Team:Heidelberg/AttributionsII

From 2013.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 23: Line 23:
             <div>
             <div>
                     <div>
                     <div>
-
                                 <h1><span style="font-size:180%;color:#800000;">Attributions. </span><span class="text-muted" style="font-size:100%"> Thank You very much!</span></h1>       
+
                                 <h1><span style="font-size:180%;color:#7137c8;">Attributions. </span><span class="text-muted" style="font-size:100%"> Thank You very much!</span></h1>       
                     </div>
                     </div>
                 </div>
                 </div>
             <div>
             <div>
-
                 <h2 style="color:#800000">iGEM Team Heidelberg 2013 </h2>
+
                 <h2 style="color:#7137c8">iGEM Team Heidelberg 2013 </h2>
                 <h3 style=""text-align:middle; height:100px"> Ilia Kats </h3>
                 <h3 style=""text-align:middle; height:100px"> Ilia Kats </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
-
                  One of our team members, the secret nerd in chief of our group, Ilia Kats, had the initial idea for our project and inspired us all with his dream of easy and fast recycling of gold. He also came up with the concept to exploit the NRPS modularity for setting up the NRPS designer.<br />
+
                One of our team members, the secret nerd in chief of our group, Ilia Kats, had the initial idea for our project and inspired all of us with his dream of easy and efficient recycling of gold. He also came up with the concept to exploit the NRPS modularity for setting up the NRPSDesigner.<br />
-
                            Besides all of his brain work, Ilia was indispensable for all of the wetlab work for the different sub projects. He was single-handedly responsible for cloning the methylmalonyl-CoA patwhay for the Delftibactin project. In the Synthetic Peptides, Indigoidine-Tag and Tag-Optimization subproject he was involved in the pilot experiments. And last but not least, his programming skills were essential for the successful implementation of our NRPSDesigner software where he used his supernatural force to eliminate even the toughest bugs. <br />
+
                Besides all of his brainwork, Ilia was indispensable for all of the wetlab work for the different subprojects. He was single-handedly responsible for the cloning the methylmalonyl-CoA patwhay for the delftibactin project. In the synthetic peptides, the indigoidine-tag and tag-optimization subproject, he was involved in the pilot experiments. And last but not least, his programming skills were essential for the successfull implementation of our NRPSDesigner software where he used his supernatural force to eliminate even the toughest bugs. <br />
-
                            A big THANK U from the whole team for the inspiration and great humor.
+
                A big THANK U from the whole team for the inspiration and great humor.
                 </p>
                 </p>
                 </div>
                 </div>
Line 40: Line 40:
                 <h3 style="text-align:middle"> Synthetic Peptides</h3>
                 <h3 style="text-align:middle"> Synthetic Peptides</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
-
The main focus of this project was to demonstrate the feasibility of NRPS module shuffling. This was performed by Joshua Sachs, Philipp Walch, Tania Christiansen and Anja Riedel. They established the experimental strategy and designed all corresponding primers needed for the amplification of the different Tyrocidine modules. Tania Christiansen was especially responsible for the BioBrick submission and the cloning of the fragments into the needed RFCs.
+
              The main focus of this project was to demonstrate the feasibility of NRPS module shuffling, performed by Joshua Sachs, Philipp Walch, Tania Christiansen and Anja Riedel. They established the experimental strategy and designed all corresponding primers needed for the amplification of the different tyrocidine modules. Tania Christiansen was especially responsible for the BioBrick submission and the cloning of the fragments into the needed RFCs.
-
<br/>
+
                <br/>
-
Another essential part in the Synthetic Peptides project was the mass spectrometry of which Joshua Sachs was in charge. Everyone in this group contributed to the great success of this part of the project! Here, we want to thank our advisor Lorenz Adlung, who helped us with his great experience he shared in frequent lab-meeting discussions.
+
                Another essential part in the synthetic peptides subproject was the mass spectrometry, which Joshua Sachs was in charge of. Everyone in this group contributed to the great success of this part of the project! Here, we want to thank our advisor Lorenz Adlung, who helped us with his great experience he shared in frequent lab-meeting discussions.
                 </p>
                 </p>
                 </div>
                 </div>
Line 49: Line 49:
                 <h3 style="text-align:middle"> Indigoidine-Tag </h3>
                 <h3 style="text-align:middle"> Indigoidine-Tag </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
-
This subproject was about developing an easily detectable, inert and universal tag for NRPs. The work on the fusion peptides comprising modules from the Tyrocidine cluster were performed by Joshua Sachs, Philipp Walch, Tania Christiansen and Anja Riedel. Philipp Walch and Joshua Sachs performed the valine-indigoidine as well as the di- and tripeptide-indigoidine tagging, Anja Riedel took care of the linker variation. The ccdB-construct was generated by Joshua Sachs and Philipp Walch. Without the devotion of all of the people mentioned above, the project would not have been feasible.
+
              The indigoidine-tag subproject was about developing an easily detectable, inert and universal tag for NRPs. The work on the module-chains comprising fusion peptides from the tyrocidine cluster were performed by Joshua Sachs, Philipp Walch, Tania Christiansen and Anja Riedel. Philipp Walch and Joshua Sachs performed the valine-indigoidine as well as the di- and tripeptide-indigoidine tagging; Anja Riedel took care of the linker variation. Joshua Sachs and Philipp Walch generated the ccdB-construct. Without the devotion of all of the people mentioned above, the project would not have been feasible. Philipp Walch performed the biobricking of the submitted parts.
-
<br/>
+
              <br/>
-
We want to thank Dominik Niopek and Hanna Meyer for their expertise they shared with us when we were working on the RFC100.
+
                We want to thank Dominik Niopek and Hannah Meyer for their expertise when writing the RFC100.
                 </p>
                 </p>
                 </div>
                 </div>
Line 58: Line 58:
                 <h3 style="text-align:middle"> Tag Optimization </h3>
                 <h3 style="text-align:middle"> Tag Optimization </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
-
                 Ralf Beer, Konrad Herbst and Nikolaos Ignatiadis designed strategies for the optimization of the Indigoidine-Tag, which includes the creation of 58 engineered indigoidine synthetases and the development of a quantitative indigoidine assay. These hard-working fellows did not mind spending 48 hours in a row at the bench, cloning large plasmid libraries and performing hundreds of co-transformations, thereby establishing the RFC99. Konrad showed that one can write a script for almost any purpose. He contributed most to the evaluation of the indigoidine assay, the improvement of the Tag Optimization strategy and the RFC99. Nikos was involved in the design of the first constructs and was a great help in the creation of the synthetic T-domains. Ralf focussed on plasmid and primer design as well as the labwork and was actually considered to be integrated into the NRPSDesigner as an alternative sequence based domain prediction tool. But not only these three guys were infected by the blue fever - thanks to Hannah Meyer they always had an advisor at their side with whom they had many creative scientific and even more non-scientific but still creative discussions.
+
                 Ralf Beer, Konrad Herbst and Nikolaos Ignatiadis designed strategies for the optimization of the indigoidine-Tag, which includes the creation of 58 engineered indigoidine synthetases and the development of a quantitative time-resolved indigoidine assay. These hard-working fellows did not mind spending 48 hours in a row at the bench, generating and testing large plasmid libraries and performing hundreds of co-transformations, thereby establishing the RFC99. Meanwhile, Konrad proved that one can write a script for almost any purpose. He contributed most to the analysis of the indigoidine assay, the progress of the tag optimization strategy and the RFC99. Nikos was involved in the design of the first constructs and was a big help in the creation of the synthetic T-domains. Ralf focussed on plasmid and primer design as well as the labwork and was actually considered to be integrated into the NRPSDesigner as an alternative sequence-based domain prediction tool. But not only these three guys were infected by the blue fever - thanks to Hannah Meyer they always had an advisor at their side to engage in more and sometimes less scientific discussions.
                 </p>
                 </p>
                 </div>
                 </div>
Line 65: Line 65:
                 <h3 > Gold Recycling </h3>
                 <h3 > Gold Recycling </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
-
                 The cloning strategies and amplification of the Del cluster were performed and developed by Florian Schmidt, Sophie Rabe, Nils Kurzawa and Johanna Meichsner. The experiments started with the amplification of DelH executed by Johanna Meichsner which turned out to be one of the most challenging parts of our project. Johanna proved to be extremely resilient to recurrent setbacks and her power of endurance gave a great example to the whole team. Florian Schmidt, Sophie Rabe and Nils Kurzawa took care of the plasmid assembly of the remaining genes from the Del cluster. After DelRest was succesfully amplified, Florian Schmidt and Sophie Rabe worked as well on the assembly of the DelH plasmid and the gold precipitation by Delftibactin. Sophie Rabe was furthermore responsible of proteins assays, delftibactin purification and MICRO-TOF, Florian Schmidt entablished the protocol to dissolve electronic waste. All of them were extremely dedicated to their work and cloned day and night.<br />
+
                 The cloning strategies and amplification of the Del cluster were performed and developed by Florian Schmidt, Sophie Rabe, Nils Kurzawa and Johanna Meichsner. The experiments started with the amplification of DelH executed by Johanna Meichsner, which turned out to be one of the most challenging parts of our project. Johanna proved to be extremely resilient to recurrent setbacks and her power of endurance gave a great example to the whole team. Florian Schmidt, Sophie Rabe and Nils Kurzawa took care of the plasmid assembly of the remaining genes from the Del cluster. After DelRest was successfully amplified, Florian Schmidt and Sophie Rabe worked as well on the assembly of the DelH plasmid and the gold precipitation by delftibactin. Sophie Rabe was furthermore responsible of proteins assays, delftibactin purification and MICRO-TOF, Florian Schmidt established the protocol to dissolve electronic waste. All of them were extremely dedicated and cloned day and night.
-
                 Ilia Kats cloned the pIK8 plasmid and all prior plasmids necessary for the establishment of the methylmalonyl-CoA pathway. He performed all steps of the cloning procedure of these plasmids from primer design up to electroporation and screening. <br />
+
                <br />
-
                 Here we want to give a special thank you to our advisors Dominik Niopek, Fanny Georgi and Katharina Genreith for their great help in planning the cloning strategies and introducing us to the world of primer design! Thanks to Fanny for helping us with electroporations any time of the day (or night).
+
                 Ilia Kats cloned the pIK8 plasmid and all prior plasmids necessary for the establishment of the methylmalonyl-CoA pathway. He performed all steps of the cloning procedure of these plasmids from primer design up to electroporation and screening.  
 +
                <br />
 +
                 Here we want to give a special thank you to our advisors Dominik Niopek, Katharina Genreith and Fanny Georgi for their great help in planning of the cloning strategies and introducing us to the world of primer design! Thanks to Fanny for helping with electroporations any time of the day (or night).
                 </p>
                 </p>
                 </div>
                 </div>
Line 74: Line 76:
                 <h3 style="text-align:middle"> NRPSDesigner </h3>
                 <h3 style="text-align:middle"> NRPSDesigner </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
-
                 The software-core-team consisted of Konrad Herbst, Ilia Kats, Nikolaos Ignatiadis, Julia Schessner and Nils Kurzawa. They worked together on the implementation of the NRPSDesigner software and the establishment of the respective database. Additionally, Joshua Sachs, Anja Riedel and Philipp Walch contributed to the maintenance of the database. Furthermore, Ilia Kats was the administrator of our internal server. All of the people mentioned above worked on our sub-software project iGEM42. The advisors Hannah Meyer and Tim Heinemann helped, not only with the NRPSDesigner, but also with motivating the whole team. A big thanks to Hannah for baking all those cookies and to Tim for helping to eat them!
+
                 The software-core-team consisted of Konrad Herbst, Ilia Kats, Nikolaos Ignatiadis, Julia Schessner and Nils Kurzawa. They worked together on the implementation of the NRPSDesigner software and the establishment of the respective database. Additionally, Joshua Sachs, Anja Riedel, Julia Schessner and Philipp Walch contributed to the maintenance of the database. Furthermore, administrator Ilia Kats was in charge of our internal wiki and server. All of the software geeks mentioned above worked on our sub-software project iGEM42. The advisors Hannah Meyer and Tim Heinemann helped not only with the NRPSDesigner, but also with motivating the entire team. A big thanks to Hannah for bringing all these yummy cookies and to Tim for helping to eat them!
                 </p>
                 </p>
                 </div>
                 </div>
<div >
<div >
-
                 <h3 style="text-align:middle"> Wiki Design  </h3>
+
                 <h3 > Modeling </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
-
                 Our wiki is uses stylesheets from Bootstrap 3.0 and was implemented by Nils Kurzawa. He wrote the main javascripts and css style sheets used on this wiki. Nils created the main page together with the project pages and all templates. Tania Christiansen was responsible for all the pictures used for the wiki navigation. All team members worked together to generate the interface graphics and basic figures in inkskape. Especially Tania Christiansen and Anja Riedel could bring in their talent. Thanks also to our creative advisors Fanny Georgi and Katharina Genreit who came up with many ideas. After the basic implementation, Nils received help in the wiki design from Nikolaos Ignatiadis, Ilia Kats, Konrad Herbst and Johanna Meichsner. Important to mention is again Tim Heinemann here for his niggling but always helpful comments.
+
                 Modeling of the recombinant delftibactin production and the feasibility of the industrial scale gold recycling from electronic waste using delftibactin was mainly performed by Nikolaos Ignatiadis. On his journey through metabolic modeling of delftibactin producing <i>E. coli</i>, bioreactor simulations and cost estimations, he was supported by Johanna Meichsner and Joshua Sachs, who did research on financial data and Florian Schmidt on chemical equations. They received helpful advice by Tim Heinemann.
 +
                <br/>
 +
                The modeling of the production kinetics of indigoidine to disentangle the underlying mechanisms of bacterial growth and peptide synthesis was realized by the task force consisting of Konrad Herbst, Ilia Kats, supported by Lorenz Adlung. Konrad was responsible of processing of the time-resolved experimental data, which were generated by him and Ralf Beer. Ilia then generated a model to elucidate the influence of indigoidine production on bacterial growth of bacteria and to provide a more quantitative understanding of the synthesis efficiency of the different T domains and PPTases.
                 </p>
                 </p>
                 </div>
                 </div>
 +
<div >
 +
                <h3 style="text-align:middle"> Wiki Design  </h3>
 +
                <p style="text-align:justify">
 +
                Our wiki is mainly build on stylesheets from Bootstrap 3.0 and was implemented by Nils Kurzawa. He wrote the main javascripts and css stylesheets used on this wiki. Nils and Tim Heinemann came up with the idea of the main page and subsequently designed the templates for all other pages. Tania Christiansen was responsible several pictures such as the symbols used in the wiki navigation. All team members developed quite some talent when generating graphics and figures in Inkskape (yes Ilia, it is a great piece of software). Thanks also to our creative advisors Fanny Georgi (who should think of an alternative career as photographer) and Katharina Genreit who came up with many ideas. After the basic implementation was finished, Nils received help in the wiki design from Nikolaos Ignatiadis, Ilia Kats, Konrad Herbst and Johanna Meichsner. Important to mention is Tim Heinemann for his niggling but always helpful comments.
 +
                </p>
 +
                </div>
 +
<div>
 +
                <h3 style="text-align:middle"> Videos</h3>
 +
                <p style="text-align:justify">
 +
              We produced two videos to spread our project to a wider audience.
 +
<h4 style="text-align:middle"> Introduction to NRPS Whiteboard Animation </h4>
 +
This short clip explains the basics on NRPS and gives a very short outline of our project.<br/>
 +
Tania Christiansen is responsible for the script, illustration and audio/video production. Ralf Beer helped with the audio recording and processing. </p>
 +
<h4 style="text-align: middle"> RFC 99 & 100: Peptide Production Pipeline Video </h4>
 +
<p>
 +
This video demonstrates the implementation of our two RFCs: We show short sequences of the process and explain the single steps to encourage the community to use this new peptide production approach. <br/>
 +
Hanna Meichsner is responsible for the script and audio/video production. Ralph Beer, Tania Christiansen, Nikolaos Ignatidis, Sophie Rabe and Philipp Walch were recorded for several different scenes.
 +
                </p>
 +
                </div>
<div >
<div >
                 <h3 style="text-align:middle"> Human Practice</h3>
                 <h3 style="text-align:middle"> Human Practice</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
-
                 The entire team worked on our human practice projects. Philipp Walch is a member of the education initiative "Life-Science Lab" and organized the essay competition for the high school students. Philipp Walch, Sophie Rabe and Tania Christiansen presented our project at various events to the interested public together with members of the high school iGEm Team from 2012. Joshua Sachs who volunteers as a fire fighter established the contact with the army which gave us insight in the bioweapons defense program (ABC). Ralf Beer and Konrad Herbst received valuable input from Peptide Specialties Laboratories GmbH. Philipp Walch and Nikos Ignatiadis visited TBM Edelmetall Recycling. Johanna Meichsner introduced us to Dorothea van Aaken, who organizes environmental workshops for primary students. Fanny Georgi organized the contact to Dr. Zawatzky from German Cancer Research Center (DKFZ). Dominik Niopek introduced us to the science author Olaf Fritsche and Lorenz Adlung, who also writes, got us in contact with Isabel Marleen Pötzsch. Both Dominik Niopek and Lorenz Adlung also helped with the "not invented by nature" collaboration.
+
                 The entire team worked on our human practice projects. Philipp Walch is a member of the education initiative "Life-Science Lab" and organized the essay competition for the high school students. Philipp Walch, Sophie Rabe and Tania Christiansen presented our project at various events to the interested public together with members of the high school iGEM Team from 2012. Joshua Sachs who volunteers as a fire fighter established the contact with the army, which gave us insight in the bio-weapons defense program (ABC). Ralf Beer and Konrad Herbst received valuable input from Peptide Specialties Laboratories GmbH. Philipp Walch and Nikos Ignatiadis visited TBM Edelmetall Recycling. Johanna Meichsner introduced us to Dorothea van Aaken, who organizes environmental workshops for primary students. Fanny Georgi organized the contact to Dr. Zawatzky from German Cancer Research Center (DKFZ). Dominik Niopek introduced us to the science author Olaf Fritsche and Lorenz Adlung, who also writes, got us in contact with Isabel Marleen Pötzsch. Both Dominik Niopek and Lorenz Adlung also helped with the "not invented by nature" collaboration.
<br/>
<br/>
<br/>
<br/>
Line 96: Line 119:
             <div>
             <div>
-
                 <h2 style="color:#800000">BioQuant, University of Heidelberg </h2>
+
                 <h2 style="color:#7137c8">BioQuant, University of Heidelberg </h2>
                 <h3 style=""text-align:middle"> Prof. Dr. Thomas Höfer </h3>
                 <h3 style=""text-align:middle"> Prof. Dr. Thomas Höfer </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 121: Line 144:
             <div>
             <div>
-
                 <h2 style="color:#800000">Center for Molecular Biology Heidelberg (ZMBH), University of Heidelberg</h2>
+
                 <h2 style="color:#7137c8">Center for Molecular Biology Heidelberg (ZMBH), University of Heidelberg</h2>
                 <h3 style=""text-align:middle"> Core Facility for Mass Spectronomy and Proteomics </h3>
                 <h3 style=""text-align:middle"> Core Facility for Mass Spectronomy and Proteomics </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 136: Line 159:
             <div>
             <div>
-
                 <h2 style="color:#800000"> Department of Biochemistry, Philipps-University Marburg</h2>
+
                 <h2 style="color:#7137c8"> Department of Biochemistry, Philipps-University Marburg</h2>
                 <h3 style=""text-align:middle"> Prof. Dr. Mohamed Marahiel</h3>
                 <h3 style=""text-align:middle"> Prof. Dr. Mohamed Marahiel</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 146: Line 169:
             <div>
             <div>
-
                 <h2 style="color:#800000"> Department of Biosystems Science and Engineering, ETH Zurich</h2>
+
                 <h2 style="color:#7137c8"> Department of Biosystems Science and Engineering, ETH Zurich</h2>
                 <h3 style=""text-align:middle"> Prof. Dr. Martin Fussenegger</h3>
                 <h3 style=""text-align:middle"> Prof. Dr. Martin Fussenegger</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 156: Line 179:
             <div>
             <div>
-
                 <h2 style="color:#800000">Department of Chemical and Biological Engineering, University of Buffalo</h2>
+
                 <h2 style="color:#7137c8">Department of Chemical and Biological Engineering, University of Buffalo</h2>
                 <h3 style=""text-align:middle"> Prof. Blaine Pfeifer</h3>
                 <h3 style=""text-align:middle"> Prof. Blaine Pfeifer</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 166: Line 189:
             <div>
             <div>
-
                 <h2 style="color:#800000"> ESRC Centre for Economic and Social Aspects of Genomics (Cesagen), Lancaster University, Institute of Advanced Studies (UNU-IAS), United Nations University</h2>
+
                 <h2 style="color:#7137c8"> ESRC Centre for Economic and Social Aspects of Genomics (Cesagen), Lancaster University, Institute of Advanced Studies (UNU-IAS), United Nations University</h2>
                 <h3 style=""text-align:middle">Dr. Paul Oldham</h3>
                 <h3 style=""text-align:middle">Dr. Paul Oldham</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 176: Line 199:
             <div>
             <div>
-
                 <h2 style="color:#800000">German Cancer Research Center (DKFZ), Heidelberg</h2>
+
                 <h2 style="color:#7137c8">German Cancer Research Center (DKFZ), Heidelberg</h2>
                 <h3 style=""text-align:middle"> Prof. Dr. Stephan Herzig </h3>
                 <h3 style=""text-align:middle"> Prof. Dr. Stephan Herzig </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 196: Line 219:
             <div>
             <div>
-
                 <h2 style="color:#800000">Harvard Wyss Institute for Biologically Inspired Engineering, Center for Life Sciences, Boston</h2>
+
                 <h2 style="color:#7137c8">Harvard Wyss Institute for Biologically Inspired Engineering, Center for Life Sciences, Boston</h2>
                 <h3 style=""text-align:middle"> Dr. Matthew Mattozzi</h3>
                 <h3 style=""text-align:middle"> Dr. Matthew Mattozzi</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 206: Line 229:
             <div>
             <div>
-
                 <h2 style="color:#800000">Heidelberg Technology Park UniTT Start-Up Centre, University of Heidelberg</h2>
+
                 <h2 style="color:#7137c8">Heidelberg Technology Park UniTT Start-Up Centre, University of Heidelberg</h2>
                 <h3 style=""text-align:middle"> Dr. Raoul Haschke</h3>
                 <h3 style=""text-align:middle"> Dr. Raoul Haschke</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 216: Line 239:
             <div>
             <div>
-
                 <h2 style="color:#800000">Institute of Chemical Engineering, University of Vienna</h2>
+
                 <h2 style="color:#7137c8">Institute of Chemical Engineering, University of Vienna</h2>
                 <h3 style=""text-align:middle"> Dipl.-Ing.Paul Kroll</h3>
                 <h3 style=""text-align:middle"> Dipl.-Ing.Paul Kroll</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 226: Line 249:
             <div>
             <div>
-
                 <h2 style="color:#800000">Institute of Organic Chemistry, University of Heidelberg</h2>
+
                 <h2 style="color:#7137c8">Institute of Organic Chemistry, University of Heidelberg</h2>
                 <h3 style=""text-align:middle"> Mass Spectrometry Facility</h3>
                 <h3 style=""text-align:middle"> Mass Spectrometry Facility</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 236: Line 259:
             <div>
             <div>
-
                 <h2 style="color:#800000">Institute of Pharmacology and Molecular Biotechnology (IPMB), University of Heidelberg</h2>
+
                 <h2 style="color:#7137c8">Institute of Pharmacology and Molecular Biotechnology (IPMB), University of Heidelberg</h2>
                 <h3 style=""text-align:middle"> Prof. Dr. Christian Klein and Lena Weigel </h3>
                 <h3 style=""text-align:middle"> Prof. Dr. Christian Klein and Lena Weigel </h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 256: Line 279:
             <div>
             <div>
-
                 <h2 style="color:#800000">Peptide Specialties Laboratories GmbH, Heidelberg</h2>
+
                 <h2 style="color:#7137c8">Peptide Specialties Laboratories GmbH, Heidelberg</h2>
                 <h3 style=""text-align:middle"> Dr. Hans-Richard Rackwitz</h3>
                 <h3 style=""text-align:middle"> Dr. Hans-Richard Rackwitz</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 266: Line 289:
           <div>
           <div>
-
                 <h2 style="color:#800000">School of Medicine, University of Maryland</h2>
+
                 <h2 style="color:#7137c8">School of Medicine, University of Maryland</h2>
                 <h3 style=""text-align:middle"> Prof. Jacques Ravel, Ph.D.</h3>
                 <h3 style=""text-align:middle"> Prof. Jacques Ravel, Ph.D.</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">
Line 274: Line 297:
                 </p>
                 </p>
           </div>
           </div>
-
 
+
        <div>
 +
                <h2 style="color:#7137c8">The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark</h2>
 +
                <h3 style=""text-align:middle"> Dr. Kai Zhuang </h3>
 +
                <p style="text-align:justify">
 +
                Dr. Kai Zhuang gave us very helpful advice on integrating constraint based models of metabolism with bioreactor process descriptions.
 +
<br/>
 +
<br/>
 +
                </p>
 +
          </div>
             <div>
             <div>
-
                 <h2 style="color:#800000">University Medical Center Heidelberg, University of Heidelberg</h2>
+
                 <h2 style="color:#7137c8">University Medical Center Heidelberg, University of Heidelberg</h2>
                 <h3 style=""text-align:middle"> Neonate Screening Facility</h3>
                 <h3 style=""text-align:middle"> Neonate Screening Facility</h3>
                 <p style="text-align:justify">
                 <p style="text-align:justify">

Latest revision as of 22:54, 28 October 2013


Attributions. Thank You very much!

iGEM Team Heidelberg 2013

Ilia Kats

One of our team members, the secret nerd in chief of our group, Ilia Kats, had the initial idea for our project and inspired all of us with his dream of easy and efficient recycling of gold. He also came up with the concept to exploit the NRPS modularity for setting up the NRPSDesigner.
Besides all of his brainwork, Ilia was indispensable for all of the wetlab work for the different subprojects. He was single-handedly responsible for the cloning the methylmalonyl-CoA patwhay for the delftibactin project. In the synthetic peptides, the indigoidine-tag and tag-optimization subproject, he was involved in the pilot experiments. And last but not least, his programming skills were essential for the successfull implementation of our NRPSDesigner software where he used his supernatural force to eliminate even the toughest bugs.
A big THANK U from the whole team for the inspiration and great humor.

Synthetic Peptides

The main focus of this project was to demonstrate the feasibility of NRPS module shuffling, performed by Joshua Sachs, Philipp Walch, Tania Christiansen and Anja Riedel. They established the experimental strategy and designed all corresponding primers needed for the amplification of the different tyrocidine modules. Tania Christiansen was especially responsible for the BioBrick submission and the cloning of the fragments into the needed RFCs.
Another essential part in the synthetic peptides subproject was the mass spectrometry, which Joshua Sachs was in charge of. Everyone in this group contributed to the great success of this part of the project! Here, we want to thank our advisor Lorenz Adlung, who helped us with his great experience he shared in frequent lab-meeting discussions.

Indigoidine-Tag

The indigoidine-tag subproject was about developing an easily detectable, inert and universal tag for NRPs. The work on the module-chains comprising fusion peptides from the tyrocidine cluster were performed by Joshua Sachs, Philipp Walch, Tania Christiansen and Anja Riedel. Philipp Walch and Joshua Sachs performed the valine-indigoidine as well as the di- and tripeptide-indigoidine tagging; Anja Riedel took care of the linker variation. Joshua Sachs and Philipp Walch generated the ccdB-construct. Without the devotion of all of the people mentioned above, the project would not have been feasible. Philipp Walch performed the biobricking of the submitted parts.
We want to thank Dominik Niopek and Hannah Meyer for their expertise when writing the RFC100.

Tag Optimization

Ralf Beer, Konrad Herbst and Nikolaos Ignatiadis designed strategies for the optimization of the indigoidine-Tag, which includes the creation of 58 engineered indigoidine synthetases and the development of a quantitative time-resolved indigoidine assay. These hard-working fellows did not mind spending 48 hours in a row at the bench, generating and testing large plasmid libraries and performing hundreds of co-transformations, thereby establishing the RFC99. Meanwhile, Konrad proved that one can write a script for almost any purpose. He contributed most to the analysis of the indigoidine assay, the progress of the tag optimization strategy and the RFC99. Nikos was involved in the design of the first constructs and was a big help in the creation of the synthetic T-domains. Ralf focussed on plasmid and primer design as well as the labwork and was actually considered to be integrated into the NRPSDesigner as an alternative sequence-based domain prediction tool. But not only these three guys were infected by the blue fever - thanks to Hannah Meyer they always had an advisor at their side to engage in more and sometimes less scientific discussions.

Gold Recycling

The cloning strategies and amplification of the Del cluster were performed and developed by Florian Schmidt, Sophie Rabe, Nils Kurzawa and Johanna Meichsner. The experiments started with the amplification of DelH executed by Johanna Meichsner, which turned out to be one of the most challenging parts of our project. Johanna proved to be extremely resilient to recurrent setbacks and her power of endurance gave a great example to the whole team. Florian Schmidt, Sophie Rabe and Nils Kurzawa took care of the plasmid assembly of the remaining genes from the Del cluster. After DelRest was successfully amplified, Florian Schmidt and Sophie Rabe worked as well on the assembly of the DelH plasmid and the gold precipitation by delftibactin. Sophie Rabe was furthermore responsible of proteins assays, delftibactin purification and MICRO-TOF, Florian Schmidt established the protocol to dissolve electronic waste. All of them were extremely dedicated and cloned day and night.
Ilia Kats cloned the pIK8 plasmid and all prior plasmids necessary for the establishment of the methylmalonyl-CoA pathway. He performed all steps of the cloning procedure of these plasmids from primer design up to electroporation and screening.
Here we want to give a special thank you to our advisors Dominik Niopek, Katharina Genreith and Fanny Georgi for their great help in planning of the cloning strategies and introducing us to the world of primer design! Thanks to Fanny for helping with electroporations any time of the day (or night).

NRPSDesigner

The software-core-team consisted of Konrad Herbst, Ilia Kats, Nikolaos Ignatiadis, Julia Schessner and Nils Kurzawa. They worked together on the implementation of the NRPSDesigner software and the establishment of the respective database. Additionally, Joshua Sachs, Anja Riedel, Julia Schessner and Philipp Walch contributed to the maintenance of the database. Furthermore, administrator Ilia Kats was in charge of our internal wiki and server. All of the software geeks mentioned above worked on our sub-software project iGEM42. The advisors Hannah Meyer and Tim Heinemann helped not only with the NRPSDesigner, but also with motivating the entire team. A big thanks to Hannah for bringing all these yummy cookies and to Tim for helping to eat them!

Modeling

Modeling of the recombinant delftibactin production and the feasibility of the industrial scale gold recycling from electronic waste using delftibactin was mainly performed by Nikolaos Ignatiadis. On his journey through metabolic modeling of delftibactin producing E. coli, bioreactor simulations and cost estimations, he was supported by Johanna Meichsner and Joshua Sachs, who did research on financial data and Florian Schmidt on chemical equations. They received helpful advice by Tim Heinemann.
The modeling of the production kinetics of indigoidine to disentangle the underlying mechanisms of bacterial growth and peptide synthesis was realized by the task force consisting of Konrad Herbst, Ilia Kats, supported by Lorenz Adlung. Konrad was responsible of processing of the time-resolved experimental data, which were generated by him and Ralf Beer. Ilia then generated a model to elucidate the influence of indigoidine production on bacterial growth of bacteria and to provide a more quantitative understanding of the synthesis efficiency of the different T domains and PPTases.

Wiki Design

Our wiki is mainly build on stylesheets from Bootstrap 3.0 and was implemented by Nils Kurzawa. He wrote the main javascripts and css stylesheets used on this wiki. Nils and Tim Heinemann came up with the idea of the main page and subsequently designed the templates for all other pages. Tania Christiansen was responsible several pictures such as the symbols used in the wiki navigation. All team members developed quite some talent when generating graphics and figures in Inkskape (yes Ilia, it is a great piece of software). Thanks also to our creative advisors Fanny Georgi (who should think of an alternative career as photographer) and Katharina Genreit who came up with many ideas. After the basic implementation was finished, Nils received help in the wiki design from Nikolaos Ignatiadis, Ilia Kats, Konrad Herbst and Johanna Meichsner. Important to mention is Tim Heinemann for his niggling but always helpful comments.

Videos

We produced two videos to spread our project to a wider audience.

Introduction to NRPS Whiteboard Animation

This short clip explains the basics on NRPS and gives a very short outline of our project.
Tania Christiansen is responsible for the script, illustration and audio/video production. Ralf Beer helped with the audio recording and processing.

RFC 99 & 100: Peptide Production Pipeline Video

This video demonstrates the implementation of our two RFCs: We show short sequences of the process and explain the single steps to encourage the community to use this new peptide production approach.
Hanna Meichsner is responsible for the script and audio/video production. Ralph Beer, Tania Christiansen, Nikolaos Ignatidis, Sophie Rabe and Philipp Walch were recorded for several different scenes.

Human Practice

The entire team worked on our human practice projects. Philipp Walch is a member of the education initiative "Life-Science Lab" and organized the essay competition for the high school students. Philipp Walch, Sophie Rabe and Tania Christiansen presented our project at various events to the interested public together with members of the high school iGEM Team from 2012. Joshua Sachs who volunteers as a fire fighter established the contact with the army, which gave us insight in the bio-weapons defense program (ABC). Ralf Beer and Konrad Herbst received valuable input from Peptide Specialties Laboratories GmbH. Philipp Walch and Nikos Ignatiadis visited TBM Edelmetall Recycling. Johanna Meichsner introduced us to Dorothea van Aaken, who organizes environmental workshops for primary students. Fanny Georgi organized the contact to Dr. Zawatzky from German Cancer Research Center (DKFZ). Dominik Niopek introduced us to the science author Olaf Fritsche and Lorenz Adlung, who also writes, got us in contact with Isabel Marleen Pötzsch. Both Dominik Niopek and Lorenz Adlung also helped with the "not invented by nature" collaboration.

BioQuant, University of Heidelberg

Prof. Dr. Thomas Höfer

Prof. Dr. Thomas Höfer's group provided us a sample of E. coli BL21 (DE3) (pLys).

Thi Bach Nga Ly-Hartig

Nga Ly-Hartig got us in touch with several research groups working with mass spectrometry and supported us with great cupcakes.

Prof. Dr. Robert B. Russel

Prof. Russel supplied us with a vial of E. coli BL21 (DE3).

Dr. Oliver Wichmann

Dr. Wichmann supported us with his equipment especially by allowing us to use his lyophilizer.

Center for Molecular Biology Heidelberg (ZMBH), University of Heidelberg

Core Facility for Mass Spectronomy and Proteomics

Dr. Thomas Ruppert and Dr. Bernd Hessling helped us with Mass Spectrometry measurements for the detection of our non-ribosomal peptides.

Dr. Oliver Gruss

The Gruss Lab kindly provided us the E. coli BL21 (DE3) (pLys) strain.

Department of Biochemistry, Philipps-University Marburg

Prof. Dr. Mohamed Marahiel

Prof. Dr. Marahiel kindly provided us with the Brevibacillus parabrevis ATCC 8185 strain and contributed helpful feedback on NRPS and our NRPSDesigner.

Department of Biosystems Science and Engineering, ETH Zurich

Prof. Dr. Martin Fussenegger

Prof. Fussenegger send us the expression plasmids for bpsA and svp, codon optimized for mammalian cells.

Department of Chemical and Biological Engineering, University of Buffalo

Prof. Blaine Pfeifer

Lei Fang from the Pfeifer Lab kindly provided us with the E. coli BAP1 strain and the pLF03 and pKD46 plasmids.

ESRC Centre for Economic and Social Aspects of Genomics (Cesagen), Lancaster University, Institute of Advanced Studies (UNU-IAS), United Nations University

Dr. Paul Oldham

For kindly supporting us with his expertise in information processing, interpretation of the social aspects of synthetic biology and for sharing his collection of synthetic biology terms with us.

German Cancer Research Center (DKFZ), Heidelberg

Prof. Dr. Stephan Herzig

Prof. Herzig and his group kindly offered the possibility to use their electroporator.

Dr. Dirk Nettelbeck

Dr. Nettelbeck, Ph.D.contributed with valuable input on homologous recombination.

Prof. Dr. Rainer Zawatzky

Prof. Zawatzky gave us lots of feedback on safety considerations and ethical concerns.

Harvard Wyss Institute for Biologically Inspired Engineering, Center for Life Sciences, Boston

Dr. Matthew Mattozzi

Dr. Mattozzi supported us with valuable advice on genomic homologous recombination. Dr. Mattozzi also kindly provided the pET21c-pcc-acc-mmce plasmid. His PhD student Marika Ziesack, M.sc. kindly introduced us to Gibson Cloning.

Heidelberg Technology Park UniTT Start-Up Centre, University of Heidelberg

Dr. Raoul Haschke

Dr. Haschke opened up new economical perspectives on the possible industrial scale NRP production and theidea to start our own company.

Institute of Chemical Engineering, University of Vienna

Dipl.-Ing.Paul Kroll

Mr. Kroll contributed with great scientific input on modelling the industrial scale Delftibactin production in E. coli.

Institute of Organic Chemistry, University of Heidelberg

Mass Spectrometry Facility

Prof. Groß kindly provided us with the possibility to use his HR-ESI MS and helped with data analysis.

Institute of Pharmacology and Molecular Biotechnology (IPMB), University of Heidelberg

Prof. Dr. Christian Klein and Lena Weigel

Prof. Klein kindly allowed us to use the MICRO-TOF of his group. Special thanks to Lena Weigel who helped us with the operation.

Dr. Holger Schäfer

Dr. Schäfer supported us with scientific and moral support during the cloning of the Del constructs.

Dr. Richard Wombacher

Marcel Best from Dr. Wombacher's group saved us with the last minute supply of a rotary evaporator!

Peptide Specialties Laboratories GmbH, Heidelberg

Dr. Hans-Richard Rackwitz

Dr. Rackwitz gave us fascinating insights in chemical peptide synthesis and valuable scientific input on our project.

School of Medicine, University of Maryland

Prof. Jacques Ravel, Ph.D.

Professor Jacques Ravel continuously helped us with the automated domain prediction pipeline of the NRPSDesigner software, for example by providing us with seed alignments for the Hidden Markov Models of his own PKS/NRPS analysis tool . He also tested the NRPSDesigner software and gave us valuable feedback.

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark

Dr. Kai Zhuang

Dr. Kai Zhuang gave us very helpful advice on integrating constraint based models of metabolism with bioreactor process descriptions.

University Medical Center Heidelberg, University of Heidelberg

Neonate Screening Facility

Dr. Dirk Kohlmüller and Dr. Kathrin Schmidt supported us in amino acid level detection by mass spectrometry.

Thanks to

Retrieved from "http://2013.igem.org/Team:Heidelberg/AttributionsII"