Team Parts
Our favorite part is
BBa_K1152007: Helper construct for NRP-Indigoidine-tagging.
Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
Compatibility with custom non-ribosomal peptide (NRP) synthesis.
Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
We propose BBa_K1152013 as Best new BioBrick Part, Natural, because:
it encodes the original NRPS module from ''Photorhabdus luminescens'' capable of synthesizing Indigoidine
the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
standardization and established protocols allow for BioBrick assembly or Gibson assembly
We propose BBa_K1152007 as Best new BioBrick Part or Device, Engineered, as mentioned above.
We propose BBa_K1152009 as Most Improved Registry Part, because:
it encodes the original NRPS module from ''Photorhabdus luminescens'' capable of synthesizing Indigoidine
the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
standardization and established protocols allow for BioBrick assembly or Gibson assembly
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