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Team:ETH Zurich/Experiments
From 2013.igem.org
(Difference between revisions)
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</table> | </table> | ||
<br clear="all"/> | <br clear="all"/> | ||
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<b>Enzyme-substrate reactions</b> | <b>Enzyme-substrate reactions</b> | ||
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<th colspan="2">Hydrolase</th> | <th colspan="2">Hydrolase</th> | ||
<th colspan="2">Complementary substrate / IUPAC name</th> | <th colspan="2">Complementary substrate / IUPAC name</th> | ||
| + | <th>Visible color</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>X-Gal</td> | <td>X-Gal</td> | ||
<td>5-Bromo-4chloro-3-indolyl-beta-galactopyranoside</td> | <td>5-Bromo-4chloro-3-indolyl-beta-galactopyranoside</td> | ||
| + | <td style="color:blue">Blue</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Green-beta-D-Gal</td> | <td>Green-beta-D-Gal</td> | ||
<td>N-Methyl-3-indolyl-beta-D_galactopyranoside</td> | <td>N-Methyl-3-indolyl-beta-D_galactopyranoside</td> | ||
| + | <td style="color:Green">Green</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Magenta glucuronide</td> | <td>Magenta glucuronide</td> | ||
<td>6-chloro-3-indolyl-beta-D-glucuronide-cycloheylammonium salt</td> | <td>6-chloro-3-indolyl-beta-D-glucuronide-cycloheylammonium salt</td> | ||
| + | <td style="color:Red">Red</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>pNPP</td> | <td>pNPP</td> | ||
<td>4-Nitrophenylphosphatedi(tris) salt</td> | <td>4-Nitrophenylphosphatedi(tris) salt</td> | ||
| + | <td style="color:Yellow">Yellow</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Magenta butyrate</td> | <td>Magenta butyrate</td> | ||
<td>5-bromo-6-chloro-3-indoxyl butyrate</td> | <td>5-bromo-6-chloro-3-indoxyl butyrate</td> | ||
| + | <td style="color:Magenta">Magenta</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>X-glucosaminide X-Glunac</td> | <td>X-glucosaminide X-Glunac</td> | ||
<td>5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide</td> | <td>5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide</td> | ||
| + | <td style="color:Lightblue">Blue</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
<br clear="all"/> | <br clear="all"/> | ||
Revision as of 13:37, 20 August 2013
Tranformation and cloning constructs
| Sender cells | Receiver cells | |
|---|---|---|
| AHL expressing system | Fluorescent reporters system | Enzyme reaction-based reporter system |
| Pcons-LuxI 1 (Strong) | Plac-LuxR-Plux-GFP | Plac-LuxR-Plux-LacZ |
| Pcons-LuxI 2 | Plac-LuxR-Plux-YFP | Plac-LuxR-Plux-Aes |
| Pcons-LuxI 3 | Plac-LuxR-Plux-CFP | Plac-LuxR-Plux-GusA |
| Pcons-LuxI 4 (Weak) | Plac-LuxR-Plux-PhoA | |
| Plac-LuxR-Plux-NagZ | ||
Enzyme-substrate reactions
The enzyme substrate reactions take less than 5 minutes and are visible by eye. Our minesweeper become better and better so keep on track for updates !
| Hydrolase | Complementary substrate / IUPAC name | Visible color | ||
|---|---|---|---|---|
| LacZ | Beta-Galactosidase | X-Gal | 5-Bromo-4chloro-3-indolyl-beta-galactopyranoside | Blue |
| LacZ | Beta-Galactosidase | Green-beta-D-Gal | N-Methyl-3-indolyl-beta-D_galactopyranoside | Green |
| GusA | Beta-glucuronidase | Magenta glucuronide | 6-chloro-3-indolyl-beta-D-glucuronide-cycloheylammonium salt | Red |
| PhoA | Alkaline phosphatase | pNPP | 4-Nitrophenylphosphatedi(tris) salt | Yellow |
| Aes | Carboxyl esterase | Magenta butyrate | 5-bromo-6-chloro-3-indoxyl butyrate | Magenta |
| NagZ | Glycoside hydrolase | X-glucosaminide X-Glunac | 5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide | Blue |
