Team:Heidelberg/Parts

From 2013.igem.org

(Difference between revisions)
Line 24: Line 24:
             <div class="row" style="float:left">
             <div class="row" style="float:left">
                 <div id="ProjectText" class="col-sm-12">
                 <div id="ProjectText" class="col-sm-12">
-
                  <h2 id="TeamParts">Team Parts</h2>
+
 
-
Our favorite part is <a href="http://parts.igem.org/Part:BBa_K1152007">BBa_K1152007</a>: Helper construct for NRP-Indigoidine-tagging.
+
<h2 id="Natural Parts">Natural Parts</h2>
-
<li> Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
+
-
<li> Compatibility with custom non-ribosomal peptide (NRP) synthesis.
+
-
<li> Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
+
-
<br><br>
+
We propose <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a> as Best new BioBrick Part, Natural, because:
We propose <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a> as Best new BioBrick Part, Natural, because:
Line 37: Line 33:
<br><br>
<br><br>
-
We propose <a href="http://parts.igem.org/Part:BBa_K1152007">BBa_K1152007</a> as Best new BioBrick Part or Device, Engineered, as mentioned above.
 
-
<br><br>
+
<h2 id="Engineered Parts">Engineered Parts</h2>
 +
 
We propose <a href="http://parts.igem.org/Part:BBa_K1152009">BBa_K1152009</a> as Most Improved Registry Part, because:
We propose <a href="http://parts.igem.org/Part:BBa_K1152009">BBa_K1152009</a> as Most Improved Registry Part, because:
<li> it encodes for sfp, a 4'-Phosphopanthetheinyl-transferase (PPTase) crucial for the activation of T domains
<li> it encodes for sfp, a 4'-Phosphopanthetheinyl-transferase (PPTase) crucial for the activation of T domains
<li> it improved the parts <a href="http://parts.igem.org/Part:BBa_K802006">BBa_K802006</a> and <a href="http://parts.igem.org/Part:BBa_K302010">BBa_K302010</a>, which do also encode for sfp from <i>Bacillus subtilis</i> but were deviating in three amino acids from the sequence published on <a href="http://www.ncbi.nlm.nih.gov/">NCBI</a>
<li> it improved the parts <a href="http://parts.igem.org/Part:BBa_K802006">BBa_K802006</a> and <a href="http://parts.igem.org/Part:BBa_K302010">BBa_K302010</a>, which do also encode for sfp from <i>Bacillus subtilis</i> but were deviating in three amino acids from the sequence published on <a href="http://www.ncbi.nlm.nih.gov/">NCBI</a>
<li> it is the first BioBrick in the registry that is explicitly encoding sfp, and it is annotated and characterized  
<li> it is the first BioBrick in the registry that is explicitly encoding sfp, and it is annotated and characterized  
 +
<br><br>
<br><br>
Line 50: Line 47:
<li>they were designed semi-rationally based on multiple sequence alignments
<li>they were designed semi-rationally based on multiple sequence alignments
<li>they exhibit distinct synthesis rates for Indigoidine depending on the T domains of this part collection
<li>they exhibit distinct synthesis rates for Indigoidine depending on the T domains of this part collection
 +
 +
<br><br>
 +
 +
<h2 id="Engineered Devices">Engineered Devices</h2>
 +
 +
Our favorite part is <a href="http://parts.igem.org/Part:BBa_K1152007">BBa_K1152007</a>: Helper construct for NRP-Indigoidine-tagging.
 +
<li> Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
 +
<li> Compatibility with custom non-ribosomal peptide (NRP) synthesis.
 +
<li> Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
 +
We propose <a href="http://parts.igem.org/Part:BBa_K1152007">BBa_K1152007</a> as Best new BioBrick Part or Device, Engineered, as mentioned above.
 +
<br><br>
<br><br>

Revision as of 17:46, 26 October 2013

Our Parts. Fascinating stuff out of the world of non-ribosomal peptides.

Natural Parts

We propose BBa_K1152013 as Best new BioBrick Part, Natural, because:
  • it encodes the original NRPS module from Photorhabdus luminescens capable of synthesizing Indigoidine
  • the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
  • standardization and established protocols allow for BioBrick assembly or Gibson assembly

    Engineered Parts

    We propose BBa_K1152009 as Most Improved Registry Part, because:
  • it encodes for sfp, a 4'-Phosphopanthetheinyl-transferase (PPTase) crucial for the activation of T domains
  • it improved the parts BBa_K802006 and BBa_K302010, which do also encode for sfp from Bacillus subtilis but were deviating in three amino acids from the sequence published on NCBI
  • it is the first BioBrick in the registry that is explicitly encoding sfp, and it is annotated and characterized

    We propose BBa_K1152015, BBa_K1152016, BBa_K1152017, BBa_K1152018, BBa_K1152019, BBa_K1152020 as Best Part Collection, because:
  • they represent a valuable ensemble of various T domains with different properties
  • they were designed semi-rationally based on multiple sequence alignments
  • they exhibit distinct synthesis rates for Indigoidine depending on the T domains of this part collection

    Engineered Devices

    Our favorite part is BBa_K1152007: Helper construct for NRP-Indigoidine-tagging.
  • Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
  • Compatibility with custom non-ribosomal peptide (NRP) synthesis.
  • Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct. We propose BBa_K1152007 as Best new BioBrick Part or Device, Engineered, as mentioned above.

    • List of all submitted parts:

    Thanks to

    Retrieved from "http://2013.igem.org/Team:Heidelberg/Parts"