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Team:ETH Zurich/Experiments
From 2013.igem.org
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| + | <p>For the final Colisweeper circuit we plan a four plasmid system. The mine cells constitutively express LuxI and NagZ. In the Non-Mine cells LuxR and PhoA are expressed constitutively whereas Aes and GusA are expressed from pLux promoters with different sensitivities. To get to this system we tested different versions of the circuit with GFP. In the following table we list all the plasmids we cloned, which biobricks we used and what experiments we used them for. </p> | ||
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| + | [[File:Plasmidmap.png|1050px|left|thumb|<b>Figure 1. Transformed plasmids for mine cells and non-mine cells </b>]] | ||
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Revision as of 17:43, 19 September 2013
Cloned constructs
For the final Colisweeper circuit we plan a four plasmid system. The mine cells constitutively express LuxI and NagZ. In the Non-Mine cells LuxR and PhoA are expressed constitutively whereas Aes and GusA are expressed from pLux promoters with different sensitivities. To get to this system we tested different versions of the circuit with GFP. In the following table we list all the plasmids we cloned, which biobricks we used and what experiments we used them for.
| Sender cells / OHHL expressing systems |
|---|
| Pcons-LuxI (1) (Strong) |
| Pcons-LuxI (2) |
| Pcons-LuxI (3) |
| Pcons-LuxI (4) (Weak) |
| Receiver cells / OHHL detecting and processing systems | |
|---|---|
| Fluorescent reporter system | Enzyme reaction-based reporter system |
| PJ23100-LuxR-PluxR-GFP | PJ23100-LuxR-PluxR-LacZ |
| PJ23100-LuxR-PluxR-YFP | PJ23100-LuxR-PluxR-Aes |
| PJ23100-LuxR-PluxR-CFP | PJ23100-LuxR-PluxR-GusA |
| PJ23100-LuxR-PluxR-PhoA | |
| PJ23100-LuxR-PluxR-NagZ |
