Since the Daci-Cluster genes of D. acidovorans, which are considered to be crucial for production of delftibactin consist of more than 58 kilo base pairs, we had to think of a method capable of trasferring this great amount of genetic information to our host organism E. coli. Bing confronted with this task, we considered the use of so called "Bacterial Artificial Chromosomes (BACs)" or "Cosmids. But finally we decided to face the challege by splitting up the crucial genes of the cluster onto convetional plasmids. One containing the gigantic gene "DelH", which comprises 18 kbp and another containing all residual parts, herein referred to as "DelRest". Additionally, we planed to integrate D. acidovorans's methyl-malonyl-CoA-pathway, which is as well necessary for succesful delftibactin production, as methyl-malonyl-CoA is a crucial co-factor for one of the PKS domains of the cluster. Since we weren't able to genomically integrate the pathway after several attempts, we decided to create a third plasmid not only containing the pathway, but as well a permeability device (BBa_...) for enabeling our bacteria to secrete of desired product into the medium. These three plasmids were constructed in different subgroups and therefore have separated labjournals. Follow the links below to read more!