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Team:ETH Zurich/Experiments
From 2013.igem.org
(Difference between revisions)
| Line 71: | Line 71: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pLuxR</td> |
| - | <td> | + | <td>R0062</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Mutagenized | + | <td>Mutagenized PLuxR_1</td> |
<td>Optimized Biobrick !</td> | <td>Optimized Biobrick !</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Mutagenized | + | <td>Mutagenized PLuxR_2</td> |
<td>Optimized Biobrick !</td> | <td>Optimized Biobrick !</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>Mutagenized PLuxR_3</td> |
| - | <td> | + | <td>Optimized Biobrick !</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<h1><b>Final constructs</b></h1> | <h1><b>Final constructs</b></h1> | ||
| - | [[File:Constructs.png|1000px]] | + | [[File:Constructs.png|1000px|Thumb|<b>Figure 1. Transformed plasmids for mine cells and non-mine cells </b>]] |
<br clear="all"/> | <br clear="all"/> | ||
Revision as of 08:27, 3 September 2013
Tranformation and cloning constructs
| Sender cells | Receiver cells | |
|---|---|---|
| AHL expressing system | Fluorescent reporter system | Enzyme reaction-based reporter system |
| Pcons-LuxI 1 (Strong) | Plac-LuxR-Plux-GFP | Plac-LuxR-Plux-LacZ |
| Pcons-LuxI 2 | Plac-LuxR-Plux-YFP | Plac-LuxR-Plux-Aes |
| Pcons-LuxI 3 | Plac-LuxR-Plux-CFP | Plac-LuxR-Plux-GusA |
| Pcons-LuxI 4 (Weak) | Plac-LuxR-Plux-PhoA | |
| Plac-LuxR-Plux-NagZ | ||
| Biobrick | Registry part |
|---|---|
| LuxI | K805016 |
| LuxR | C0062 |
| Pcons 1 (Strong) | J23100 |
| Pcons 2 | J23118 |
| Pcons 3 | J23110 |
| Pcons 4 (Weak) | J23114 |
| pLuxR | R0062 |
| Mutagenized PLuxR_1 | Optimized Biobrick ! |
| Mutagenized PLuxR_2 | Optimized Biobrick ! |
| Mutagenized PLuxR_3 | Optimized Biobrick ! |
| Plac | I14032 |
| GFP | E0840 |
| YFP | E0030 |
| CFP | E0020 |
| LacZ | I732006 | GusA | New Biobrick ! |
| PhoA | New Biobrick ! |
| Aes | New Biobrick ! |
| NagZ | New Biobrick ! |
Final constructs
