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Team:Heidelberg/Parts
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Revision as of 15:59, 28 October 2013
Project
Notebook
Human Practice
Our Parts. Now it is Your turn. Start working with NRPS!
Synthetic Peptides - BBa_K1152000 - 2005
We provide the constructs for the novel Dipeptide- and Tripeptide-Synthetases. Furthermore, we submitted the single modules that they are comprised of. These parts should serve as the basis of a future library for standardized work with NRPS. The modules are specific for different amino acids: Phenylalanine (tycA), Proline (tycB1) and Leucine (tycC6).
Indigoidine-Tag - BBa_K1152006 & 2007
One of our major achievements is the establishment of an easily detectable, inert and universal tag - the Indigoidine-Tag. With this tag, purification and validation of novel NRPs is significantly eased. The characteristics of the tag compare to those of the GFP-tag for proteins. We submit the helper-construct for tagging any desired Non-Ribosomal Peptide as our favorite part.
Tag-Optimization - BBa_K1152008 & 2013-2019
Besides establishing the Indigoidine-Tag, we focussed on optimizing its functionality and efficiency. Therefore, we modified the natural sequence of the Indigoidine synthetase indC by domain exchange which lead to altered and in some cases enhanced efficiency of the NRP-production which could be measured by optical density. We submit the collection of alternate T-domains, of which we created several functional, synthetic ones as Best Part Range.
Tag-Characterization - BBa_K1152009 - 2012
During Tag-Optimization, we also focussed on the enzymes that are required for the activation of NRPSs - the PPTases. We characterized several PPTases and assessed their compatibility and efficiency. Furthermore, we improved a part from the registry which is a not annotated PPTase.
List of Parts - BBa_K1152000 - 2019
