Team:Heidelberg/Parts

From 2013.igem.org

(Difference between revisions)
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<li> Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
<li> Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
<br><br>
<br><br>
 +
We propose <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a> as Best new BioBrick Part, Natural, because:
We propose <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a> as Best new BioBrick Part, Natural, because:
-
<li> Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
+
<li> it encodes the original NRPS module from ''Photorhabdus luminescens'' capable of synthesizing Indigoidine
-
<li> Compatibility with custom non-ribosomal peptide (NRP) synthesis.
+
<li> the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
-
<li> Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
+
<li> standardization and established protocols allow for BioBrick assembly or Gibson assembly
-
<li> Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
+
 
-
<li> Compatibility with custom non-ribosomal peptide (NRP) synthesis.
+
<br><br>
-
<li> Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
+
We propose <a href="http://parts.igem.org/Part:BBa_K1152007">BBa_K1152007</a> as Best new BioBrick Part or Device, Engineered, as mentioned above.
 +
 
 +
We propose <a href="http://parts.igem.org/Part:BBa_K1152009">BBa_K1152009</a> as Most Improved Registry Part, because:
 +
<li> it encodes the original NRPS module from ''Photorhabdus luminescens'' capable of synthesizing Indigoidine
 +
<li> the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
 +
<li> standardization and established protocols allow for BioBrick assembly or Gibson assembly

Revision as of 02:27, 5 October 2013

Our Parts. Fascinating stuff out of the world of non-ribosomal peptides.

Team Parts

Our favorite part is BBa_K1152007: Helper construct for NRP-Indigoidine-tagging.
  • Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
  • Compatibility with custom non-ribosomal peptide (NRP) synthesis.
  • Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.

    We propose BBa_K1152013 as Best new BioBrick Part, Natural, because:
  • it encodes the original NRPS module from ''Photorhabdus luminescens'' capable of synthesizing Indigoidine
  • the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
  • standardization and established protocols allow for BioBrick assembly or Gibson assembly

    We propose BBa_K1152007 as Best new BioBrick Part or Device, Engineered, as mentioned above. We propose BBa_K1152009 as Most Improved Registry Part, because:
  • it encodes the original NRPS module from ''Photorhabdus luminescens'' capable of synthesizing Indigoidine
  • the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
  • standardization and established protocols allow for BioBrick assembly or Gibson assembly

    • Thanks to

    Retrieved from "http://2013.igem.org/Team:Heidelberg/Parts"