed on optimizing its functionality and efficiency. Therefore, we modified the natural sequence of the Indigoidine synthetase indC (which is our Best Natural BioBrick by domain exchange which lead to altered and in some cases enhanced efficiency of the NRP-production. We submit the collection of alternate T-domains, of which we created several functional, synthetic ones as Best Part Range.



Tag-Characterization - BBa_K1152009 - 2012

During Tag-Optimization, we also focussed on the enzymes that are required for the activation of NRPSs - the PPTases. We characterized several PPTases and assessed their compatibility and efficiency. We thereby improved parts BBa_K802006 and BBa_K302010 from the registry, which are PPTase-encoding parts not annotated or characterized.




List of Parts - BBa_K1152000 - 2019

Thanks to

Retrieved from "http://2013.igem.org/Team:Heidelberg/Parts"