Natural Parts
We propose
BBa_K1152013 as Best new BioBrick Part, Natural, because:
it encodes the original NRPS module from Photorhabdus luminescens capable of synthesizing Indigoidine
the BioBrick can be combined with modules from other NRPSs to yield fusion peptides with a blue pigment tag
standardization and established protocols allow for BioBrick assembly or Gibson assembly
Engineered Parts
We propose BBa_K1152009 as Most Improved Registry Part, because:
it encodes for sfp, a 4'-Phosphopanthetheinyl-transferase (PPTase) crucial for the activation of T domains
it improved the parts BBa_K802006 and BBa_K302010, which do also encode for sfp from Bacillus subtilis but were deviating in three amino acids from the sequence published on NCBI
it is the first BioBrick in the registry that is explicitly encoding sfp, and it is annotated and characterized
We propose BBa_K1152015, BBa_K1152016, BBa_K1152017, BBa_K1152018, BBa_K1152019, BBa_K1152020 as Best Part Collection, because:
they represent a valuable ensemble of various T domains with different properties
they were designed semi-rationally based on multiple sequence alignments
they exhibit distinct synthesis rates for Indigoidine depending on the T domains of this part collection
Engineered Devices
Our favorite part is BBa_K1152007: Helper construct for NRP-Indigoidine-tagging.
Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
Compatibility with custom non-ribosomal peptide (NRP) synthesis.
Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
We propose BBa_K1152007 as Best new BioBrick Part or Device, Engineered, as mentioned above.
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