Team:ETH Zurich/Experiments
From 2013.igem.org
Cloned constructs
Sender cells | Receiver cells | |
---|---|---|
AHL expressing system | Fluorescent reporter system | Enzyme reaction-based reporter system |
Pcons-LuxI 1 (Strong) | Plac-LuxR-Plux-GFP | Plac-LuxR-Plux-LacZ |
Pcons-LuxI 2 | Plac-LuxR-Plux-YFP | Plac-LuxR-Plux-Aes |
Pcons-LuxI 3 | Plac-LuxR-Plux-CFP | Plac-LuxR-Plux-GusA |
Pcons-LuxI 4 (Weak) | Plac-LuxR-Plux-PhoA | |
Plac-LuxR-Plux-NagZ |
Biobrick | Registry part |
---|---|
LuxI | K805016 |
LuxR | C0062 |
Pcons 1 (Strong) | J23100 |
Pcons 2 | J23118 |
Pcons 3 | J23110 |
Pcons 4 (Weak) | J23114 |
pLuxR | R0062 |
Mutagenized PLuxR_1 | Optimized Biobrick ! |
Mutagenized PLuxR_2 | Optimized Biobrick ! |
Mutagenized PLuxR_3 | Optimized Biobrick ! |
Plac | I14032 |
GFP | E0840 |
YFP | E0030 |
CFP | E0020 |
LacZ | I732006 | GusA | New Biobrick ! |
PhoA | New Biobrick ! |
Aes | New Biobrick ! |
NagZ | New Biobrick ! |