Cloned constructs

For the final Colisweeper circuit we plan a four plasmid system. The mine cells constitutively express LuxI and NagZ. In the Non-Mine cells LuxR and PhoA are expressed constitutively whereas Aes and GusA are expressed from pLux promoters with different sensitivities. To get to this system we tested different versions of the circuit with GFP. In the following table we list all the plasmids we cloned, which biobricks we used and what experiments we used them for.

Figure 1. Transformed plasmids for mine cells and non-mine cells

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