Team:ETH Zurich/Experiments

From 2013.igem.org

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<b>Tranformation and cloning constructs</b>
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<table style="">
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<tr>
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<th>Sender cells</th>
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<th colspan="2">Receiver cells</th>
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</tr>
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<tr>
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<th>AHL expressing system </th>
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<th>Fluorescent reporters system</th>
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<th>Enzyme reaction-based reporter system</th>
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</tr>
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<tr>
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<td>Pcons-LuxI 1 (Strong)</td>
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<td>Plac-LuxR-Plux-GFP</td>
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<td>Plac-LuxR-Plux-LacZ</td>
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</tr>
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<tr>
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<td>Pcons-LuxI 2 </td>
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<td>Plac-LuxR-Plux-YFP</td>
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<td>Plac-LuxR-Plux-Aes</td>
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</tr>
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<tr>
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<td>Pcons-LuxI 3 </td>
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<td>Plac-LuxR-Plux-CFP</td>
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<td>Plac-LuxR-Plux-GusA</td>
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</tr>
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<tr>
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<td>Pcons-LuxI 4 (Weak) </td>
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<td></td>
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<td>Plac-LuxR-Plux-PhoA</td>
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</tr>
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<tr>
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<td></td>
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<td></td>
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<td>Plac-LuxR-Plux-NagZ</td>
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</tr>
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</table>

Revision as of 12:54, 20 August 2013

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Tranformation and cloning constructs

Sender cells Receiver cells
AHL expressing system Fluorescent reporters system Enzyme reaction-based reporter system
Pcons-LuxI 1 (Strong) Plac-LuxR-Plux-GFP Plac-LuxR-Plux-LacZ
Pcons-LuxI 2 Plac-LuxR-Plux-YFP Plac-LuxR-Plux-Aes
Pcons-LuxI 3 Plac-LuxR-Plux-CFP Plac-LuxR-Plux-GusA
Pcons-LuxI 4 (Weak) Plac-LuxR-Plux-PhoA
Plac-LuxR-Plux-NagZ