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| | Here we will paste some circuit designs | | Here we will paste some circuit designs |
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| - | <b>Enzyme-substrate reactions</b>
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| - | <hr>
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| - | <p>We have cloned fluorescent receiver systems as backup for our circuit in case the hydrolase reaction do not work properly.<br>The enzyme substrate reactions take less than 5 minutes and are visible by eye.<br><br> Our minesweeper become better and better so keep on track for updates !</p>
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| - | <table style="float:left;margin-top:10px">
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| - | <tr>
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| - | <th colspan="2">Hydrolase</th>
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| - | <th colspan="2">Complementary substrate / IUPAC name</th>
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| - | <th>Visible color</th>
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| - | </tr>
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| - | <tr>
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| - | <td>LacZ</td>
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| - | <td>Beta-Galactosidase</td>
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| - | <td>X-Gal</td>
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| - | <td>5-Bromo-4chloro-3-indolyl-beta-galactopyranoside</td>
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| - | <td style="color:blue">Blue</td>
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| - | </tr>
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| - | <tr>
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| - | <td>LacZ</td>
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| - | <td>Beta-Galactosidase</td>
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| - | <td>Green-beta-D-Gal</td>
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| - | <td>N-Methyl-3-indolyl-beta-D_galactopyranoside</td>
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| - | <td style="color:Green">Green</td>
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| - | </tr>
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| - | <tr>
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| - | <td>GusA</td>
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| - | <td>Beta-glucuronidase</td>
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| - | <td>Magenta glucuronide</td>
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| - | <td>6-chloro-3-indolyl-beta-D-glucuronide-cycloheylammonium salt</td>
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| - | <td style="color:Red">Red</td>
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| - | </tr>
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| - | <tr>
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| - | <td>PhoA</td>
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| - | <td>Alkaline phosphatase</td>
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| - | <td>pNPP</td>
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| - | <td>4-Nitrophenylphosphatedi(tris) salt</td>
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| - | <td style="color:Yellow">Yellow</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Aes</td>
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| - | <td>Carboxyl esterase</td>
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| - | <td>Magenta butyrate</td>
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| - | <td>5-bromo-6-chloro-3-indoxyl butyrate</td>
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| - | <td style="color:Magenta">Magenta</td>
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| - | </tr>
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| - | <tr>
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| - | <td>NagZ</td>
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| - | <td>Glycoside hydrolase</td>
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| - | <td>X-glucosaminide X-Glunac</td>
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| - | <td>5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide</td>
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| - | <td style="color:Lightblue">Blue</td>
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| - | </tr>
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| - | </table>
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| - | <br clear="all"/>
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| - | <br><br>
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| - | [[File:Magenta_butyrate_on_aes_t7_strain.JPG|left|148px|thumb|Fig.1: Magenta butyrate on Aes expressing colony]]
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| - | [[File:PNPP_on_phoA_t7_strain.JPG|left|200px|thumb|Fig.2: NPP on PhoA expressing colony]]
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| - | [[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left|180px|thumb|Fig.3 Magenta glucuronide on GusA colony]]
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| - | [[File:XGlcNAc_on_nagZ_t7_strain.JPG|left|200px|thumb|Fig.4: X-Glucnac on NagZ colony]]
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| - | [[File:XGlcNAc_on_nagZ_t7_strain.JPG|left|200px|thumb|Fig.5: X-Gal on LacZ colony]]
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| - | [[File:XGlcNAc_on_nagZ_t7_strain.JPG|left|200px|thumb|Fig.5: Beta-green-X-gal on LacZ colony]]
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| | <br clear="all"/> | | <br clear="all"/> |
Tranformation and cloning constructs
| Sender cells |
Receiver cells |
| AHL expressing system |
Fluorescent reporter system |
Enzyme reaction-based reporter system |
| Pcons-LuxI 1 (Strong) |
Plac-LuxR-Plux-GFP |
Plac-LuxR-Plux-LacZ |
| Pcons-LuxI 2 |
Plac-LuxR-Plux-YFP |
Plac-LuxR-Plux-Aes |
| Pcons-LuxI 3 |
Plac-LuxR-Plux-CFP |
Plac-LuxR-Plux-GusA |
| Pcons-LuxI 4 (Weak) |
|
Plac-LuxR-Plux-PhoA |
|
|
Plac-LuxR-Plux-NagZ |
| Biobrick |
Registry part |
| LuxI |
K805016 |
| LuxR |
C0062 |
| Pcons 1 (Strong) |
J23100 |
| Pcons 2 |
J23118 |
| Pcons 3 |
J23110 |
| Pcons 4 (Weak) |
J23114 |
Mutagenized Plux
( low [AHL]) |
Optimized Biobrick ! |
Mutagenized Plux
( middle [AHL]) |
Optimized Biobrick ! |
Mutagenized Plux
( high [AHL]) |
Optimized Biobrick ! |
| pLuxR |
R0062 |
| Plac |
I14032 |
| GFP |
E0840 |
| YFP |
E0030 |
| CFP |
E0020 |
| LacZ |
I732006 |
GusA |
New Biobrick ! |
| PhoA |
New Biobrick ! |
| Aes |
New Biobrick ! |
| NagZ |
New Biobrick ! |
Here we will paste some circuit designs
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