Team:NTU-Taida/Result/Clinical Strains

Clinical Strains

After using AHL molecules as our testing targets, we start trying to use clinical species. We incubate the bacteria resistant strains overnight, centrifuge the bacteria medium, and use the supernatant(which is possible to contain AHL) as our testing sample. We use ELISA plate reader to read the fluorescent signal after 4 hours of reaction of the supernatant and the biosensors. We hope the results of clinical species provide better data for these biosensors in order to reach the market.

These strains include:

(1) Acinetobacter baumannii ATCC baa-747

(2) Pseudomonas aeruginosa PAO 1

(3) Pseudomonas aeruginosa 1009373

(4) Pseudomonas aeruginosa 2000671

(5) Pseudomonas aeruginosa 0000765-1

(6) Pseudomonas aeruginosa 0000963

(7) Pseudomonas aeruginosa 0001596

(8) Pseudomonas aeruginosa 0001819

(9) Acinetobacter baumannii 8070903

(10) Acinetobacter baumannii 0008115

(11) Escherichia coli 0000424

(12) Escherichia coli 0000703

Rhl quorum sensing

We use Rhl-mCherry(BBa_K1157006) biosensor with no positive feedback to detect quorum sensing signals of clinical species. The results are as follows. Pseudomonas has significant expression level compared to other species, and there has been different expression level between different strains of P. aeruginosa.

Las quorum sensing

We use BBa_K575024 (Las biosensor) to detect quorum sensing signals of clinical species. The results are as follows. We observed that species apart from P. aeruginosa also has response to this kind of receptor, and expression level varies among different strains.

Lux quorum sensing

We use (BBa_T9002) to detect quorum sensing signals of clinical species. The results are as follows.

PQS quorum sensing

We use PQS biosensor (the combination of BBa_ K1157017 and BBa_ K1157019) to detect quorum sensing signals of clinical species. The results are as follows. Due to the results, we think that quinolone may not only be secreted by P. aeruginosa, therefore fluorescence may also be induced by other species. According to studies, PQS may also influence the growth of other species apart from P. aeruginosa.