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Team:ETH Zurich/Experiments
From 2013.igem.org
Cloned constructs
| Sender cells / OHHL expressing systems |
|---|
| Pcons-LuxI (1) (Strong) |
| Pcons-LuxI (2) |
| Pcons-LuxI (3) |
| Pcons-LuxI (4) (Weak) |
| Receiver cells / OHHL detecting and processing systems | |
|---|---|
| Fluorescent reporter system | Enzyme reaction-based reporter system |
| Plac-LuxR-PluxR-GFP | Plac-LuxR-PluxR-LacZ |
| Plac-LuxR-PluxR-YFP | Plac-LuxR-PluxR-Aes |
| Plac-LuxR-PluxR-CFP | Plac-LuxR-PluxR-GusA |
| Plac-LuxR-PluxR-PhoA | |
| Plac-LuxR-PluxR-NagZ |
Final constructs
| Biobrick | Registry part |
|---|---|
| LuxI | K805016 |
| LuxR | C0062 |
| Pcons. (1) (Strong) | J23100 |
| Pcons. (2) | J23118 |
| Pcons. (3) | J23110 |
| Pcons. (4) (Weak) | J23114 |
| pLuxR | R0062 |
| Mutagenized PLuxR_1 | Optimized Biobrick ! |
| Mutagenized PLuxR_2 | Optimized Biobrick ! |
| Mutagenized PLuxR_3 | Optimized Biobrick ! |
| Plac | I14032 |
| GFP | E0840 |
| YFP | E0030 |
| CFP | E0020 |
| LacZ | I732006 |
| PhoA | J61032 |
| GusA | K330002 |
| Aes | New Biobrick ! |
| NagZ | New Biobrick ! |
| PhoA with His-Tag | Optimized Biobrick ! |
| GusA with His-Tag | Optimized Biobrick ! |
| Aes with His-Tag | Optimized Biobrick ! |
