Team:Heidelberg/Parts

From 2013.igem.org

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Our favorite part is <a href="http://parts.igem.org/Part:BBa_K1152007">BBa_K1152007</a>: Helper construct for NRP-Indigoidine-tagging.
Our favorite part is <a href="http://parts.igem.org/Part:BBa_K1152007">BBa_K1152007</a>: Helper construct for NRP-Indigoidine-tagging.
<li> Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
<li> Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
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<li> the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
<li> the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
<li> standardization and established protocols allow for BioBrick assembly or Gibson assembly
<li> standardization and established protocols allow for BioBrick assembly or Gibson assembly
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We propose <a href="http://parts.igem.org/Part:BBa_K1152015">BBa_K1152015</a>, <a href="http://parts.igem.org/Part:BBa_K1152016">BBa_K1152016</a>, <a href="http://parts.igem.org/Part:BBa_K1152017">BBa_K1152017</a>, <a href="http://parts.igem.org/Part:BBa_K1152018">BBa_K1152018</a>, <a href="http://parts.igem.org/Part:BBa_K1152019">BBa_K1152019</a>, <a href="http://parts.igem.org/Part:BBa_K1152020">BBa_K1152020</a> as Best Part Collection, because:
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<h3 id="PartList">List of all submitted parts:</h3>
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Revision as of 02:33, 5 October 2013

Our Parts. Fascinating stuff out of the world of non-ribosomal peptides.

Team Parts

Our favorite part is BBa_K1152007: Helper construct for NRP-Indigoidine-tagging.
  • Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
  • Compatibility with custom non-ribosomal peptide (NRP) synthesis.
  • Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.

    We propose BBa_K1152013 as Best new BioBrick Part, Natural, because:
  • it encodes the original NRPS module from ''Photorhabdus luminescens'' capable of synthesizing Indigoidine
  • the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
  • standardization and established protocols allow for BioBrick assembly or Gibson assembly

    We propose BBa_K1152007 as Best new BioBrick Part or Device, Engineered, as mentioned above. We propose BBa_K1152009 as Most Improved Registry Part, because:
  • it encodes the original NRPS module from ''Photorhabdus luminescens'' capable of synthesizing Indigoidine
  • the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
  • standardization and established protocols allow for BioBrick assembly or Gibson assembly

    We propose BBa_K1152015, BBa_K1152016, BBa_K1152017, BBa_K1152018, BBa_K1152019, BBa_K1152020 as Best Part Collection, because:


  • List of all submitted parts:

    • Thanks to

    Retrieved from "http://2013.igem.org/Team:Heidelberg/Parts"