Team:Heidelberg/Project/Indigoidine

From 2013.igem.org

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The small molecule produced by the pathway described above is a blue-colored pigment called <em>indigoidine</em>. Accordingly, the catalytic NRPS is referred to as <em>indigoidine synthetase</em> or <em>blue pigment synthetase</em> encoded by various bacterial strains such as <em>S. lavendulae subsp. lavendulae</em> (ATCC11924) or <em>P. luminescens</em> (<bib id="Takahashi2007"/><bib id="Brachmann2012"/>). Previous publications showed that replacing the T-domain of the blue pigment synthetase bpsA from <em>S. lavendulae</em> with T-domains of other NRPS modules results in a loss of function, i.e. the engineered indigoidine synthetase does not produce the blue pigment (<bib id="Owen2012"/). So far the only T-domain exchange that resulted in a functional bpsA was achieved using the T-domain of entF, - a gene involved in the enterobactin biosynthesis of <em>E. coli</em>. For this approach, random mutagenesis was performed to yield various mutated entF T-domains, some of which were capable to preserve the enzyme function when being introduced into bpsA (<bib id="Owen2012"/>). Other studies revealed that it is possible to exchange the A-domains of NRPS modules in <em>B. subtilis</em> resulting in modified non-ribosomal peptide products (<bib id="Doekel2000"/> ). Additionally, the selectivity of an NRPS module for specific amino acids could be modified by altering the conserved motif in the active site of the A-domain (<bib id="Thirlway2012"/>). Furthermore, since the endogenous 4'-Phoshopanthetheinyl-transferase (PPTase) entD from <em>E. coli</em> has been reported to exhibit low efficiency in activating heterologous NRPS pathways, most research in the field of NRPS involves co-expression of another PPTase (<bib id="Pfeifer2001"/>), bib id="Takahashi2007"/>).
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The small molecule produced by the pathway described above is a blue-colored pigment called <em>indigoidine</em>. Accordingly, the catalytic NRPS is referred to as <em>indigoidine synthetase</em> or <em>blue pigment synthetase</em> encoded by various bacterial strains such as <em>S. lavendulae subsp. lavendulae</em> (ATCC11924) or <em>P. luminescens</em> (<bib id="Takahashi2007"/><bib id="Brachmann2012"/>). Previous publications showed that replacing the T-domain of the blue pigment synthetase bpsA from <em>S. lavendulae</em> with T-domains of other NRPS modules results in a loss of function, i.e. the engineered indigoidine synthetase does not produce the blue pigment (<bib id="Owen2012"/>). So far the only T-domain exchange that resulted in a functional bpsA was achieved using the T-domain of entF - a gene involved in the enterobactin biosynthesis of <em>E. coli</em>. For this approach, random mutagenesis was performed to yield various mutated entF T-domains, some of which were capable to preserve the enzyme function when being introduced into bpsA (<bib id="Owen2012"/>). Other studies revealed that it is possible to exchange the A-domains of NRPS modules in <em>B. subtilis</em> resulting in modified non-ribosomal peptide products (<bib id="Doekel2000"/> ). Additionally, the selectivity of an NRPS module for specific amino acids could be modified by altering the conserved motif in the active site of the A-domain (<bib id="Thirlway2012"/>). Furthermore, since the endogenous 4'-Phoshopanthetheinyl-transferase (PPTase) entD from <em>E. coli</em> has been reported to exhibit low efficiency in activating heterologous NRPS pathways, most research in the field of NRPS involves co-expression of another PPTase (<bib id="Pfeifer2001"/>), bib id="Takahashi2007"/>).
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<h2 id="aim">Aim</h2>
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We want to find the best approach to determine the linker structure between the A-, T- and TE-domain of indC by exchanging the native T-domain with the T-domain of bpsA.
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We want to proof that it is possible to exchange the indC T-domain with T-domains from other NRPS modules that are not related to indigoidine synthetases and/or the <em>P. luminescens</em> strain
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We want to point out the great potential of NRPS for synthetic approaches by designing synthetic T-domains that will result in a functional indigoidine synthetase when introduced to indC
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We want to show that the activity of NRPS is dependant on the PPTase used to activate them and that the right combination of PPTase and T-domain plays a crucial role when trying to increase the overall product yield.
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We want to exhibit the potential of NRPS modularity by converting native NRPS modules with glutamine specificity into an indigoidine synthetase by introducing an oxidation domain into the Adenylation-domain. Additionally we will try out various domain combinations to assemble and configure a indigoidine synthetase comprised of different NRPS modules.
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<h2 id="results">Results </h2>
<h2 id="results">Results </h2>
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<h3 id="claims">Engineered indigoidine synthetases retain functionality</h3>
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<h3>Expression of functional indigoidine synthetase indC derived from ''P. lumninescens'' in five substrains of ''E. coli''</h3>
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We successfully engineered the nonribosomal peptide synthetase indC from <em>P. luminescens subsp. Laumondii</em> TT01 by replacing its native T-domain with both T-domains of other NRPS modules from different bacterial strains and synthetic T-domains of own design, thus creating a library of 58 engineered variants of the indC indigoidine synthetase. So far only the exchange of the T-domain in the related indigoidine synthetase bpsA from <em>S. lavendulae</em> ATCC11924 with the T-domain of the <em>E. coli</em> entF NRPS module was reported, postulating that the T-domain cannot be replaced by other native T-domains retaining the overall protein function, since the engineered variants of bpsA were incapable of producing the blue pigment indigoidine (<bib id="Owen2012"/>). <br/>
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The open reading frame of the native indigoidine synthetase indC was amplified from genomic DNA of ''P. lumninescens'' and cloned into a plasmid under the control of an lac-inducible promoter. This indC expression cassette was transformed into different substrains of ''E. coli'', namely DH5alpha, MG1655, BAP1, TOP10 and NEB Turbo. All of these host strains express the endogenous PPTAse entD which is responsible for the transfer of the 4'-phosphopantetheine residue from coenzyme A to the apo-domain of EntF, a T-domain in the enterobactin pathway(<bib id="pmid:9214294"/>). As depicted in Figure 6a, entD does not exhibit strict substrate speceficity in being restricted to activating domains of the enterobactin pathway, but is able to activate the T-domain of indC as determined by the blue phenotype of the transformed cells. Except for NEB Turbo cells, all transformed host strains displayed a decelerated growth and significantly smaller colonies on plate when compared to the negative control. The blue phenotype developed late after transformation ranging from first blue colonies after 24 h and taking up to three days for visible poduction of the blue pigment. NEB Turbo showed regular colony growth and developed a strong blue phenotype upon induction with IPTG. As all host strains were able to express the functional indigoidine sythetase derived from a different species, further experiments were only conducted with one ''E. coli'' strain. Due to its simplicity in handling and sufficient expression of the constructs, the substrain TOP10 was chosen.
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However, our data shows that the T-domain of indC can be replaced by the T-domain of bpsA only if specific T-domain border combinations are used (see Fig. x) to define the replaced fragment (Figure xa). We proved that indC retained its functionality when the T-domain of the native indC gene was replaced with T-domains of other NRPS modules (see Figure X), applying the T-domain border combination "A2" (see Fig. x). We also tried the T-domain border combination "B2" with the same T-domains, as these T-domain borders were proposed by the Pfam domain prediction tool (pfam.sanger.ac.uk). In this approach less transformants were capable of producing the plue pigment (data not shown), suggesting that the T-domain border combination "A2" can be seen as an improved variant compared to the Pfam prediction.
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<h3>Improved Production of Indigoidine by Co-transformation of Host Strain with Supplementary PPTases</h3>
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The expression of indC under activation by endC was sufficient for easy detection of indigoidine production on plates harboring indC-carrying cells. In order to determine, whether the amount of indigoidine production in the ''E. coli''TOP10 cells is dependent on the quality of the interacting of indC with the PPTase, four PPTase dervied from varying origins were selected and amplified from the genome of the hosts of origin. ''E. coli''TOP10 cells were co-transformed with plasmids coding for the different PPTases and the plasmid containing the expression cassette for indC. As reference for the endogenous PPTase activity served cells only transformed with the indC plasmid. Irrespective of the PPTase, growth of colonies was retarded. Remarkably however, colonies co-transformed with the PPTase plasmid remained of smaller size than the ones only carrying the indC construct. On the other side, indigoidine production was more diffuse in the latter cells with secretion of the blue pigment into the agar (Figure 6b, indC) and only slight blue-greenish coloring of the colonies. The four PPTases additionally introduced into the TOP10 cells were all shown to be functional (blue phenotype of the transformants, Figure 6b), but lead to the retention of most of the indigoidine within the cells. Colonies of cells transformed with thess constructs, were of convex shape and of distinct, dark blue color. Overall, cells carrying an additional PPTase showed increased indigoidine production compared to the cells relying on the endogenous entD.
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<h3>Synthetic T-Domains Generated by Consensus and Guided Random Design Method are Functional</h3>
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The main structurel characteristic of NRPSs is their modular composition on different levels. The indogoidine synthetase indC is a one-module NRPS comprised of the three domains, namely AoxA, T and TE. Since the functionality of this NRPS is detectable by the bare eye, it offers a perfect and simple experimental set-up for proof of principle experiments regarding the interchangeability of domains from different NRPS. We choose a two-pronged domain shuffling approach: First, we transferred native T-domains derived from either different host species and/or NRPSs of entirely different function into the indC indigoidine synthetase. Second, we deviced three methods for the generation of synthetic T-domains based on different NRPS libraries generated by BLAST search against either specific subranges of host organisms or restricting the query sequence to be BLASTed.(link) For the libraries, where the query sequence was restricted to the T-domain of indC, prior knowledge about the T-domain boundaries was required. We used PFAM, a web-tool which allows -amongst other functions- for the prediction of NRPS's module and domain boundaries, to define the T-domain of indC.
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As depicted in Figure 7, both approaches lead principally to fully functional indCs. The synthetic T-domains 1, 3 and 4 showed the same retarded growth and indigoidin production on plates as did the native T-domain derived from ''P. lumninescens''. The colonies obtained after co-transformation with supplementary PPTase plasmid were small in size and of dark blue color. Compared to synthetic T-domain 5, indigoidin production started earlier (approximately after 24-30 hours). In contrast to the synthetic domains 1,3 and 4 which were designed by the consensus method and showed medium to high similarity to the sequence of origin, synthetic domain 5 was generated by the guided random method. Remarkably, even though 39 out of the 62 amino acids of the original T-domain were exchange, the indigoidine synthetase with this T-domain was still functional. Closer analysis of the sequence compared to the original indC T-domain sequence  showed, that the characteristics of the amino acid sequence, i. e. for instance polar or charged amino acids, were retained in 72% of the sequence. Also, the GGXS core sequence of the T-domain at which the activation by the PPTas occurs was conserved.
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<h3>Domain Shuffling Works across Modules Derived from different Pathways and host organisms</h3>
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Multiple web-tools exist which offer the prediction of NRPS module and domain boundaries. One of the most common used prediction tools is PFAM which we used as a starting point to determine the best method for defining domain boundaries. PFAM predicted large linker structures between the end of the A and the beginning of the T-domain. Using these domain boundaries for the native T-domains did only yield one functional native T-domain. We tried to improve this yield by defining new T-domain boundaries based on the predictions of PFAM and multiple sequence alingments with the respective homology libraris at the predicted linker regions. Boundaries were set closer to the preceeding A-domain, at regions were less sequence conservation was observed. Figure 9 depicts the success of this strategy as two additional native T-domains derived from delH4 and bpsA (indigoidine synthetase) led to functional indCs and the production of indigoidine. In addition, the native T-domain from plu2642 which was already shown to be functional(compare Figure 7) showed faster and increased indigoidine production (deep blue agar plate, lower right panel on Figure 9). The results obtained from this experiments proofed two concepts. First, domain shuffling is possible across different species as the T-domains of delH4 and bpsA were derived from ''D. acidovorans'' and ''S. lavendulae lavendulae'', respectively and were functional in ''E. coli''. Also, shuffling of domains from modules of different substrate specificity has been proofen herein. Second,  manually adjusting the boundaries predicted by PFAM based on MSA is a functional method to predict functional T-domain boundaries.
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<h2 id="results">Discussion</h2>
<h2 id="results">Discussion</h2>
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As model system, we used the unimodular indigoidine synthetase NRPS from <em>P. luminescens subsp. Laumondii</em> TT01. We predicted the modular composition and domain borders of IndC using our own NRPS-Designer software.
As model system, we used the unimodular indigoidine synthetase NRPS from <em>P. luminescens subsp. Laumondii</em> TT01. We predicted the modular composition and domain borders of IndC using our own NRPS-Designer software.
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We then started by replacing the native IndC T-domain with T-domains derived from different NRPS pathways from different bacterial strains, among those the T-domain from the BpsA indigoidine synthetase from <em>S. lavendulae<em> ATCC11924. Constructs were transformed into E. coli alongside with an PPTase expression cassette in order to screen for functional IndC variants. As hoped, a subset of the natural T-domains were functioning in context of the IndC scaffold module, leading to indigoidine production and thereby blue coloring of colonies and corresponding liquid cultures. We then engineered a variety of synthetic T-domains derived from consensus sequences of different natural T-domains. Again, a subset of these T-domains successfully maintained indigoidine production (<a class="fancybox fancyFigure" title="Figure 3" href="https://static.igem.org/mediawiki/2013/6/6e/Heidelberg_IndPD_Fig9.png" rel="gallery1">Fig. 9</a>).
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We then started by replacing the native IndC T-domain with T-domains derived from different NRPS pathways from different bacterial strains, among those the T-domain from the BpsA indigoidine synthetase from <em>S. lavendulae</em> ATCC11924. Constructs were transformed into E. coli alongside with an PPTase expression cassette in order to screen for functional IndC variants. As hoped, a subset of the natural T-domains were functioning in context of the IndC scaffold module, leading to indigoidine production and thereby blue coloring of colonies and corresponding liquid cultures. We then engineered a variety of synthetic T-domains derived from consensus sequences of different natural T-domains. Again, a subset of these T-domains successfully maintained indigoidine production (<a class="fancybox fancyFigure" title="Figure 9" href="https://static.igem.org/mediawiki/2013/6/6e/Heidelberg_IndPD_Fig9.png" rel="gallery1">Fig. 9</a>).
Notably, one of our engineered IndC construcats showed an indigoidine production even higher compared to the wild-type IndC (T-domain Plu2642; <a class="fancybox fancyFigure" title="Figure 3" href="https://static.igem.org/mediawiki/2013/6/6c/Heidelberg_IndPD_Fig3.png" rel="gallery1">Figure 3</a>).
Notably, one of our engineered IndC construcats showed an indigoidine production even higher compared to the wild-type IndC (T-domain Plu2642; <a class="fancybox fancyFigure" title="Figure 3" href="https://static.igem.org/mediawiki/2013/6/6c/Heidelberg_IndPD_Fig3.png" rel="gallery1">Figure 3</a>).
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     <img style="width:60%; margin-bottom:10px; padding:1%;border-style:solid;border-width:1px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/c/cf/Heidelberg_IndPD_Fig6.png"></img>
     <img style="width:60%; margin-bottom:10px; padding:1%;border-style:solid;border-width:1px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/c/cf/Heidelberg_IndPD_Fig6.png"></img>
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     <figcaption><b>Figure 6</b>:  
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     <figcaption><b>Figure 6</b>: Comparison between different <em>E. coli</em> strains and PPTases:
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    a) Comparison of different <em>E. coli</em> strains examining growth and indigoidine production
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The figure shows five different strains of <em>E. coli</em> that have been co-transformed with an indC expression plasmid and a sfp expression plasmid. The negative control is <em>E. coli</em> TOP10 without a plasmid. All transformants have been grown on LB agar for 48 hours at room temperature, cells were not induced. One can see that even without induction all strains express the indigoidine synthetase and produce the blue pigment indigoidine. However, the strains BAP1 and NEB Turbo grow faster in the first day, exhibiting a white phenotype (data not shown). Colonies on the plate of <em>E. coli</em> TOP10  are very small and dark blue/ black. Assuming that indigoidine production inhibits cell growth due to its toxicity, we concluded that TOP10 produced the most indigoidine among the strains we tested. We used <em>E. coli</em> TOP10 for the following experiments.
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b) Comparison between different PPTases concerning overall indigoidine production
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The Figure shows ''E. coli'' TOP10 cells co-transformed with indC and four different PPTases (sfp, svp, entD and delC), respectively. The image bottom left shows ''E. coli'' TOP10 cells without additional PPTase and the negative control is TOP10 without a plasmid.
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                   <a class="fancybox fancyGraphical" href="https://static.igem.org/mediawiki/2013/f/f1/Heidelberg_IndPD_Fig7.png">
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     <img style="width:60%; margin-bottom:10px; padding:1%;border-style:solid;border-width:1px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/f/f1/Heidelberg_IndPD_Fig7.png"></img>
     <img style="width:60%; margin-bottom:10px; padding:1%;border-style:solid;border-width:1px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/f/f1/Heidelberg_IndPD_Fig7.png"></img>
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     <figcaption><b>Figure 7</b>:
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     <figcaption><b>Figure 7: Modified variants of indC with replaced T-domains</b>
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    We replaced the indC T-domain with both the T-domains of other native NRPS modules (entF, delH, tycC, tycA, bpsA, plu2642 and plu2670) and synthetic T-domains. The figure shows the five modified versions of indC that remain the enzyme function, thus resulting in a blue phenotype of transformed ''E. coli'' TOP10. The cells have been co-transformed with a plasmid containing the respective engineered variant of indC and a second plasmid coding for the PPTase sfp, svp, entD and delC. The figure shows representative results; the total 85 transformation results can be found in the indigoidine notebook, week 17.
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     <img style="width:60%; margin-bottom:10px; padding:1%;border-style:solid;border-width:1px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/e/eb/Heidelberg_IndPD_Fig8.png"></img>
     <img style="width:60%; margin-bottom:10px; padding:1%;border-style:solid;border-width:1px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/e/eb/Heidelberg_IndPD_Fig8.png"></img>
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     <figcaption><b>Figure 8</b>:  
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     <figcaption><b>Figure 8: Determination of required domain borders for T-domain exchange</b>
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    a) Definition of different domain border combinations for T-domain exchanges
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The figure shows a sequence alignment of the indC and bpsA amino acid sequences.
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The alignment was created using clustalO (http://www.ebi.ac.uk/Tools/msa/clustalo/) with
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standard parameters. The lines marked A, B and C reflect the borders we used between the A-
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and the T-domain, whereas those marked, 1, 2, 3 and 4 reflect the borders between the T- and
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the TE-domain. In total we tried all twelve combinations of a domain border {A, B, C} and a
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domain border {1, 2, 3, 4}, replacing the sequence inbetween with the respective part of bpsA.
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b) E. coli TOP10 co-transformed with modified versions of indC and the PPTase sfp
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The co-tranformation of the modified indC-(bpsA-T) plasmids described above with a second
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plasmid coding for the PPTase Sfp shows that only three domain border combinations can be
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used for exchanging the indC T-domain with the T-domain of bpsA. These are the
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combinations A1, A2 and C1. We applied combination A2 for further T-domain exchanges.
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     <img style="width:60%; margin-bottom:10px; padding:1%;border-style:solid;border-width:1px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/6/6e/Heidelberg_IndPD_Fig9.png"></img>
     <img style="width:60%; margin-bottom:10px; padding:1%;border-style:solid;border-width:1px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/6/6e/Heidelberg_IndPD_Fig9.png"></img>
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     <figcaption><b>Figure 9</b>:  
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     <figcaption><b>Figure 9: Applying optimized domain border combinations by T-domain exchange of native NRPS T-domains</b>
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    As described above, we determined an optimized domain border combination for the exchange of the native indC T-domain with the T-domain of the indigoidine synthetase bpsA. We applied this border combination (previously referred to as ''A2'') to the T-domains of the NRPS modules entF, delH4, delH5, tycA1, tycC6, plu2642 and plu2670, replacing the indC T-domain with the respective fragments of those modules. This figure shows three transformants with a blue phenotype in which the indC T-domain was exchanged by the T-domain of the respective NRPS module. The pictures were taken after 60 hours of incubation at room temperature. Once more one can see the differences in growth kinetics due to the production of indigoidine: Cells expressing the indC variant with the T-domain of bpsA  grow very slow and form small and dark blue colonies, whereas cells expressing other variants grow faster. Comparing the images on the very right, we suggest that cells expressing indC with the T-domain of plu2642 produce the most indigoidine in the given timeframe, compared to both the delH4- and the bpsA-variant. The combination of the plu2642 T-domain and the indC indigoidine synthetase seems to be ideal, concerning both indigoidine production and overall growth.
     </figcaption>
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Revision as of 02:32, 5 October 2013

Indigoidine. Proving Modularity of NRPS by Shuffling Domains.

Highlights

Abstract

An integral characteristic of synthetic biology yet often undermined is the ability to learn fundamental knowledge by systematically perturbing a biological system. Non-ribosomal peptide synthetases (NRPS) are predestinated for such a trial and error approach. Their hierarchical organization into modules and domains offer a unique opportunity to spin around their inherent logical assembly and observe if their functionality is preserved. Following this idea, we prove the interchangeability of NRPS domains at the example of indC from Photorhabdus luminescens laumondii TT01 (DSM15139). The native NRPS domains have been replaced with domains from other bacterial organisms and fully synthetic domains. To quantify the NRPS efficiency we established an indigoidine assay based on OD measurement of the blue-colored pigment. Interestingly, we find that our data points out the dependence on the T-domain and the 4'-Phosphopanthetheinyl-transferases (PPTases), resulting in different levels of indigoidine synthesis. Furthermore, we introduce HiCT - High throughput protocols for circular polymerase extension Cloning and Transformation - a new standard for the assembly of combinatorial gene libraries (RFC 99).

Introduction

Most modules of non-ribosomal peptide synthetase (NRPS) pathways consist of three domain types: condensation, adenylation and thiolation domain (see Figure 1a), also called peptidyl-carrier-protein domain (PCP)-domain (reviewed in )). Remarkably, precisely this order of domains is highly conserved among different NRPS pathways except for the very first and last module of each NRPS. In the initial module, the A-domain is always followed by a T-domain. The last module of an NRPS usually ends with a TE-domain. During the process of non-ribisomal peptide (NRP) synthesis, a new amino acid is first adenylated by the A-domain and then bound to the T-domain via a thioester bond. The C-domain catalyzes the condensation of the substrate - which is bound to the T-domain of the previous module - and the amino acid of the next module. The T-domain itself shows no substrate specificity but acts as a carrier domain, which keeps the peptide attached to the NRPS module complex. The core element of every T-domain is a conserved 4’-phosphopanthetheinylated (4’-PPT) serine. The 4’-PPT residue is added by a 4’-Phosphopanthetheinyl-transferase (PPTase), which converts the NRPS apo-enzyme to its active holo-form (see Figure 1b).

Figure 1: NRPS module and domain structure and activation of T-domains. a) Basic structure of NRPS pathways Typically, a NRPS is composed of 1 to about 10 single modules, of which each consists of a C-domain (condensation domain), an A-domain (adenylation domain) and a T-domain (thiolation domain). Moreover, the initial module lacks the C-domain and the last module of a pathway has an additional TE-domain (thioesterase domain), which cleaves the synthesized nonribosomal peptide from the last T-domain. b) Activation of NRPS modules by 4'-Phosphopanthetheinylation of the T-domain. Every NRPS module has to be activated by a 4'-Phosphopanthetheinyl-transferase (PPTase). which transfers the 4'-Phosphopanthetheinyl moiety of Coenzyme A to a conserved serine residue in the T-domain.

Besides these fundamental domains (C-domain, A-domain and T-domain), some NRPS modules incorporate additional domains enlarging the amount of potential catalytic reactions, such as cyclization, epimerization or oxidation of the amino acid [Reference].
For example, a single module of P. luminescens laumondii TT01 (DSM15139) contains an internal oxidation domain (Ox-domain) in its A-domain and a special TE-domain (Figure 2a). This enzyme first adenylates L-glutamine (A-domain), which is then attached to the T-domain. The TE-domain cleaves and catalyzes the cyclization of the substrate, which is further oxidized by the Ox-domain. The oxidation of two cyclic glutamines results in the formation of an insoluble small molecule (Figure 2b)[Reference].

Figure 2: Exchange of the indC T-domain on a pSB1C3 derived expression plasmid The indigoidine synthetase indC from P. luminescens consists of an adenylation domain with an internal oxidation domain, a thiolation domain and a thioesterase domain. We replaced the indC T-domain with T-domains of other NRPS modules (one of which is the indigoidine synthetase bpsA from S. lavendulae) and seven synthetic T-domains, which were customized to be introduced to indC, respectively.
The small molecule produced by the pathway described above is a blue-colored pigment called indigoidine. Accordingly, the catalytic NRPS is referred to as indigoidine synthetase or blue pigment synthetase encoded by various bacterial strains such as S. lavendulae subsp. lavendulae (ATCC11924) or P. luminescens (). Previous publications showed that replacing the T-domain of the blue pigment synthetase bpsA from S. lavendulae with T-domains of other NRPS modules results in a loss of function, i.e. the engineered indigoidine synthetase does not produce the blue pigment (). So far the only T-domain exchange that resulted in a functional bpsA was achieved using the T-domain of entF - a gene involved in the enterobactin biosynthesis of E. coli. For this approach, random mutagenesis was performed to yield various mutated entF T-domains, some of which were capable to preserve the enzyme function when being introduced into bpsA (). Other studies revealed that it is possible to exchange the A-domains of NRPS modules in B. subtilis resulting in modified non-ribosomal peptide products ( ). Additionally, the selectivity of an NRPS module for specific amino acids could be modified by altering the conserved motif in the active site of the A-domain (). Furthermore, since the endogenous 4'-Phoshopanthetheinyl-transferase (PPTase) entD from E. coli has been reported to exhibit low efficiency in activating heterologous NRPS pathways, most research in the field of NRPS involves co-expression of another PPTase (), bib id="Takahashi2007"/>).

Results

Expression of functional indigoidine synthetase indC derived from ''P. lumninescens'' in five substrains of ''E. coli''

The open reading frame of the native indigoidine synthetase indC was amplified from genomic DNA of ''P. lumninescens'' and cloned into a plasmid under the control of an lac-inducible promoter. This indC expression cassette was transformed into different substrains of ''E. coli'', namely DH5alpha, MG1655, BAP1, TOP10 and NEB Turbo. All of these host strains express the endogenous PPTAse entD which is responsible for the transfer of the 4'-phosphopantetheine residue from coenzyme A to the apo-domain of EntF, a T-domain in the enterobactin pathway(). As depicted in Figure 6a, entD does not exhibit strict substrate speceficity in being restricted to activating domains of the enterobactin pathway, but is able to activate the T-domain of indC as determined by the blue phenotype of the transformed cells. Except for NEB Turbo cells, all transformed host strains displayed a decelerated growth and significantly smaller colonies on plate when compared to the negative control. The blue phenotype developed late after transformation ranging from first blue colonies after 24 h and taking up to three days for visible poduction of the blue pigment. NEB Turbo showed regular colony growth and developed a strong blue phenotype upon induction with IPTG. As all host strains were able to express the functional indigoidine sythetase derived from a different species, further experiments were only conducted with one ''E. coli'' strain. Due to its simplicity in handling and sufficient expression of the constructs, the substrain TOP10 was chosen.
Figure 3:

Improved Production of Indigoidine by Co-transformation of Host Strain with Supplementary PPTases

The expression of indC under activation by endC was sufficient for easy detection of indigoidine production on plates harboring indC-carrying cells. In order to determine, whether the amount of indigoidine production in the ''E. coli''TOP10 cells is dependent on the quality of the interacting of indC with the PPTase, four PPTase dervied from varying origins were selected and amplified from the genome of the hosts of origin. ''E. coli''TOP10 cells were co-transformed with plasmids coding for the different PPTases and the plasmid containing the expression cassette for indC. As reference for the endogenous PPTase activity served cells only transformed with the indC plasmid. Irrespective of the PPTase, growth of colonies was retarded. Remarkably however, colonies co-transformed with the PPTase plasmid remained of smaller size than the ones only carrying the indC construct. On the other side, indigoidine production was more diffuse in the latter cells with secretion of the blue pigment into the agar (Figure 6b, indC) and only slight blue-greenish coloring of the colonies. The four PPTases additionally introduced into the TOP10 cells were all shown to be functional (blue phenotype of the transformants, Figure 6b), but lead to the retention of most of the indigoidine within the cells. Colonies of cells transformed with thess constructs, were of convex shape and of distinct, dark blue color. Overall, cells carrying an additional PPTase showed increased indigoidine production compared to the cells relying on the endogenous entD.

Synthetic T-Domains Generated by Consensus and Guided Random Design Method are Functional

The main structurel characteristic of NRPSs is their modular composition on different levels. The indogoidine synthetase indC is a one-module NRPS comprised of the three domains, namely AoxA, T and TE. Since the functionality of this NRPS is detectable by the bare eye, it offers a perfect and simple experimental set-up for proof of principle experiments regarding the interchangeability of domains from different NRPS. We choose a two-pronged domain shuffling approach: First, we transferred native T-domains derived from either different host species and/or NRPSs of entirely different function into the indC indigoidine synthetase. Second, we deviced three methods for the generation of synthetic T-domains based on different NRPS libraries generated by BLAST search against either specific subranges of host organisms or restricting the query sequence to be BLASTed.(link) For the libraries, where the query sequence was restricted to the T-domain of indC, prior knowledge about the T-domain boundaries was required. We used PFAM, a web-tool which allows -amongst other functions- for the prediction of NRPS's module and domain boundaries, to define the T-domain of indC. As depicted in Figure 7, both approaches lead principally to fully functional indCs. The synthetic T-domains 1, 3 and 4 showed the same retarded growth and indigoidin production on plates as did the native T-domain derived from ''P. lumninescens''. The colonies obtained after co-transformation with supplementary PPTase plasmid were small in size and of dark blue color. Compared to synthetic T-domain 5, indigoidin production started earlier (approximately after 24-30 hours). In contrast to the synthetic domains 1,3 and 4 which were designed by the consensus method and showed medium to high similarity to the sequence of origin, synthetic domain 5 was generated by the guided random method. Remarkably, even though 39 out of the 62 amino acids of the original T-domain were exchange, the indigoidine synthetase with this T-domain was still functional. Closer analysis of the sequence compared to the original indC T-domain sequence showed, that the characteristics of the amino acid sequence, i. e. for instance polar or charged amino acids, were retained in 72% of the sequence. Also, the GGXS core sequence of the T-domain at which the activation by the PPTas occurs was conserved.

Domain Shuffling Works across Modules Derived from different Pathways and host organisms

Multiple web-tools exist which offer the prediction of NRPS module and domain boundaries. One of the most common used prediction tools is PFAM which we used as a starting point to determine the best method for defining domain boundaries. PFAM predicted large linker structures between the end of the A and the beginning of the T-domain. Using these domain boundaries for the native T-domains did only yield one functional native T-domain. We tried to improve this yield by defining new T-domain boundaries based on the predictions of PFAM and multiple sequence alingments with the respective homology libraris at the predicted linker regions. Boundaries were set closer to the preceeding A-domain, at regions were less sequence conservation was observed. Figure 9 depicts the success of this strategy as two additional native T-domains derived from delH4 and bpsA (indigoidine synthetase) led to functional indCs and the production of indigoidine. In addition, the native T-domain from plu2642 which was already shown to be functional(compare Figure 7) showed faster and increased indigoidine production (deep blue agar plate, lower right panel on Figure 9). The results obtained from this experiments proofed two concepts. First, domain shuffling is possible across different species as the T-domains of delH4 and bpsA were derived from ''D. acidovorans'' and ''S. lavendulae lavendulae'', respectively and were functional in ''E. coli''. Also, shuffling of domains from modules of different substrate specificity has been proofen herein. Second, manually adjusting the boundaries predicted by PFAM based on MSA is a functional method to predict functional T-domain boundaries.

Discussion

In this subproject, we wanted to set the basis for engineering entirely synthetic NRPS modules composed of user-defined domains. As model system, we used the unimodular indigoidine synthetase NRPS from P. luminescens subsp. Laumondii TT01. We predicted the modular composition and domain borders of IndC using our own NRPS-Designer software. We then started by replacing the native IndC T-domain with T-domains derived from different NRPS pathways from different bacterial strains, among those the T-domain from the BpsA indigoidine synthetase from S. lavendulae ATCC11924. Constructs were transformed into E. coli alongside with an PPTase expression cassette in order to screen for functional IndC variants. As hoped, a subset of the natural T-domains were functioning in context of the IndC scaffold module, leading to indigoidine production and thereby blue coloring of colonies and corresponding liquid cultures. We then engineered a variety of synthetic T-domains derived from consensus sequences of different natural T-domains. Again, a subset of these T-domains successfully maintained indigoidine production (Fig. 9). Notably, one of our engineered IndC construcats showed an indigoidine production even higher compared to the wild-type IndC (T-domain Plu2642; Figure 3). This is particularly remarkably as our results contradict to previous studies of NRPS domains that reported the native T-domain of the indigoidine synthetase BpsA to be absolutely essential for protein function (and therefore not replaceable by other T-domains). ([Owen2012]). However, to our surprise, the BpsA T-domain-containing IndC construct did not yield any detectable indigoidine production, although BpsA shares strong sequence homology with IndC. We hypothesized, that the selection of the exact border could be critical for maintaining domain functionality when introduced into a novel NRPS module scaffold. Therefore, we amplified different BpsA T-domain variants differing in their domain border and introduced them into the IndC scaffold. Remarkably, a subset of the resulting IndC variants showed successful indigoidine production. We thus revaluated all native and synthetic T-domains in light of this finding and performed a second screening round in which we were able to rescue even more functional IndC variants, proofing our abovementioned hypothesis. We also co-transformed all engineered IndC construct bearing different natural and synthetic T-domains with four different PPTase expression constructs. To our surprise, the T-domains used not only determined general efficiency of indigoidine production, but also the efficiency of NRPS activation by the different activating PPTases. In conclusion we were able to demonstrate, that it is indeed possible to replace single Domains from NRPS modules, while preserving or even enhancing its functionality. In addition, we established an approach for the design of synthetic T-domains and proved their functionality by introducing them into the indigoidine synthetase indC scaffold. Moreover, we established a high throughput protocol for circular polymerase extension cloning and transformation (Hi-CT) (BBF RFC 99), which we applied for our domain shuffling approach. In summary, we created a library of 58 engineered indC variants. In addition we perforemd measurement of blue pigement production over time, which gave us novel insights in how NRPS domains should be designed, where the domain borders between different domains in a single NRPS module have to be set and which domains from respective NRPS pathways and bacterial strains can be used, when creating novel engineered NRPS pathways. We implemented our findings into the "NRPS-Designer" Software, so that the underlying algorithm for NRPS design takes into consideration the abovementioned findings (e.g. domain border setting) which are certainly crucial for successful in silico prediction of functional NRPSs. Thereby, our project pioneers the research on high-throughput methods for creation of synthetic NRPS modules composed of user-defined domains. We believe that our findings will highly contribute to future development of custom NRPSs.
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Figure 6: Comparison between different E. coli strains and PPTases: a) Comparison of different E. coli strains examining growth and indigoidine production The figure shows five different strains of E. coli that have been co-transformed with an indC expression plasmid and a sfp expression plasmid. The negative control is E. coli TOP10 without a plasmid. All transformants have been grown on LB agar for 48 hours at room temperature, cells were not induced. One can see that even without induction all strains express the indigoidine synthetase and produce the blue pigment indigoidine. However, the strains BAP1 and NEB Turbo grow faster in the first day, exhibiting a white phenotype (data not shown). Colonies on the plate of E. coli TOP10 are very small and dark blue/ black. Assuming that indigoidine production inhibits cell growth due to its toxicity, we concluded that TOP10 produced the most indigoidine among the strains we tested. We used E. coli TOP10 for the following experiments. b) Comparison between different PPTases concerning overall indigoidine production The Figure shows ''E. coli'' TOP10 cells co-transformed with indC and four different PPTases (sfp, svp, entD and delC), respectively. The image bottom left shows ''E. coli'' TOP10 cells without additional PPTase and the negative control is TOP10 without a plasmid.
Figure 7: Modified variants of indC with replaced T-domains We replaced the indC T-domain with both the T-domains of other native NRPS modules (entF, delH, tycC, tycA, bpsA, plu2642 and plu2670) and synthetic T-domains. The figure shows the five modified versions of indC that remain the enzyme function, thus resulting in a blue phenotype of transformed ''E. coli'' TOP10. The cells have been co-transformed with a plasmid containing the respective engineered variant of indC and a second plasmid coding for the PPTase sfp, svp, entD and delC. The figure shows representative results; the total 85 transformation results can be found in the indigoidine notebook, week 17.
Figure 8: Determination of required domain borders for T-domain exchange a) Definition of different domain border combinations for T-domain exchanges The figure shows a sequence alignment of the indC and bpsA amino acid sequences. The alignment was created using clustalO (http://www.ebi.ac.uk/Tools/msa/clustalo/) with standard parameters. The lines marked A, B and C reflect the borders we used between the A- and the T-domain, whereas those marked, 1, 2, 3 and 4 reflect the borders between the T- and the TE-domain. In total we tried all twelve combinations of a domain border {A, B, C} and a domain border {1, 2, 3, 4}, replacing the sequence inbetween with the respective part of bpsA. b) E. coli TOP10 co-transformed with modified versions of indC and the PPTase sfp The co-tranformation of the modified indC-(bpsA-T) plasmids described above with a second plasmid coding for the PPTase Sfp shows that only three domain border combinations can be used for exchanging the indC T-domain with the T-domain of bpsA. These are the combinations A1, A2 and C1. We applied combination A2 for further T-domain exchanges.
Figure 9: Applying optimized domain border combinations by T-domain exchange of native NRPS T-domains As described above, we determined an optimized domain border combination for the exchange of the native indC T-domain with the T-domain of the indigoidine synthetase bpsA. We applied this border combination (previously referred to as ''A2'') to the T-domains of the NRPS modules entF, delH4, delH5, tycA1, tycC6, plu2642 and plu2670, replacing the indC T-domain with the respective fragments of those modules. This figure shows three transformants with a blue phenotype in which the indC T-domain was exchanged by the T-domain of the respective NRPS module. The pictures were taken after 60 hours of incubation at room temperature. Once more one can see the differences in growth kinetics due to the production of indigoidine: Cells expressing the indC variant with the T-domain of bpsA grow very slow and form small and dark blue colonies, whereas cells expressing other variants grow faster. Comparing the images on the very right, we suggest that cells expressing indC with the T-domain of plu2642 produce the most indigoidine in the given timeframe, compared to both the delH4- and the bpsA-variant. The combination of the plu2642 T-domain and the indC indigoidine synthetase seems to be ideal, concerning both indigoidine production and overall growth.
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Thanks to