"
Team:NTU-Taida/Parts
From 2013.igem.org
(Difference between revisions)
(→Reporter) |
|||
| (12 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar|Title=Part}}{{:Team:NTU-Taida/Templates/ContentStart}} | {{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar|Title=Part}}{{:Team:NTU-Taida/Templates/ContentStart}} | ||
| + | |||
| + | ==Note== | ||
| + | Some plasmids are not submitted due to similar parts in registry or time limit while they are still important fragments in our projects and we still lists them below. | ||
| + | |||
| + | Also, BBa_K575024 and BBa_K575033 submitted from the Northwestern help a lot. | ||
==Original Biobrick== | ==Original Biobrick== | ||
<html> | <html> | ||
| - | <table> | + | <table class="tableizer-table"> |
<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | <tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | ||
| - | <tr><td>BBa_K1157000</td><td>Regulatory</td><td>promoter sequence for PQS quorum sensing</td><td>341</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157000"> BBa_K1157000</a></td><td>Regulatory</td><td>promoter sequence for PQS quorum sensing</td><td>341</td></tr> |
| - | <tr><td>BBa_K1157001</td><td>Coding</td><td>PQSR</td><td>999</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157001">BBa_K1157001</a></td><td>Coding</td><td>PQSR</td><td>999</td></tr> |
| - | <tr><td>BBa_K1157003</td><td>Coding</td><td>AbaR</td><td>597</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157003">BBa_K1157003</a></td><td>Coding</td><td>AbaR</td><td>597</td></tr> |
</table> | </table> | ||
</html> | </html> | ||
==Reporter== | ==Reporter== | ||
| - | <table> | + | <html> |
| + | <table class="tableizer-table"> | ||
<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | <tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | ||
| - | <tr><td>BBa_K1157005</td><td>Reporter</td><td>Luciferase generator</td><td>1094</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157005">BBa_K1157005</a></td><td>Reporter</td><td>Luciferase generator</td><td>1094</td></tr> |
<tr><td> </td><td>Reporter</td><td>RBS-mTagBFP-tt</td><td>849</td></tr> | <tr><td> </td><td>Reporter</td><td>RBS-mTagBFP-tt</td><td>849</td></tr> | ||
| - | |||
<tr><td> </td><td>Reporter</td><td>RBS-mCherry-tt</td><td>858</td></tr> | <tr><td> </td><td>Reporter</td><td>RBS-mCherry-tt</td><td>858</td></tr> | ||
| - | |||
<tr><td> </td><td>Reporter</td><td>RBS-GFP-tt</td><td>867</td></tr> | <tr><td> </td><td>Reporter</td><td>RBS-GFP-tt</td><td>867</td></tr> | ||
| - | |||
</table> | </table> | ||
</html> | </html> | ||
| Line 26: | Line 29: | ||
==Protein Generator== | ==Protein Generator== | ||
<html> | <html> | ||
| - | <table> | + | <table class="tableizer-table"> |
<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | <tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | ||
| - | <tr><td>BBa_K1157007</td><td>Coding</td><td>Quorum sensing receptor pqsR generator</td><td>1157</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157007">BBa_K1157007</a></td><td>Coding</td><td>Quorum sensing receptor pqsR generator</td><td>1157</td></tr> |
| - | <tr><td>BBa_K1157017</td><td>Composite</td><td>pPQS-GFPmut3</td><td>1227</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157017">BBa_K1157017</a></td><td>Composite</td><td>pPQS-GFPmut3</td><td>1227</td></tr> |
| - | <tr><td>BBa_K1157019</td><td>Composite</td><td>Pc-PQSR</td><td>1200</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157019">BBa_K1157019</a></td><td>Composite</td><td>Pc-PQSR</td><td>1200</td></tr> |
| - | <tr><td> </td><td> | + | <tr><td> </td><td>Composite</td><td>RBS-RhlR-tt</td><td>873</td></tr> |
| - | <tr><td> </td><td> | + | <tr><td> </td><td>Composite</td><td>RBS-LasR-tt</td><td>867</td></tr> |
| - | + | <tr><td> </td><td>Composite</td><td>RBS-CinR-tt</td><td>931</td></tr> | |
| - | <tr><td> </td><td> | + | <tr><td> </td><td>Composite</td><td>RBS-AbaR-tt</td><td>741</td></tr> |
| - | <tr><td> </td><td> | + | |
</table> | </table> | ||
</html> | </html> | ||
| Line 41: | Line 43: | ||
==Without positive feedback circuits== | ==Without positive feedback circuits== | ||
<html> | <html> | ||
| - | <table> | + | <table class="tableizer-table"> |
<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | <tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | ||
| - | <tr><td>BBa_K1157010</td><td>Composite</td><td>Pc-RhlR-mCherry</td><td>1871</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157010">BBa_K1157010</a></td><td>Composite</td><td>Pc-RhlR-mCherry</td><td>1871</td></tr> |
| - | <tr><td>BBa_K1157016</td><td>Composite</td><td>Pc-LasR-pLas-BFP</td><td>1960</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157016">BBa_K1157016</a></td><td>Composite</td><td>Pc-LasR-pLas-BFP</td><td>1960</td></tr> |
| - | <tr><td>BBa_K575033</td><td>Composite</td><td> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_KK575033">BBa_K575033</a></td><td>Composite</td><td>PRhl-GFP-Pc-RhlR</td><td>1600</td></tr> |
| - | <tr><td> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K575024">BBa_K575024</a></td><td>Composite</td><td>Plas-GFP-Pc-LasR</td><td>1701</td></tr> |
| - | <tr><td>BBa_K1157008</td><td>Composite</td><td>pLas-BFP-Pc-LasR</td><td>2018</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157008">BBa_K1157008</a></td><td>Composite</td><td>pLas-BFP-Pc-LasR</td><td>2018</td></tr> |
| - | <tr><td> </td><td> | + | <tr><td> </td><td>Composite</td><td>pPQS-GFP-Pc-PQSR</td><td>2401</td></tr> |
</table> | </table> | ||
</html> | </html> | ||
| Line 54: | Line 56: | ||
==Positive Feedback circuit== | ==Positive Feedback circuit== | ||
<html> | <html> | ||
| - | <table> | + | <table class="tableizer-table"> |
<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | <tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | ||
| - | <tr><td>BBa_K1157020</td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-GFPmut3</td><td>2635</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157020">BBa_K1157020</a></td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-GFPmut3</td><td>2635</td></tr> |
| - | <tr><td>BBa_K1157011</td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-BFP</td><td>2620</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157011">BBa_K1157011</a></td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-BFP</td><td>2620</td></tr> |
| - | <tr><td>BBa_K1157012</td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-luciferase</td><td>2851</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157012">BBa_K1157012</a></td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-luciferase</td><td>2851</td></tr> |
| - | <tr><td>BBa_K1157018</td><td>Composite</td><td>pRhl-RhlR-mCherry-Pc-RhlR</td><td>2629</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157018">BBa_K1157018</a></td><td>Composite</td><td>pRhl-RhlR-mCherry-Pc-RhlR</td><td>2629</td></tr> |
| - | <tr><td>BBa_K1157021</td><td>Composite</td><td>pRhl-RhlR-GFP-Pc-RhlR</td><td>2635</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157021">BBa_K1157021</a></td><td>Composite</td><td>pRhl-RhlR-GFP-Pc-RhlR</td><td>2635</td></tr> |
| - | <tr><td>BBa_K1157014</td><td>Composite</td><td>pLas-LasR-GFPmut3-Pc-LasR</td><td>2727</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157014">BBa_K1157014</a></td><td>Composite</td><td>pLas-LasR-GFPmut3-Pc-LasR</td><td>2727</td></tr> |
</table> | </table> | ||
</html> | </html> | ||
| Line 67: | Line 69: | ||
==Negative Regulation== | ==Negative Regulation== | ||
<html> | <html> | ||
| - | <table> | + | <table class="tableizer-table"> |
<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | <tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr> | ||
| - | <tr><td>BBa_K1157013</td><td>Composite</td><td>pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI</td><td>3627</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157013">BBa_K1157013</a></td><td>Composite</td><td>pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI</td><td>3627</td></tr> |
| - | <tr><td>BBa_K1157015</td><td>Composite</td><td>pConst-Rhl-pRhl-RhlR-CI-pCI-mCherry</td><td>3627</td></tr> | + | <tr><td><a href="http://parts.igem.org/Part:BBa_K1157015">BBa_K1157015</a></td><td>Composite</td><td>pConst-Rhl-pRhl-RhlR-CI-pCI-mCherry</td><td>3627</td></tr> |
</table> | </table> | ||
</html> | </html> | ||
{{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Part}} | {{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Part}} | ||
Latest revision as of 03:10, 28 September 2013
Part
Contents |
Note
Some plasmids are not submitted due to similar parts in registry or time limit while they are still important fragments in our projects and we still lists them below.
Also, BBa_K575024 and BBa_K575033 submitted from the Northwestern help a lot.
Original Biobrick
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157000 | Regulatory | promoter sequence for PQS quorum sensing | 341 |
| BBa_K1157001 | Coding | PQSR | 999 |
| BBa_K1157003 | Coding | AbaR | 597 |
Reporter
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157005 | Reporter | Luciferase generator | 1094 |
| Reporter | RBS-mTagBFP-tt | 849 | |
| Reporter | RBS-mCherry-tt | 858 | |
| Reporter | RBS-GFP-tt | 867 |
Protein Generator
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157007 | Coding | Quorum sensing receptor pqsR generator | 1157 |
| BBa_K1157017 | Composite | pPQS-GFPmut3 | 1227 |
| BBa_K1157019 | Composite | Pc-PQSR | 1200 |
| Composite | RBS-RhlR-tt | 873 | |
| Composite | RBS-LasR-tt | 867 | |
| Composite | RBS-CinR-tt | 931 | |
| Composite | RBS-AbaR-tt | 741 |
Without positive feedback circuits
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157010 | Composite | Pc-RhlR-mCherry | 1871 |
| BBa_K1157016 | Composite | Pc-LasR-pLas-BFP | 1960 |
| BBa_K575033 | Composite | PRhl-GFP-Pc-RhlR | 1600 |
| BBa_K575024 | Composite | Plas-GFP-Pc-LasR | 1701 |
| BBa_K1157008 | Composite | pLas-BFP-Pc-LasR | 2018 |
| Composite | pPQS-GFP-Pc-PQSR | 2401 |
Positive Feedback circuit
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157020 | Composite | Pc-RhlR-pRhl-RhlR-GFPmut3 | 2635 |
| BBa_K1157011 | Composite | Pc-RhlR-pRhl-RhlR-BFP | 2620 |
| BBa_K1157012 | Composite | Pc-RhlR-pRhl-RhlR-luciferase | 2851 |
| BBa_K1157018 | Composite | pRhl-RhlR-mCherry-Pc-RhlR | 2629 |
| BBa_K1157021 | Composite | pRhl-RhlR-GFP-Pc-RhlR | 2635 |
| BBa_K1157014 | Composite | pLas-LasR-GFPmut3-Pc-LasR | 2727 |
Negative Regulation
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157013 | Composite | pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI | 3627 |
| BBa_K1157015 | Composite | pConst-Rhl-pRhl-RhlR-CI-pCI-mCherry | 3627 |
